Determination of the mode of reproduction of bahiagrass hybrids using cytoembryological analysis and molecular markers
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Publication Date: | 2017 |
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Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UFRGS |
Download full: | http://hdl.handle.net/10183/185934 |
Summary: | The aim of this study was to determine the mode of reproduction of a hybrid progeny derived from intraspecific crosses of Paspalum notatum through cytoembryological analysis and use of RAPD (random amplification of polymorphic DNA) molecular markers. Cytoembryological analysis allowed identification of the mode of reproduction of 28 plants that were selected after agronomic productivity evaluations. Of these, 19 had embryo sac morphology compatible with an apomictic mode of reproduction and nine had embryo sac morphology compatible with a sexual mode of reproduction. Meanwhile, molecular marker analysis for 194 individuals showed 54 sexual and 140 apomictic plants; of the 28 plants analyzed by the two methods, ten results (35.7%) were in disagreement. In this paper, through cytoembryological analyses, a ratio of 1:2.1 of sexual to apomictic plants was found. The BCU 243 marker showed a stable pattern of amplification, but some results differed with cytoembryological analyses, demonstrating that these analyses are more reliable when determining the mode of sexual reproduction for the plants of P. notatum. Apomictic plants characterized in this work can be tested in the field to check their agronomic value and registration as plant varieties, while the sexual plants can be used as potential parents in future crosses. |
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Weiler, Roberto LuisDall'Agnol, MiguelSimioni, CarineKrycki, Karine CristinaDahmer, NairGuerra, Divanilde2018-12-05T02:44:16Z20171516-3598http://hdl.handle.net/10183/185934001079288The aim of this study was to determine the mode of reproduction of a hybrid progeny derived from intraspecific crosses of Paspalum notatum through cytoembryological analysis and use of RAPD (random amplification of polymorphic DNA) molecular markers. Cytoembryological analysis allowed identification of the mode of reproduction of 28 plants that were selected after agronomic productivity evaluations. Of these, 19 had embryo sac morphology compatible with an apomictic mode of reproduction and nine had embryo sac morphology compatible with a sexual mode of reproduction. Meanwhile, molecular marker analysis for 194 individuals showed 54 sexual and 140 apomictic plants; of the 28 plants analyzed by the two methods, ten results (35.7%) were in disagreement. In this paper, through cytoembryological analyses, a ratio of 1:2.1 of sexual to apomictic plants was found. The BCU 243 marker showed a stable pattern of amplification, but some results differed with cytoembryological analyses, demonstrating that these analyses are more reliable when determining the mode of sexual reproduction for the plants of P. notatum. Apomictic plants characterized in this work can be tested in the field to check their agronomic value and registration as plant varieties, while the sexual plants can be used as potential parents in future crosses.application/pdfengRevista brasileira de zootecnia. Viçosa, MG. Vol. 46, n.3 (mar. 2017), p. 185-191Melhoramento genético vegetalMarcador molecularReprodução vegetalCapim brachiariaPaspalum notatumApomixiaApomixisMolecular analysisPaspalum notatumPlant breedingRAPDDetermination of the mode of reproduction of bahiagrass hybrids using cytoembryological analysis and molecular markersinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001079288.pdf.txt001079288.pdf.txtExtracted Texttext/plain29244http://www.lume.ufrgs.br/bitstream/10183/185934/2/001079288.pdf.txt825960cf8ae89cc6194dbb1e848550dbMD52ORIGINAL001079288.pdfTexto completo (inglês)application/pdf539499http://www.lume.ufrgs.br/bitstream/10183/185934/1/001079288.pdf72dcd9fd1001e8cc967d55d566f59395MD5110183/1859342019-02-15 02:34:04.93566oai:www.lume.ufrgs.br:10183/185934Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-02-15T04:34:04Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Determination of the mode of reproduction of bahiagrass hybrids using cytoembryological analysis and molecular markers |
title |
Determination of the mode of reproduction of bahiagrass hybrids using cytoembryological analysis and molecular markers |
spellingShingle |
Determination of the mode of reproduction of bahiagrass hybrids using cytoembryological analysis and molecular markers Weiler, Roberto Luis Melhoramento genético vegetal Marcador molecular Reprodução vegetal Capim brachiaria Paspalum notatum Apomixia Apomixis Molecular analysis Paspalum notatum Plant breeding RAPD |
title_short |
Determination of the mode of reproduction of bahiagrass hybrids using cytoembryological analysis and molecular markers |
title_full |
Determination of the mode of reproduction of bahiagrass hybrids using cytoembryological analysis and molecular markers |
title_fullStr |
Determination of the mode of reproduction of bahiagrass hybrids using cytoembryological analysis and molecular markers |
title_full_unstemmed |
Determination of the mode of reproduction of bahiagrass hybrids using cytoembryological analysis and molecular markers |
title_sort |
Determination of the mode of reproduction of bahiagrass hybrids using cytoembryological analysis and molecular markers |
author |
Weiler, Roberto Luis |
author_facet |
Weiler, Roberto Luis Dall'Agnol, Miguel Simioni, Carine Krycki, Karine Cristina Dahmer, Nair Guerra, Divanilde |
author_role |
author |
author2 |
Dall'Agnol, Miguel Simioni, Carine Krycki, Karine Cristina Dahmer, Nair Guerra, Divanilde |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Weiler, Roberto Luis Dall'Agnol, Miguel Simioni, Carine Krycki, Karine Cristina Dahmer, Nair Guerra, Divanilde |
dc.subject.por.fl_str_mv |
Melhoramento genético vegetal Marcador molecular Reprodução vegetal Capim brachiaria Paspalum notatum Apomixia |
topic |
Melhoramento genético vegetal Marcador molecular Reprodução vegetal Capim brachiaria Paspalum notatum Apomixia Apomixis Molecular analysis Paspalum notatum Plant breeding RAPD |
dc.subject.eng.fl_str_mv |
Apomixis Molecular analysis Paspalum notatum Plant breeding RAPD |
description |
The aim of this study was to determine the mode of reproduction of a hybrid progeny derived from intraspecific crosses of Paspalum notatum through cytoembryological analysis and use of RAPD (random amplification of polymorphic DNA) molecular markers. Cytoembryological analysis allowed identification of the mode of reproduction of 28 plants that were selected after agronomic productivity evaluations. Of these, 19 had embryo sac morphology compatible with an apomictic mode of reproduction and nine had embryo sac morphology compatible with a sexual mode of reproduction. Meanwhile, molecular marker analysis for 194 individuals showed 54 sexual and 140 apomictic plants; of the 28 plants analyzed by the two methods, ten results (35.7%) were in disagreement. In this paper, through cytoembryological analyses, a ratio of 1:2.1 of sexual to apomictic plants was found. The BCU 243 marker showed a stable pattern of amplification, but some results differed with cytoembryological analyses, demonstrating that these analyses are more reliable when determining the mode of sexual reproduction for the plants of P. notatum. Apomictic plants characterized in this work can be tested in the field to check their agronomic value and registration as plant varieties, while the sexual plants can be used as potential parents in future crosses. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2018-12-05T02:44:16Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
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info:eu-repo/semantics/publishedVersion |
format |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/185934 |
dc.identifier.issn.pt_BR.fl_str_mv |
1516-3598 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001079288 |
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url |
http://hdl.handle.net/10183/185934 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Revista brasileira de zootecnia. Viçosa, MG. Vol. 46, n.3 (mar. 2017), p. 185-191 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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