Antiproliferative effect of colonic fermented phenolic compounds from jaboticaba (Myrciaria trunciflora) fruit peel in a 3D cell model of colorectal cancer

Detalhes bibliográficos
Autor(a) principal: Augusti, Paula Rossini
Data de Publicação: 2021
Outros Autores: Quatrin, Andréia, Mello, Renius, Bochi, Vivian Caetano, Rodrigues, Eliseu, Prazeres, Inês D., Macedo, Ana Catarina, Alves, Sheila Cristina Oliveira, Emanuelli, Tatiana, Bronze, Maria do Rosário, Serra, Ana Teresa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/248391
Resumo: Jaboticaba is a Brazilian native berry described as a rich source of phenolic compounds (PC) with health promoting effects. PC from jaboticaba peel powder (JPP) have low intestinal bio-accessibility and are catabolized by gut microbiota. However, the biological implication of PCderived metabolites produced during JPP digestion remains unclear. This study aimed to evaluate the antiproliferative effects of colonic fermented JPP (FJPP) in a 3D model of colorectal cancer (CRC) composed by HT29 spheroids. JPP samples fermented with human feces during 0, 2, 8, 24 or 48 h were incubated (10,000 µg mL−1 ) with spheroids, and cell viability was assessed after 72 h. Chemometric analyses (cluster and principal component analyses) were used to identify the main compounds responsible for the bioactive effect. The antiproliferative effect of FJPP in the CRC 3D model was increased between 8 h and 24 h of incubation, and this effect was associated with HHDP-digalloylglucose isomer and dihydroxyphenyl-γ-valerolactone. At 48 h of fermentation, the antiproliferative effect of FJPP was negligible, indicating that the presence of urolithins did not improve the bioactivity of JPP. These findings provide relevant knowledge on the role of colonic microbiota fermentation to generate active phenolic metabolites from JPP with positive impact on CRC.
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spelling Augusti, Paula RossiniQuatrin, AndréiaMello, ReniusBochi, Vivian CaetanoRodrigues, EliseuPrazeres, Inês D.Macedo, Ana CatarinaAlves, Sheila Cristina OliveiraEmanuelli, TatianaBronze, Maria do RosárioSerra, Ana Teresa2022-09-01T05:00:12Z20211420-3049http://hdl.handle.net/10183/248391001131354Jaboticaba is a Brazilian native berry described as a rich source of phenolic compounds (PC) with health promoting effects. PC from jaboticaba peel powder (JPP) have low intestinal bio-accessibility and are catabolized by gut microbiota. However, the biological implication of PCderived metabolites produced during JPP digestion remains unclear. This study aimed to evaluate the antiproliferative effects of colonic fermented JPP (FJPP) in a 3D model of colorectal cancer (CRC) composed by HT29 spheroids. JPP samples fermented with human feces during 0, 2, 8, 24 or 48 h were incubated (10,000 µg mL−1 ) with spheroids, and cell viability was assessed after 72 h. Chemometric analyses (cluster and principal component analyses) were used to identify the main compounds responsible for the bioactive effect. The antiproliferative effect of FJPP in the CRC 3D model was increased between 8 h and 24 h of incubation, and this effect was associated with HHDP-digalloylglucose isomer and dihydroxyphenyl-γ-valerolactone. At 48 h of fermentation, the antiproliferative effect of FJPP was negligible, indicating that the presence of urolithins did not improve the bioactivity of JPP. These findings provide relevant knowledge on the role of colonic microbiota fermentation to generate active phenolic metabolites from JPP with positive impact on CRC.application/pdfengMolecules. Basel. Vol. 26 (2021), 4469, 13 p.JabuticabaComposto fenólicoEsferoides celularesAnálise de clustersCâncer colorretalSpheroidsHT29 cellsCluster analysisPrincipal component analysisHydrolysable tanninsHHDP-digalloylglucose isomerDihydroxyphenyl-γ-valerolactoneAntiproliferative effect of colonic fermented phenolic compounds from jaboticaba (Myrciaria trunciflora) fruit peel in a 3D cell model of colorectal cancerEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001131354.pdf.txt001131354.pdf.txtExtracted Texttext/plain53745http://www.lume.ufrgs.br/bitstream/10183/248391/2/001131354.pdf.txte93beef0c07e0151a8dbaa1ac1a40616MD52ORIGINAL001131354.pdfTexto completo (inglês)application/pdf1962142http://www.lume.ufrgs.br/bitstream/10183/248391/1/001131354.pdfbaf7693f36672cd2afd08216e1677b44MD5110183/2483912022-09-02 04:59:48.674064oai:www.lume.ufrgs.br:10183/248391Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2022-09-02T07:59:48Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Antiproliferative effect of colonic fermented phenolic compounds from jaboticaba (Myrciaria trunciflora) fruit peel in a 3D cell model of colorectal cancer
title Antiproliferative effect of colonic fermented phenolic compounds from jaboticaba (Myrciaria trunciflora) fruit peel in a 3D cell model of colorectal cancer
spellingShingle Antiproliferative effect of colonic fermented phenolic compounds from jaboticaba (Myrciaria trunciflora) fruit peel in a 3D cell model of colorectal cancer
Augusti, Paula Rossini
Jabuticaba
Composto fenólico
Esferoides celulares
Análise de clusters
Câncer colorretal
Spheroids
HT29 cells
Cluster analysis
Principal component analysis
Hydrolysable tannins
HHDP-digalloylglucose isomer
Dihydroxyphenyl-γ-valerolactone
title_short Antiproliferative effect of colonic fermented phenolic compounds from jaboticaba (Myrciaria trunciflora) fruit peel in a 3D cell model of colorectal cancer
title_full Antiproliferative effect of colonic fermented phenolic compounds from jaboticaba (Myrciaria trunciflora) fruit peel in a 3D cell model of colorectal cancer
title_fullStr Antiproliferative effect of colonic fermented phenolic compounds from jaboticaba (Myrciaria trunciflora) fruit peel in a 3D cell model of colorectal cancer
title_full_unstemmed Antiproliferative effect of colonic fermented phenolic compounds from jaboticaba (Myrciaria trunciflora) fruit peel in a 3D cell model of colorectal cancer
title_sort Antiproliferative effect of colonic fermented phenolic compounds from jaboticaba (Myrciaria trunciflora) fruit peel in a 3D cell model of colorectal cancer
author Augusti, Paula Rossini
author_facet Augusti, Paula Rossini
Quatrin, Andréia
Mello, Renius
Bochi, Vivian Caetano
Rodrigues, Eliseu
Prazeres, Inês D.
Macedo, Ana Catarina
Alves, Sheila Cristina Oliveira
Emanuelli, Tatiana
Bronze, Maria do Rosário
Serra, Ana Teresa
author_role author
author2 Quatrin, Andréia
Mello, Renius
Bochi, Vivian Caetano
Rodrigues, Eliseu
Prazeres, Inês D.
Macedo, Ana Catarina
Alves, Sheila Cristina Oliveira
Emanuelli, Tatiana
Bronze, Maria do Rosário
Serra, Ana Teresa
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Augusti, Paula Rossini
Quatrin, Andréia
Mello, Renius
Bochi, Vivian Caetano
Rodrigues, Eliseu
Prazeres, Inês D.
Macedo, Ana Catarina
Alves, Sheila Cristina Oliveira
Emanuelli, Tatiana
Bronze, Maria do Rosário
Serra, Ana Teresa
dc.subject.por.fl_str_mv Jabuticaba
Composto fenólico
Esferoides celulares
Análise de clusters
Câncer colorretal
topic Jabuticaba
Composto fenólico
Esferoides celulares
Análise de clusters
Câncer colorretal
Spheroids
HT29 cells
Cluster analysis
Principal component analysis
Hydrolysable tannins
HHDP-digalloylglucose isomer
Dihydroxyphenyl-γ-valerolactone
dc.subject.eng.fl_str_mv Spheroids
HT29 cells
Cluster analysis
Principal component analysis
Hydrolysable tannins
HHDP-digalloylglucose isomer
Dihydroxyphenyl-γ-valerolactone
description Jaboticaba is a Brazilian native berry described as a rich source of phenolic compounds (PC) with health promoting effects. PC from jaboticaba peel powder (JPP) have low intestinal bio-accessibility and are catabolized by gut microbiota. However, the biological implication of PCderived metabolites produced during JPP digestion remains unclear. This study aimed to evaluate the antiproliferative effects of colonic fermented JPP (FJPP) in a 3D model of colorectal cancer (CRC) composed by HT29 spheroids. JPP samples fermented with human feces during 0, 2, 8, 24 or 48 h were incubated (10,000 µg mL−1 ) with spheroids, and cell viability was assessed after 72 h. Chemometric analyses (cluster and principal component analyses) were used to identify the main compounds responsible for the bioactive effect. The antiproliferative effect of FJPP in the CRC 3D model was increased between 8 h and 24 h of incubation, and this effect was associated with HHDP-digalloylglucose isomer and dihydroxyphenyl-γ-valerolactone. At 48 h of fermentation, the antiproliferative effect of FJPP was negligible, indicating that the presence of urolithins did not improve the bioactivity of JPP. These findings provide relevant knowledge on the role of colonic microbiota fermentation to generate active phenolic metabolites from JPP with positive impact on CRC.
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2022-09-01T05:00:12Z
dc.type.driver.fl_str_mv Estrangeiro
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/248391
dc.identifier.issn.pt_BR.fl_str_mv 1420-3049
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dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Molecules. Basel. Vol. 26 (2021), 4469, 13 p.
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