Characterization of ectonucleotidases in human medulloblastoma cell lines : ecto-5´NT/CD73 in metastasis as potential prognostic factor
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/225259 |
Resumo: | Medulloblastoma (MB) is the most common malignant brain tumor in children and occurs mainly in the cerebellum. Important intracellular signaling molecules, such those present in the Sonic Hedgehog and Wnt pathways, are involved in its development and can also be employed to determine tumor grade and prognosis. Ectonucleotidases, particularly ecto59NT/CD73, are important enzymes in the malignant process of different tumor types regulating extracellular ATP and adenosine levels. Here, we investigated the activity of ectonucleotidases in three malignant human cell lines: Daoy and ONS76, being representative of primary MB, and the D283 cell line, derived from a metastatic MB. All cell lines secreted ATP into the extracellular medium while hydrolyze poorly this nucleotide, which is in agreement with the low expression and activity of pyrophosphate/phosphodiesterase, NTPDases and alkaline phosphatase. The analysis of AMP hydrolysis showed that Daoy and ONS76 completely hydrolyzed AMP, with parallel adenosine production (Daoy) and inosine accumulation (ONS76). On the other hand, D283 cell line did not hydrolyze AMP. Moreover, primary MB tumor cells, Daoy and ONS76 express the ecto-59NT/CD73 while D283 representative of a metastatic tumor, revealed poor expression of this enzyme, while the ecto-adenosine deaminase showed higher expression in D283 compared to Daoy and ONS76 cells. Nuclear betacatenin has been suggested as a marker for MB prognosis. Further it can promotes expression of ecto-59NT/CD73 and suppression of adenosine deaminase. It was observed that Daoy and ONS76 showed greater nuclear beta-catenin immunoreactivity than D283, which presented mainly cytoplasmic immunoreactivity. In summary, the absence of ecto59NT/CD73 in the D283 cell line, a metastatic MB phenotype, suggests that high expression levels of this ectonucleotidase could be correlated with a poor prognosis in patients with MB. |
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Cappellari, Angélica ReginaRockenbach, LilianaDietrich, FabríciaClarimundo, Vanessa SchuckGlaser, TalitaBraganhol, ElizandraAbujamra, Ana LúciaRoesler, RafaelUlrich, HenningBattastini, Ana Maria Oliveira2021-08-06T04:41:12Z20121932-6203http://hdl.handle.net/10183/225259000876706Medulloblastoma (MB) is the most common malignant brain tumor in children and occurs mainly in the cerebellum. Important intracellular signaling molecules, such those present in the Sonic Hedgehog and Wnt pathways, are involved in its development and can also be employed to determine tumor grade and prognosis. Ectonucleotidases, particularly ecto59NT/CD73, are important enzymes in the malignant process of different tumor types regulating extracellular ATP and adenosine levels. Here, we investigated the activity of ectonucleotidases in three malignant human cell lines: Daoy and ONS76, being representative of primary MB, and the D283 cell line, derived from a metastatic MB. All cell lines secreted ATP into the extracellular medium while hydrolyze poorly this nucleotide, which is in agreement with the low expression and activity of pyrophosphate/phosphodiesterase, NTPDases and alkaline phosphatase. The analysis of AMP hydrolysis showed that Daoy and ONS76 completely hydrolyzed AMP, with parallel adenosine production (Daoy) and inosine accumulation (ONS76). On the other hand, D283 cell line did not hydrolyze AMP. Moreover, primary MB tumor cells, Daoy and ONS76 express the ecto-59NT/CD73 while D283 representative of a metastatic tumor, revealed poor expression of this enzyme, while the ecto-adenosine deaminase showed higher expression in D283 compared to Daoy and ONS76 cells. Nuclear betacatenin has been suggested as a marker for MB prognosis. Further it can promotes expression of ecto-59NT/CD73 and suppression of adenosine deaminase. It was observed that Daoy and ONS76 showed greater nuclear beta-catenin immunoreactivity than D283, which presented mainly cytoplasmic immunoreactivity. In summary, the absence of ecto59NT/CD73 in the D283 cell line, a metastatic MB phenotype, suggests that high expression levels of this ectonucleotidase could be correlated with a poor prognosis in patients with MB.application/pdfengPLoS ONE. San Francisco. Vol. 7, no. 10 (Oct. 2012), e47468, 11 f.5'-nucleotidaseCerebeloNeoplasiasMeduloblastomaCharacterization of ectonucleotidases in human medulloblastoma cell lines : ecto-5´NT/CD73 in metastasis as potential prognostic factorEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000876706.pdf.txt000876706.pdf.txtExtracted Texttext/plain51921http://www.lume.ufrgs.br/bitstream/10183/225259/2/000876706.pdf.txtefb16d1894cadfdaf238f22fccf315a2MD52ORIGINAL000876706.pdfTexto completo (inglês)application/pdf699976http://www.lume.ufrgs.br/bitstream/10183/225259/1/000876706.pdf251083aa21ffd010c4db74780bf53b1dMD5110183/2252592023-01-18 06:02:37.888766oai:www.lume.ufrgs.br:10183/225259Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-01-18T08:02:37Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Characterization of ectonucleotidases in human medulloblastoma cell lines : ecto-5´NT/CD73 in metastasis as potential prognostic factor |
title |
Characterization of ectonucleotidases in human medulloblastoma cell lines : ecto-5´NT/CD73 in metastasis as potential prognostic factor |
spellingShingle |
Characterization of ectonucleotidases in human medulloblastoma cell lines : ecto-5´NT/CD73 in metastasis as potential prognostic factor Cappellari, Angélica Regina 5'-nucleotidase Cerebelo Neoplasias Meduloblastoma |
title_short |
Characterization of ectonucleotidases in human medulloblastoma cell lines : ecto-5´NT/CD73 in metastasis as potential prognostic factor |
title_full |
Characterization of ectonucleotidases in human medulloblastoma cell lines : ecto-5´NT/CD73 in metastasis as potential prognostic factor |
title_fullStr |
Characterization of ectonucleotidases in human medulloblastoma cell lines : ecto-5´NT/CD73 in metastasis as potential prognostic factor |
title_full_unstemmed |
Characterization of ectonucleotidases in human medulloblastoma cell lines : ecto-5´NT/CD73 in metastasis as potential prognostic factor |
title_sort |
Characterization of ectonucleotidases in human medulloblastoma cell lines : ecto-5´NT/CD73 in metastasis as potential prognostic factor |
author |
Cappellari, Angélica Regina |
author_facet |
Cappellari, Angélica Regina Rockenbach, Liliana Dietrich, Fabrícia Clarimundo, Vanessa Schuck Glaser, Talita Braganhol, Elizandra Abujamra, Ana Lúcia Roesler, Rafael Ulrich, Henning Battastini, Ana Maria Oliveira |
author_role |
author |
author2 |
Rockenbach, Liliana Dietrich, Fabrícia Clarimundo, Vanessa Schuck Glaser, Talita Braganhol, Elizandra Abujamra, Ana Lúcia Roesler, Rafael Ulrich, Henning Battastini, Ana Maria Oliveira |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Cappellari, Angélica Regina Rockenbach, Liliana Dietrich, Fabrícia Clarimundo, Vanessa Schuck Glaser, Talita Braganhol, Elizandra Abujamra, Ana Lúcia Roesler, Rafael Ulrich, Henning Battastini, Ana Maria Oliveira |
dc.subject.por.fl_str_mv |
5'-nucleotidase Cerebelo Neoplasias Meduloblastoma |
topic |
5'-nucleotidase Cerebelo Neoplasias Meduloblastoma |
description |
Medulloblastoma (MB) is the most common malignant brain tumor in children and occurs mainly in the cerebellum. Important intracellular signaling molecules, such those present in the Sonic Hedgehog and Wnt pathways, are involved in its development and can also be employed to determine tumor grade and prognosis. Ectonucleotidases, particularly ecto59NT/CD73, are important enzymes in the malignant process of different tumor types regulating extracellular ATP and adenosine levels. Here, we investigated the activity of ectonucleotidases in three malignant human cell lines: Daoy and ONS76, being representative of primary MB, and the D283 cell line, derived from a metastatic MB. All cell lines secreted ATP into the extracellular medium while hydrolyze poorly this nucleotide, which is in agreement with the low expression and activity of pyrophosphate/phosphodiesterase, NTPDases and alkaline phosphatase. The analysis of AMP hydrolysis showed that Daoy and ONS76 completely hydrolyzed AMP, with parallel adenosine production (Daoy) and inosine accumulation (ONS76). On the other hand, D283 cell line did not hydrolyze AMP. Moreover, primary MB tumor cells, Daoy and ONS76 express the ecto-59NT/CD73 while D283 representative of a metastatic tumor, revealed poor expression of this enzyme, while the ecto-adenosine deaminase showed higher expression in D283 compared to Daoy and ONS76 cells. Nuclear betacatenin has been suggested as a marker for MB prognosis. Further it can promotes expression of ecto-59NT/CD73 and suppression of adenosine deaminase. It was observed that Daoy and ONS76 showed greater nuclear beta-catenin immunoreactivity than D283, which presented mainly cytoplasmic immunoreactivity. In summary, the absence of ecto59NT/CD73 in the D283 cell line, a metastatic MB phenotype, suggests that high expression levels of this ectonucleotidase could be correlated with a poor prognosis in patients with MB. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.accessioned.fl_str_mv |
2021-08-06T04:41:12Z |
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Estrangeiro info:eu-repo/semantics/article |
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http://hdl.handle.net/10183/225259 |
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1932-6203 |
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000876706 |
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dc.relation.ispartof.pt_BR.fl_str_mv |
PLoS ONE. San Francisco. Vol. 7, no. 10 (Oct. 2012), e47468, 11 f. |
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