Insulin receptor tyrosine kinase activity in colon carcinoma
Autor(a) principal: | |
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Data de Publicação: | 1996 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/247453 |
Resumo: | Colon carcinoma is the most common tumor of the gastrointestinal tract. According to some investigators, insulin, epidermal growth factor (EGF) and insulin-like growth factor I (IGF-I) may be involved in the neoplastic proliferation. Insulin-binding and receptor tyrosine kinase activity were investigated in colon carcinomas and in normal colons. The insulin receptor concentration, as shown by binding assays, was 17.4 ± 4.3 fmol/J.Lg in normal colon and 29.69 ± 9.4 fmol/ J.Lg in colon carcinoma. Nevertheless, the insulin affinity of the receptor was similar in both groups (Kd ::: 1 nM). Both normal and neoplastic colon showed phosphorylation of the insulin receptor. The electrophoretic migration of the B-subunit of the insulin receptors purified from colon carcinomas was similar to that of normal colon and both tissues demonstrated an insulin-dependent autophosphorylation. The receptor tyrosine kinase activity was measured by the incorporation of[gamma32P]ATP into the B-subunit. The basal and the insulin-stimulated tyrosine kinase activities were significantly higher in colon carcinomas compared to normal colon tissues (2.2 and 1.6 times, respectively). Understanding the metabolism of neoplastic cells may contribute to the development of prevention strategies as well as new therapies. It is now necessary to study other steps of the insulin signal transduction pathway, such as insulin receptor substrate 1 phosphorylation. |
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Corleta, Helena von EyeCapp, EdisonCorleta, Oly Campos2022-08-19T04:45:36Z19960100-879Xhttp://hdl.handle.net/10183/247453000302141Colon carcinoma is the most common tumor of the gastrointestinal tract. According to some investigators, insulin, epidermal growth factor (EGF) and insulin-like growth factor I (IGF-I) may be involved in the neoplastic proliferation. Insulin-binding and receptor tyrosine kinase activity were investigated in colon carcinomas and in normal colons. The insulin receptor concentration, as shown by binding assays, was 17.4 ± 4.3 fmol/J.Lg in normal colon and 29.69 ± 9.4 fmol/ J.Lg in colon carcinoma. Nevertheless, the insulin affinity of the receptor was similar in both groups (Kd ::: 1 nM). Both normal and neoplastic colon showed phosphorylation of the insulin receptor. The electrophoretic migration of the B-subunit of the insulin receptors purified from colon carcinomas was similar to that of normal colon and both tissues demonstrated an insulin-dependent autophosphorylation. The receptor tyrosine kinase activity was measured by the incorporation of[gamma32P]ATP into the B-subunit. The basal and the insulin-stimulated tyrosine kinase activities were significantly higher in colon carcinomas compared to normal colon tissues (2.2 and 1.6 times, respectively). Understanding the metabolism of neoplastic cells may contribute to the development of prevention strategies as well as new therapies. It is now necessary to study other steps of the insulin signal transduction pathway, such as insulin receptor substrate 1 phosphorylation.application/pdfengBrazilian Journal of Medical and Biological Research. Vol. 29, no. 12 (1996), p. 1593-1597Neoplasias do coloInsulinaFator de crescimento insulin-like-IReceptores proteína tirosina quinasesColon carcinomaInsulin receptorTyrosine kinaseInsulin signalingInsulin receptor tyrosine kinase activity in colon carcinomainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000302141.pdf.txt000302141.pdf.txtExtracted Texttext/plain16967http://www.lume.ufrgs.br/bitstream/10183/247453/2/000302141.pdf.txtab974495a482cd7dc44b914b0ab5de47MD52ORIGINAL000302141.pdfTexto completo (inglês)application/pdf2780747http://www.lume.ufrgs.br/bitstream/10183/247453/1/000302141.pdf2a5d0344c4a1dd2c6a3cd9decf4b53f1MD5110183/2474532023-08-18 03:39:33.770174oai:www.lume.ufrgs.br:10183/247453Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-08-18T06:39:33Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Insulin receptor tyrosine kinase activity in colon carcinoma |
title |
Insulin receptor tyrosine kinase activity in colon carcinoma |
spellingShingle |
Insulin receptor tyrosine kinase activity in colon carcinoma Corleta, Helena von Eye Neoplasias do colo Insulina Fator de crescimento insulin-like-I Receptores proteína tirosina quinases Colon carcinoma Insulin receptor Tyrosine kinase Insulin signaling |
title_short |
Insulin receptor tyrosine kinase activity in colon carcinoma |
title_full |
Insulin receptor tyrosine kinase activity in colon carcinoma |
title_fullStr |
Insulin receptor tyrosine kinase activity in colon carcinoma |
title_full_unstemmed |
Insulin receptor tyrosine kinase activity in colon carcinoma |
title_sort |
Insulin receptor tyrosine kinase activity in colon carcinoma |
author |
Corleta, Helena von Eye |
author_facet |
Corleta, Helena von Eye Capp, Edison Corleta, Oly Campos |
author_role |
author |
author2 |
Capp, Edison Corleta, Oly Campos |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Corleta, Helena von Eye Capp, Edison Corleta, Oly Campos |
dc.subject.por.fl_str_mv |
Neoplasias do colo Insulina Fator de crescimento insulin-like-I Receptores proteína tirosina quinases |
topic |
Neoplasias do colo Insulina Fator de crescimento insulin-like-I Receptores proteína tirosina quinases Colon carcinoma Insulin receptor Tyrosine kinase Insulin signaling |
dc.subject.eng.fl_str_mv |
Colon carcinoma Insulin receptor Tyrosine kinase Insulin signaling |
description |
Colon carcinoma is the most common tumor of the gastrointestinal tract. According to some investigators, insulin, epidermal growth factor (EGF) and insulin-like growth factor I (IGF-I) may be involved in the neoplastic proliferation. Insulin-binding and receptor tyrosine kinase activity were investigated in colon carcinomas and in normal colons. The insulin receptor concentration, as shown by binding assays, was 17.4 ± 4.3 fmol/J.Lg in normal colon and 29.69 ± 9.4 fmol/ J.Lg in colon carcinoma. Nevertheless, the insulin affinity of the receptor was similar in both groups (Kd ::: 1 nM). Both normal and neoplastic colon showed phosphorylation of the insulin receptor. The electrophoretic migration of the B-subunit of the insulin receptors purified from colon carcinomas was similar to that of normal colon and both tissues demonstrated an insulin-dependent autophosphorylation. The receptor tyrosine kinase activity was measured by the incorporation of[gamma32P]ATP into the B-subunit. The basal and the insulin-stimulated tyrosine kinase activities were significantly higher in colon carcinomas compared to normal colon tissues (2.2 and 1.6 times, respectively). Understanding the metabolism of neoplastic cells may contribute to the development of prevention strategies as well as new therapies. It is now necessary to study other steps of the insulin signal transduction pathway, such as insulin receptor substrate 1 phosphorylation. |
publishDate |
1996 |
dc.date.issued.fl_str_mv |
1996 |
dc.date.accessioned.fl_str_mv |
2022-08-19T04:45:36Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
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http://hdl.handle.net/10183/247453 |
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0100-879X |
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000302141 |
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http://hdl.handle.net/10183/247453 |
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eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian Journal of Medical and Biological Research. Vol. 29, no. 12 (1996), p. 1593-1597 |
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openAccess |
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