Toxic effects of carbon nanotubes e titanium dioxide nanoparticles in vitro

• Autor(a) principal: Cattani, Shanda Aparecida
• Data de Publicação: 2022
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/270656
• topic: Farmácia; Nanoestruturas; Nanotubos de carbono; Estresse oxidativo; Toxicology; Nanomaterial; Carbono nanotubes; Titanium dioxide; Oxidative stress; DNA damage; Mitochondrial damage

Nanomaterials have become very attractive and useful in several areas due to their advantageous characteristics and, consequently, environmental and human exposure to these substances has increased. Nanomaterials are present throughout the environment and exposure occurs mainly via inhalation. Among the most used nanomaterials, carbon nanotubes and titanium dioxide stand out, establishing the toxicity of these substances is very important. The HUVEC cell line is multifunctional and participates in several biological processes, so this alternative in vitro model proved to be adequate. Therefore, the objective of this work was to evaluate the toxicity of carbon nanotubes, NM-401 and NM-403 and titanium dioxide, after prolonged exposure of 96 hours. The characterization of nanomaterials is essential, as the toxicity is directly influenced by the properties of the nanoparticle, for that, the analysis of particle size and polydispersion by Span and morphological and composition analysis by SEM-EDS were carried out. Cytotoxicity was evaluated by the Trypan blue assay, oxidative stress by the DCFHDA assay, genotoxicity by the alkaline comet assay and mitochondrial membrane alterations by TMRE. The results showed that the nanomaterials showed heterogeneity in particle size and homogeneity in distribution. Furthermore, it was possible to observe mitochondrial disturbances, ROS generation and DNA damage, demonstrating toxic effects after 96 h of exposure in HUVEC cells.