Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/152693 |
Resumo: | Spinal cord injury (SCI) is a disabling condition resulting in deficits of sensory and motor functions, and has no effective treatment. Considering that protocols with stem cell transplantation and treadmill training have shown promising results, the present study evaluated the effectiveness of stem cells from human exfoliated deciduous teeth (SHEDs) transplantation combined with treadmill training in rats with experimental spinal cord injury. Fifty-four Wistar rats were spinalized using NYU impactor. The rats were randomly distributed into 5 groups: Sham (laminectomy with no SCI, n=10); SCI (laminectomy followed by SCI, n=12); SHEDs (SCI treated with SHEDs, n=11); TT (SCI treated with treadmill training, n=11); SHEDs+TT (SCI treated with SHEDs and treadmill training; n=10). Treatment with SHEDs alone or in combination with treadmill training promoted functional recovery, reaching scores of 15 and 14, respectively, in the BBB scale, being different from the SCI group, which reached 11. SHEDs treatment was able to reduce the cystic cavity area and glial scar, increase neurofilament. Treadmill training alone had no functional effectiveness or tissue effects. In a second experiment, the SHEDs transplantation reduced the TNF-a levels in the cord tissue measured 6 h after the injury. Contrary to our hypothesis, treadmill training either alone or in combination, caused no functional improvement. However, SHEDs showed to be neuroprotective, by the reduction of TNF-a levels, the cystic cavity and the glial scar associated with the improvement of motor function after SCI. These results provide evidence that grafted SHEDs might be an effective therapy to spinal cord lesions, with possible anti-inflammatory action. |
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Nicola, Fabrício do CoutoRodrigues, Luciano PalmeiroCrestani, ThayaneQuintiliano, KerlinSanches, Eduardo FariasPereira, Suelen WillbornAristimunha, Dirceu CardosoBoisserand, Lígia Simões BragaPranke, Patricia Helena LucasNetto, Carlos Alexandre2017-02-17T02:33:06Z20160100-879Xhttp://hdl.handle.net/10183/152693001009147Spinal cord injury (SCI) is a disabling condition resulting in deficits of sensory and motor functions, and has no effective treatment. Considering that protocols with stem cell transplantation and treadmill training have shown promising results, the present study evaluated the effectiveness of stem cells from human exfoliated deciduous teeth (SHEDs) transplantation combined with treadmill training in rats with experimental spinal cord injury. Fifty-four Wistar rats were spinalized using NYU impactor. The rats were randomly distributed into 5 groups: Sham (laminectomy with no SCI, n=10); SCI (laminectomy followed by SCI, n=12); SHEDs (SCI treated with SHEDs, n=11); TT (SCI treated with treadmill training, n=11); SHEDs+TT (SCI treated with SHEDs and treadmill training; n=10). Treatment with SHEDs alone or in combination with treadmill training promoted functional recovery, reaching scores of 15 and 14, respectively, in the BBB scale, being different from the SCI group, which reached 11. SHEDs treatment was able to reduce the cystic cavity area and glial scar, increase neurofilament. Treadmill training alone had no functional effectiveness or tissue effects. In a second experiment, the SHEDs transplantation reduced the TNF-a levels in the cord tissue measured 6 h after the injury. Contrary to our hypothesis, treadmill training either alone or in combination, caused no functional improvement. However, SHEDs showed to be neuroprotective, by the reduction of TNF-a levels, the cystic cavity and the glial scar associated with the improvement of motor function after SCI. These results provide evidence that grafted SHEDs might be an effective therapy to spinal cord lesions, with possible anti-inflammatory action.application/pdfengBrazilian journal of medical and biological research. Ribeirão Preto. Vol. 49, n. 9 (2016), 11 p.Traumatismos da medula espinalCélulas-troncoTerapia por exercícioSpinal cord injuryStem cellsTreadmill trainingFunctional recoveryMASCIS impactorHuman dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injuryEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001009147.pdf001009147.pdfTexto completo (inglês)application/pdf2349526http://www.lume.ufrgs.br/bitstream/10183/152693/1/001009147.pdfcaa7ce9639c45f0a5e749a694bbca381MD51TEXT001009147.pdf.txt001009147.pdf.txtExtracted Texttext/plain41782http://www.lume.ufrgs.br/bitstream/10183/152693/2/001009147.pdf.txtcdb8b75f70c08aeb4cd9d52bdd633a9bMD5210183/1526932017-10-20 02:26:44.615158oai:www.lume.ufrgs.br:10183/152693Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2017-10-20T04:26:44Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury |
title |
Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury |
spellingShingle |
Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury Nicola, Fabrício do Couto Traumatismos da medula espinal Células-tronco Terapia por exercício Spinal cord injury Stem cells Treadmill training Functional recovery MASCIS impactor |
title_short |
Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury |
title_full |
Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury |
title_fullStr |
Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury |
title_full_unstemmed |
Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury |
title_sort |
Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury |
author |
Nicola, Fabrício do Couto |
author_facet |
Nicola, Fabrício do Couto Rodrigues, Luciano Palmeiro Crestani, Thayane Quintiliano, Kerlin Sanches, Eduardo Farias Pereira, Suelen Willborn Aristimunha, Dirceu Cardoso Boisserand, Lígia Simões Braga Pranke, Patricia Helena Lucas Netto, Carlos Alexandre |
author_role |
author |
author2 |
Rodrigues, Luciano Palmeiro Crestani, Thayane Quintiliano, Kerlin Sanches, Eduardo Farias Pereira, Suelen Willborn Aristimunha, Dirceu Cardoso Boisserand, Lígia Simões Braga Pranke, Patricia Helena Lucas Netto, Carlos Alexandre |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Nicola, Fabrício do Couto Rodrigues, Luciano Palmeiro Crestani, Thayane Quintiliano, Kerlin Sanches, Eduardo Farias Pereira, Suelen Willborn Aristimunha, Dirceu Cardoso Boisserand, Lígia Simões Braga Pranke, Patricia Helena Lucas Netto, Carlos Alexandre |
dc.subject.por.fl_str_mv |
Traumatismos da medula espinal Células-tronco Terapia por exercício |
topic |
Traumatismos da medula espinal Células-tronco Terapia por exercício Spinal cord injury Stem cells Treadmill training Functional recovery MASCIS impactor |
dc.subject.eng.fl_str_mv |
Spinal cord injury Stem cells Treadmill training Functional recovery MASCIS impactor |
description |
Spinal cord injury (SCI) is a disabling condition resulting in deficits of sensory and motor functions, and has no effective treatment. Considering that protocols with stem cell transplantation and treadmill training have shown promising results, the present study evaluated the effectiveness of stem cells from human exfoliated deciduous teeth (SHEDs) transplantation combined with treadmill training in rats with experimental spinal cord injury. Fifty-four Wistar rats were spinalized using NYU impactor. The rats were randomly distributed into 5 groups: Sham (laminectomy with no SCI, n=10); SCI (laminectomy followed by SCI, n=12); SHEDs (SCI treated with SHEDs, n=11); TT (SCI treated with treadmill training, n=11); SHEDs+TT (SCI treated with SHEDs and treadmill training; n=10). Treatment with SHEDs alone or in combination with treadmill training promoted functional recovery, reaching scores of 15 and 14, respectively, in the BBB scale, being different from the SCI group, which reached 11. SHEDs treatment was able to reduce the cystic cavity area and glial scar, increase neurofilament. Treadmill training alone had no functional effectiveness or tissue effects. In a second experiment, the SHEDs transplantation reduced the TNF-a levels in the cord tissue measured 6 h after the injury. Contrary to our hypothesis, treadmill training either alone or in combination, caused no functional improvement. However, SHEDs showed to be neuroprotective, by the reduction of TNF-a levels, the cystic cavity and the glial scar associated with the improvement of motor function after SCI. These results provide evidence that grafted SHEDs might be an effective therapy to spinal cord lesions, with possible anti-inflammatory action. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2017-02-17T02:33:06Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/152693 |
dc.identifier.issn.pt_BR.fl_str_mv |
0100-879X |
dc.identifier.nrb.pt_BR.fl_str_mv |
001009147 |
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0100-879X 001009147 |
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http://hdl.handle.net/10183/152693 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of medical and biological research. Ribeirão Preto. Vol. 49, n. 9 (2016), 11 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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