Transplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in wistar rats
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/87765 |
Resumo: | Cell transplantation is a promising experimental treatment for spinal cord injury. The aim of the present study was to evaluate the efficacy of mononuclear cells from human umbilical cord blood in promoting functional recovery when transplanted after a contusion spinal cord injury. Female Wistar rats (12 weeks old) were submitted to spinal injury with a MASCIS impactor and divided into 4 groups: control, surgical control, spinal cord injury, and one cell-treated lesion group. Mononuclear cells from umbilical cord blood of human male neonates were transplanted in two experiments: a) 1 h after surgery, into the injury site at a concentration of 5 x 106 cells diluted in 10 μL 0.9% NaCl (N = 8-10 per group); b) into the cisterna magna, 9 days after lesion at a concentration of 5 x 106 cells diluted in 150 μL 0.9% NaCl (N = 12-14 per group). The transplanted animals were immunosuppressed with cyclosporin-A (10 mg/kg per day). The BBB scale was used to evaluate motor behavior and the injury site was analyzed with immunofluorescent markers to label human transplanted cells, oligodendrocytes, neurons, and astrocytes. Spinal cord injury rats had 25% loss of cord tissue and cell treatment did not affect lesion extension. Transplanted cells survived in the injured area for 6 weeks after the procedure and both transplanted groups showed better motor recovery than the untreated ones (P < 0.05). The transplantation of mononuclear cells from human umbilical cord blood promoted functional recovery with no evidence of cell differentiation. |
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Rodrigues, Luciano PalmeiroBraghirolli, Daikelly IglesiasNicola, Fabrício do CoutoZanatta, GeancarloSteffens, DanielaValentim, Lauren MartinsWitczak, AlessandroNetto, Carlos AlexandrePranke, Patricia Helena LucasAchaval-Elena, Matilde2014-02-26T01:51:44Z20120100-879Xhttp://hdl.handle.net/10183/87765000822292Cell transplantation is a promising experimental treatment for spinal cord injury. The aim of the present study was to evaluate the efficacy of mononuclear cells from human umbilical cord blood in promoting functional recovery when transplanted after a contusion spinal cord injury. Female Wistar rats (12 weeks old) were submitted to spinal injury with a MASCIS impactor and divided into 4 groups: control, surgical control, spinal cord injury, and one cell-treated lesion group. Mononuclear cells from umbilical cord blood of human male neonates were transplanted in two experiments: a) 1 h after surgery, into the injury site at a concentration of 5 x 106 cells diluted in 10 μL 0.9% NaCl (N = 8-10 per group); b) into the cisterna magna, 9 days after lesion at a concentration of 5 x 106 cells diluted in 150 μL 0.9% NaCl (N = 12-14 per group). The transplanted animals were immunosuppressed with cyclosporin-A (10 mg/kg per day). The BBB scale was used to evaluate motor behavior and the injury site was analyzed with immunofluorescent markers to label human transplanted cells, oligodendrocytes, neurons, and astrocytes. Spinal cord injury rats had 25% loss of cord tissue and cell treatment did not affect lesion extension. Transplanted cells survived in the injured area for 6 weeks after the procedure and both transplanted groups showed better motor recovery than the untreated ones (P < 0.05). The transplantation of mononuclear cells from human umbilical cord blood promoted functional recovery with no evidence of cell differentiation.application/pdfengBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. São Paulo. Vol. 45, n. 1 (Jan. 2012), p. 49-57Medula espinalTransplante de células-tronco de sangue do cordão umbilicalSpinal cord injuryCell therapyFunctional recoveryMononuclear cellsHuman umbilical cord bloodTransplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in wistar ratsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000822292.pdf000822292.pdfTexto completo (inglês)application/pdf3825926http://www.lume.ufrgs.br/bitstream/10183/87765/1/000822292.pdf97add625115e6bc180071b6110c28485MD51TEXT000822292.pdf.txt000822292.pdf.txtExtracted Texttext/plain34617http://www.lume.ufrgs.br/bitstream/10183/87765/2/000822292.pdf.txte9f309ba8596764009417c0d27efcc44MD52THUMBNAIL000822292.pdf.jpg000822292.pdf.jpgGenerated Thumbnailimage/jpeg2204http://www.lume.ufrgs.br/bitstream/10183/87765/3/000822292.pdf.jpge8ee7613faa3078d36e24e1522f654e3MD5310183/877652021-07-09 04:40:06.686789oai:www.lume.ufrgs.br:10183/87765Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-07-09T07:40:06Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Transplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in wistar rats |
title |
Transplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in wistar rats |
spellingShingle |
Transplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in wistar rats Rodrigues, Luciano Palmeiro Medula espinal Transplante de células-tronco de sangue do cordão umbilical Spinal cord injury Cell therapy Functional recovery Mononuclear cells Human umbilical cord blood |
title_short |
Transplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in wistar rats |
title_full |
Transplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in wistar rats |
title_fullStr |
Transplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in wistar rats |
title_full_unstemmed |
Transplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in wistar rats |
title_sort |
Transplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in wistar rats |
author |
Rodrigues, Luciano Palmeiro |
author_facet |
Rodrigues, Luciano Palmeiro Braghirolli, Daikelly Iglesias Nicola, Fabrício do Couto Zanatta, Geancarlo Steffens, Daniela Valentim, Lauren Martins Witczak, Alessandro Netto, Carlos Alexandre Pranke, Patricia Helena Lucas Achaval-Elena, Matilde |
author_role |
author |
author2 |
Braghirolli, Daikelly Iglesias Nicola, Fabrício do Couto Zanatta, Geancarlo Steffens, Daniela Valentim, Lauren Martins Witczak, Alessandro Netto, Carlos Alexandre Pranke, Patricia Helena Lucas Achaval-Elena, Matilde |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Rodrigues, Luciano Palmeiro Braghirolli, Daikelly Iglesias Nicola, Fabrício do Couto Zanatta, Geancarlo Steffens, Daniela Valentim, Lauren Martins Witczak, Alessandro Netto, Carlos Alexandre Pranke, Patricia Helena Lucas Achaval-Elena, Matilde |
dc.subject.por.fl_str_mv |
Medula espinal Transplante de células-tronco de sangue do cordão umbilical |
topic |
Medula espinal Transplante de células-tronco de sangue do cordão umbilical Spinal cord injury Cell therapy Functional recovery Mononuclear cells Human umbilical cord blood |
dc.subject.eng.fl_str_mv |
Spinal cord injury Cell therapy Functional recovery Mononuclear cells Human umbilical cord blood |
description |
Cell transplantation is a promising experimental treatment for spinal cord injury. The aim of the present study was to evaluate the efficacy of mononuclear cells from human umbilical cord blood in promoting functional recovery when transplanted after a contusion spinal cord injury. Female Wistar rats (12 weeks old) were submitted to spinal injury with a MASCIS impactor and divided into 4 groups: control, surgical control, spinal cord injury, and one cell-treated lesion group. Mononuclear cells from umbilical cord blood of human male neonates were transplanted in two experiments: a) 1 h after surgery, into the injury site at a concentration of 5 x 106 cells diluted in 10 μL 0.9% NaCl (N = 8-10 per group); b) into the cisterna magna, 9 days after lesion at a concentration of 5 x 106 cells diluted in 150 μL 0.9% NaCl (N = 12-14 per group). The transplanted animals were immunosuppressed with cyclosporin-A (10 mg/kg per day). The BBB scale was used to evaluate motor behavior and the injury site was analyzed with immunofluorescent markers to label human transplanted cells, oligodendrocytes, neurons, and astrocytes. Spinal cord injury rats had 25% loss of cord tissue and cell treatment did not affect lesion extension. Transplanted cells survived in the injured area for 6 weeks after the procedure and both transplanted groups showed better motor recovery than the untreated ones (P < 0.05). The transplantation of mononuclear cells from human umbilical cord blood promoted functional recovery with no evidence of cell differentiation. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.accessioned.fl_str_mv |
2014-02-26T01:51:44Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/87765 |
dc.identifier.issn.pt_BR.fl_str_mv |
0100-879X |
dc.identifier.nrb.pt_BR.fl_str_mv |
000822292 |
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http://hdl.handle.net/10183/87765 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. São Paulo. Vol. 45, n. 1 (Jan. 2012), p. 49-57 |
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