Intermittent hypoxia, brain glyoxalase-1 and glutathione reductase-1, and anxiety-like behavior in mice
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/200480 |
Resumo: | Objective: Sleep apnea has been associated with anxiety, but the mechanisms of the sleep apneaanxiety relationship are unresolved. Sleep apnea causes oxidative stress, which might enhance anxietylike behavior in rodents. To clarify the apnea-anxiety connection, we tested the effect of intermittent hypoxia, a model of sleep apnea, on the anxiety behavior of mice. Methods: The rodents were exposed daily to 480 one-minute cycles of intermittent hypoxia to a nadir of 761% inspiratory oxygen fraction or to a sham procedure with room air. After 7 days, the mice from both groups were placed in an elevated plus maze and were video recorded for 10 min to allow analysis of latency, frequency, and duration in open and closed arms. Glyoxalase-1 (Glo1) and glutathione reductase-1 (GR1) were measured in the cerebral cortex, hippocampus, and striatum by Western blotting. Results: Compared to controls, the intermittent hypoxia group displayed less anxiety-like behavior, perceived by a statistically significant increase in the number of entries and total time spent in open arms. A higher expression of GR1 in the cortex was also observed. Conclusion: The lack of a clear anxiety response as an outcome of intermittent hypoxia exposure suggests the existence of additional layers in the anxiety mechanism in sleep apnea, possibly represented by sleepiness and irreversible neuronal damage. |
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Carissimi, AliciaMartinez, DenisKim, Lenise JiheMartinez, Cintia Zappe FioriVieira, Luciana RodriguesRosa, Darlan Pase daPires, Gabriel Natan2019-10-10T03:50:52Z20180047-2085http://hdl.handle.net/10183/200480001102773Objective: Sleep apnea has been associated with anxiety, but the mechanisms of the sleep apneaanxiety relationship are unresolved. Sleep apnea causes oxidative stress, which might enhance anxietylike behavior in rodents. To clarify the apnea-anxiety connection, we tested the effect of intermittent hypoxia, a model of sleep apnea, on the anxiety behavior of mice. Methods: The rodents were exposed daily to 480 one-minute cycles of intermittent hypoxia to a nadir of 761% inspiratory oxygen fraction or to a sham procedure with room air. After 7 days, the mice from both groups were placed in an elevated plus maze and were video recorded for 10 min to allow analysis of latency, frequency, and duration in open and closed arms. Glyoxalase-1 (Glo1) and glutathione reductase-1 (GR1) were measured in the cerebral cortex, hippocampus, and striatum by Western blotting. Results: Compared to controls, the intermittent hypoxia group displayed less anxiety-like behavior, perceived by a statistically significant increase in the number of entries and total time spent in open arms. A higher expression of GR1 in the cortex was also observed. Conclusion: The lack of a clear anxiety response as an outcome of intermittent hypoxia exposure suggests the existence of additional layers in the anxiety mechanism in sleep apnea, possibly represented by sleepiness and irreversible neuronal damage.application/pdfengJornal brasileiro de psiquiatria. Vol. 40, no. 4 (2018), p. 376-381BiomarcadoresSíndromes da apneia do sonoHipóxia encefálicaAnsiedadeRatosLactoilglutationa liaseGlutationa redutaseEstresse oxidativoSleepBiological markersMolecular biologyStressAnxietyIntermittent hypoxia, brain glyoxalase-1 and glutathione reductase-1, and anxiety-like behavior in miceinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001102773.pdf.txt001102773.pdf.txtExtracted Texttext/plain31414http://www.lume.ufrgs.br/bitstream/10183/200480/2/001102773.pdf.txt1fd07a9156d420c41974b7c4d134c5eaMD52ORIGINAL001102773.pdfTexto completo (inglês)application/pdf316113http://www.lume.ufrgs.br/bitstream/10183/200480/1/001102773.pdf21d908931543fe7e534e374ccc4ea5e3MD5110183/2004802019-10-11 03:56:25.112434oai:www.lume.ufrgs.br:10183/200480Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-10-11T06:56:25Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Intermittent hypoxia, brain glyoxalase-1 and glutathione reductase-1, and anxiety-like behavior in mice |
title |
Intermittent hypoxia, brain glyoxalase-1 and glutathione reductase-1, and anxiety-like behavior in mice |
spellingShingle |
Intermittent hypoxia, brain glyoxalase-1 and glutathione reductase-1, and anxiety-like behavior in mice Carissimi, Alicia Biomarcadores Síndromes da apneia do sono Hipóxia encefálica Ansiedade Ratos Lactoilglutationa liase Glutationa redutase Estresse oxidativo Sleep Biological markers Molecular biology Stress Anxiety |
title_short |
Intermittent hypoxia, brain glyoxalase-1 and glutathione reductase-1, and anxiety-like behavior in mice |
title_full |
Intermittent hypoxia, brain glyoxalase-1 and glutathione reductase-1, and anxiety-like behavior in mice |
title_fullStr |
Intermittent hypoxia, brain glyoxalase-1 and glutathione reductase-1, and anxiety-like behavior in mice |
title_full_unstemmed |
Intermittent hypoxia, brain glyoxalase-1 and glutathione reductase-1, and anxiety-like behavior in mice |
title_sort |
Intermittent hypoxia, brain glyoxalase-1 and glutathione reductase-1, and anxiety-like behavior in mice |
author |
Carissimi, Alicia |
author_facet |
Carissimi, Alicia Martinez, Denis Kim, Lenise Jihe Martinez, Cintia Zappe Fiori Vieira, Luciana Rodrigues Rosa, Darlan Pase da Pires, Gabriel Natan |
author_role |
author |
author2 |
Martinez, Denis Kim, Lenise Jihe Martinez, Cintia Zappe Fiori Vieira, Luciana Rodrigues Rosa, Darlan Pase da Pires, Gabriel Natan |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Carissimi, Alicia Martinez, Denis Kim, Lenise Jihe Martinez, Cintia Zappe Fiori Vieira, Luciana Rodrigues Rosa, Darlan Pase da Pires, Gabriel Natan |
dc.subject.por.fl_str_mv |
Biomarcadores Síndromes da apneia do sono Hipóxia encefálica Ansiedade Ratos Lactoilglutationa liase Glutationa redutase Estresse oxidativo |
topic |
Biomarcadores Síndromes da apneia do sono Hipóxia encefálica Ansiedade Ratos Lactoilglutationa liase Glutationa redutase Estresse oxidativo Sleep Biological markers Molecular biology Stress Anxiety |
dc.subject.eng.fl_str_mv |
Sleep Biological markers Molecular biology Stress Anxiety |
description |
Objective: Sleep apnea has been associated with anxiety, but the mechanisms of the sleep apneaanxiety relationship are unresolved. Sleep apnea causes oxidative stress, which might enhance anxietylike behavior in rodents. To clarify the apnea-anxiety connection, we tested the effect of intermittent hypoxia, a model of sleep apnea, on the anxiety behavior of mice. Methods: The rodents were exposed daily to 480 one-minute cycles of intermittent hypoxia to a nadir of 761% inspiratory oxygen fraction or to a sham procedure with room air. After 7 days, the mice from both groups were placed in an elevated plus maze and were video recorded for 10 min to allow analysis of latency, frequency, and duration in open and closed arms. Glyoxalase-1 (Glo1) and glutathione reductase-1 (GR1) were measured in the cerebral cortex, hippocampus, and striatum by Western blotting. Results: Compared to controls, the intermittent hypoxia group displayed less anxiety-like behavior, perceived by a statistically significant increase in the number of entries and total time spent in open arms. A higher expression of GR1 in the cortex was also observed. Conclusion: The lack of a clear anxiety response as an outcome of intermittent hypoxia exposure suggests the existence of additional layers in the anxiety mechanism in sleep apnea, possibly represented by sleepiness and irreversible neuronal damage. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
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2019-10-10T03:50:52Z |
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http://hdl.handle.net/10183/200480 |
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0047-2085 |
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001102773 |
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http://hdl.handle.net/10183/200480 |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Jornal brasileiro de psiquiatria. Vol. 40, no. 4 (2018), p. 376-381 |
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