Antiproliferative effect of colonic fermented phenolic compounds from jaboticaba (Myrciaria trunciflora) fruit peel in a 3D cell model of colorectal cancer
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/248391 |
Resumo: | Jaboticaba is a Brazilian native berry described as a rich source of phenolic compounds (PC) with health promoting effects. PC from jaboticaba peel powder (JPP) have low intestinal bio-accessibility and are catabolized by gut microbiota. However, the biological implication of PCderived metabolites produced during JPP digestion remains unclear. This study aimed to evaluate the antiproliferative effects of colonic fermented JPP (FJPP) in a 3D model of colorectal cancer (CRC) composed by HT29 spheroids. JPP samples fermented with human feces during 0, 2, 8, 24 or 48 h were incubated (10,000 µg mL−1 ) with spheroids, and cell viability was assessed after 72 h. Chemometric analyses (cluster and principal component analyses) were used to identify the main compounds responsible for the bioactive effect. The antiproliferative effect of FJPP in the CRC 3D model was increased between 8 h and 24 h of incubation, and this effect was associated with HHDP-digalloylglucose isomer and dihydroxyphenyl-γ-valerolactone. At 48 h of fermentation, the antiproliferative effect of FJPP was negligible, indicating that the presence of urolithins did not improve the bioactivity of JPP. These findings provide relevant knowledge on the role of colonic microbiota fermentation to generate active phenolic metabolites from JPP with positive impact on CRC. |
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Augusti, Paula RossiniQuatrin, AndréiaMello, ReniusBochi, Vivian CaetanoRodrigues, EliseuPrazeres, Inês D.Macedo, Ana CatarinaAlves, Sheila Cristina OliveiraEmanuelli, TatianaBronze, Maria do RosárioSerra, Ana Teresa2022-09-01T05:00:12Z20211420-3049http://hdl.handle.net/10183/248391001131354Jaboticaba is a Brazilian native berry described as a rich source of phenolic compounds (PC) with health promoting effects. PC from jaboticaba peel powder (JPP) have low intestinal bio-accessibility and are catabolized by gut microbiota. However, the biological implication of PCderived metabolites produced during JPP digestion remains unclear. This study aimed to evaluate the antiproliferative effects of colonic fermented JPP (FJPP) in a 3D model of colorectal cancer (CRC) composed by HT29 spheroids. JPP samples fermented with human feces during 0, 2, 8, 24 or 48 h were incubated (10,000 µg mL−1 ) with spheroids, and cell viability was assessed after 72 h. Chemometric analyses (cluster and principal component analyses) were used to identify the main compounds responsible for the bioactive effect. The antiproliferative effect of FJPP in the CRC 3D model was increased between 8 h and 24 h of incubation, and this effect was associated with HHDP-digalloylglucose isomer and dihydroxyphenyl-γ-valerolactone. At 48 h of fermentation, the antiproliferative effect of FJPP was negligible, indicating that the presence of urolithins did not improve the bioactivity of JPP. These findings provide relevant knowledge on the role of colonic microbiota fermentation to generate active phenolic metabolites from JPP with positive impact on CRC.application/pdfengMolecules. Basel. Vol. 26 (2021), 4469, 13 p.JabuticabaComposto fenólicoEsferoides celularesAnálise de clustersCâncer colorretalSpheroidsHT29 cellsCluster analysisPrincipal component analysisHydrolysable tanninsHHDP-digalloylglucose isomerDihydroxyphenyl-γ-valerolactoneAntiproliferative effect of colonic fermented phenolic compounds from jaboticaba (Myrciaria trunciflora) fruit peel in a 3D cell model of colorectal cancerEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001131354.pdf.txt001131354.pdf.txtExtracted Texttext/plain53745http://www.lume.ufrgs.br/bitstream/10183/248391/2/001131354.pdf.txte93beef0c07e0151a8dbaa1ac1a40616MD52ORIGINAL001131354.pdfTexto completo (inglês)application/pdf1962142http://www.lume.ufrgs.br/bitstream/10183/248391/1/001131354.pdfbaf7693f36672cd2afd08216e1677b44MD5110183/2483912022-09-02 04:59:48.674064oai:www.lume.ufrgs.br:10183/248391Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2022-09-02T07:59:48Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Antiproliferative effect of colonic fermented phenolic compounds from jaboticaba (Myrciaria trunciflora) fruit peel in a 3D cell model of colorectal cancer |
title |
Antiproliferative effect of colonic fermented phenolic compounds from jaboticaba (Myrciaria trunciflora) fruit peel in a 3D cell model of colorectal cancer |
spellingShingle |
Antiproliferative effect of colonic fermented phenolic compounds from jaboticaba (Myrciaria trunciflora) fruit peel in a 3D cell model of colorectal cancer Augusti, Paula Rossini Jabuticaba Composto fenólico Esferoides celulares Análise de clusters Câncer colorretal Spheroids HT29 cells Cluster analysis Principal component analysis Hydrolysable tannins HHDP-digalloylglucose isomer Dihydroxyphenyl-γ-valerolactone |
title_short |
Antiproliferative effect of colonic fermented phenolic compounds from jaboticaba (Myrciaria trunciflora) fruit peel in a 3D cell model of colorectal cancer |
title_full |
Antiproliferative effect of colonic fermented phenolic compounds from jaboticaba (Myrciaria trunciflora) fruit peel in a 3D cell model of colorectal cancer |
title_fullStr |
Antiproliferative effect of colonic fermented phenolic compounds from jaboticaba (Myrciaria trunciflora) fruit peel in a 3D cell model of colorectal cancer |
title_full_unstemmed |
Antiproliferative effect of colonic fermented phenolic compounds from jaboticaba (Myrciaria trunciflora) fruit peel in a 3D cell model of colorectal cancer |
title_sort |
Antiproliferative effect of colonic fermented phenolic compounds from jaboticaba (Myrciaria trunciflora) fruit peel in a 3D cell model of colorectal cancer |
author |
Augusti, Paula Rossini |
author_facet |
Augusti, Paula Rossini Quatrin, Andréia Mello, Renius Bochi, Vivian Caetano Rodrigues, Eliseu Prazeres, Inês D. Macedo, Ana Catarina Alves, Sheila Cristina Oliveira Emanuelli, Tatiana Bronze, Maria do Rosário Serra, Ana Teresa |
author_role |
author |
author2 |
Quatrin, Andréia Mello, Renius Bochi, Vivian Caetano Rodrigues, Eliseu Prazeres, Inês D. Macedo, Ana Catarina Alves, Sheila Cristina Oliveira Emanuelli, Tatiana Bronze, Maria do Rosário Serra, Ana Teresa |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Augusti, Paula Rossini Quatrin, Andréia Mello, Renius Bochi, Vivian Caetano Rodrigues, Eliseu Prazeres, Inês D. Macedo, Ana Catarina Alves, Sheila Cristina Oliveira Emanuelli, Tatiana Bronze, Maria do Rosário Serra, Ana Teresa |
dc.subject.por.fl_str_mv |
Jabuticaba Composto fenólico Esferoides celulares Análise de clusters Câncer colorretal |
topic |
Jabuticaba Composto fenólico Esferoides celulares Análise de clusters Câncer colorretal Spheroids HT29 cells Cluster analysis Principal component analysis Hydrolysable tannins HHDP-digalloylglucose isomer Dihydroxyphenyl-γ-valerolactone |
dc.subject.eng.fl_str_mv |
Spheroids HT29 cells Cluster analysis Principal component analysis Hydrolysable tannins HHDP-digalloylglucose isomer Dihydroxyphenyl-γ-valerolactone |
description |
Jaboticaba is a Brazilian native berry described as a rich source of phenolic compounds (PC) with health promoting effects. PC from jaboticaba peel powder (JPP) have low intestinal bio-accessibility and are catabolized by gut microbiota. However, the biological implication of PCderived metabolites produced during JPP digestion remains unclear. This study aimed to evaluate the antiproliferative effects of colonic fermented JPP (FJPP) in a 3D model of colorectal cancer (CRC) composed by HT29 spheroids. JPP samples fermented with human feces during 0, 2, 8, 24 or 48 h were incubated (10,000 µg mL−1 ) with spheroids, and cell viability was assessed after 72 h. Chemometric analyses (cluster and principal component analyses) were used to identify the main compounds responsible for the bioactive effect. The antiproliferative effect of FJPP in the CRC 3D model was increased between 8 h and 24 h of incubation, and this effect was associated with HHDP-digalloylglucose isomer and dihydroxyphenyl-γ-valerolactone. At 48 h of fermentation, the antiproliferative effect of FJPP was negligible, indicating that the presence of urolithins did not improve the bioactivity of JPP. These findings provide relevant knowledge on the role of colonic microbiota fermentation to generate active phenolic metabolites from JPP with positive impact on CRC. |
publishDate |
2021 |
dc.date.issued.fl_str_mv |
2021 |
dc.date.accessioned.fl_str_mv |
2022-09-01T05:00:12Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/248391 |
dc.identifier.issn.pt_BR.fl_str_mv |
1420-3049 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001131354 |
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1420-3049 001131354 |
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http://hdl.handle.net/10183/248391 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Molecules. Basel. Vol. 26 (2021), 4469, 13 p. |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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