Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells

Detalhes bibliográficos
Autor(a) principal: Horbach, Leonardo
Data de Publicação: 2018
Outros Autores: Sinigaglia, Marialva, Silva, Camila Alves da, Olguins, Danielly Brufatto, Gregianin, Lauro José, Brunetto, Algemir Lunardi, Brunetto, Andre Tessainer, Roesler, Rafael, Farias, Caroline Brunetto de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/188690
Resumo: Ewing Sarcoma (ES) is a highly aggressive bone and soft tissue childhood cancer. The development of resistance to chemotherapy is common and remains the main cause of treatment failure. We herein evaluated the expression of genes associated with chemotherapy resistance in ES cell lines. A set of genes (CCAR1, TUBA1A, POLDIP2, SMARCA4 and SMARCB1) was data-mined for resistance against doxorubicin and vincristine, which are the standard drugs used in the treatment of patients with ES. The expression of each gene in SK-ES-1 ES cells was reported before and after exposure to a drug resistance-inducing protocol. There was a significant downregulation of CCAR1 and TUBA1A in doxorubicin-resistant cells, with low expression of TUBA1A in vincristine-resistant cells. By contrast, POLDIP2 was significantly upregulated in cells resistant to either drug, and the expression of the SMARCB1 and SMARCA4 genes was upregulated in doxorubicin-resistant cells. These findings indicate that resistance to specific chemotherapeutic agents was accompanied by differential changes in gene expression in ES tumors.
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spelling Horbach, LeonardoSinigaglia, MarialvaSilva, Camila Alves daOlguins, Danielly BrufattoGregianin, Lauro JoséBrunetto, Algemir LunardiBrunetto, Andre TessainerRoesler, RafaelFarias, Caroline Brunetto de2019-02-12T02:33:32Z20182049-9469http://hdl.handle.net/10183/188690001069971Ewing Sarcoma (ES) is a highly aggressive bone and soft tissue childhood cancer. The development of resistance to chemotherapy is common and remains the main cause of treatment failure. We herein evaluated the expression of genes associated with chemotherapy resistance in ES cell lines. A set of genes (CCAR1, TUBA1A, POLDIP2, SMARCA4 and SMARCB1) was data-mined for resistance against doxorubicin and vincristine, which are the standard drugs used in the treatment of patients with ES. The expression of each gene in SK-ES-1 ES cells was reported before and after exposure to a drug resistance-inducing protocol. There was a significant downregulation of CCAR1 and TUBA1A in doxorubicin-resistant cells, with low expression of TUBA1A in vincristine-resistant cells. By contrast, POLDIP2 was significantly upregulated in cells resistant to either drug, and the expression of the SMARCB1 and SMARCA4 genes was upregulated in doxorubicin-resistant cells. These findings indicate that resistance to specific chemotherapeutic agents was accompanied by differential changes in gene expression in ES tumors.application/pdfengMolecular and clinical oncology. Londres. Vol. 9, n. 6 (June 2018), p. 719-724Sarcoma de EwingExpressão gênicaResistência a medicamentosDoxorrubicinaVincristinaGene expression changes associated with chemotherapy resistance in Ewing sarcoma cellsEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001069971.pdf.txt001069971.pdf.txtExtracted Texttext/plain26443http://www.lume.ufrgs.br/bitstream/10183/188690/2/001069971.pdf.txt18656085ca3ba93688b6d2619474e30eMD52ORIGINAL001069971.pdfTexto completo (inglês)application/pdf877091http://www.lume.ufrgs.br/bitstream/10183/188690/1/001069971.pdfca8ae0cc5efa8c52e99ae03ef77f8cacMD5110183/1886902023-04-21 03:23:24.722177oai:www.lume.ufrgs.br:10183/188690Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-04-21T06:23:24Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells
title Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells
spellingShingle Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells
Horbach, Leonardo
Sarcoma de Ewing
Expressão gênica
Resistência a medicamentos
Doxorrubicina
Vincristina
title_short Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells
title_full Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells
title_fullStr Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells
title_full_unstemmed Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells
title_sort Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells
author Horbach, Leonardo
author_facet Horbach, Leonardo
Sinigaglia, Marialva
Silva, Camila Alves da
Olguins, Danielly Brufatto
Gregianin, Lauro José
Brunetto, Algemir Lunardi
Brunetto, Andre Tessainer
Roesler, Rafael
Farias, Caroline Brunetto de
author_role author
author2 Sinigaglia, Marialva
Silva, Camila Alves da
Olguins, Danielly Brufatto
Gregianin, Lauro José
Brunetto, Algemir Lunardi
Brunetto, Andre Tessainer
Roesler, Rafael
Farias, Caroline Brunetto de
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Horbach, Leonardo
Sinigaglia, Marialva
Silva, Camila Alves da
Olguins, Danielly Brufatto
Gregianin, Lauro José
Brunetto, Algemir Lunardi
Brunetto, Andre Tessainer
Roesler, Rafael
Farias, Caroline Brunetto de
dc.subject.por.fl_str_mv Sarcoma de Ewing
Expressão gênica
Resistência a medicamentos
Doxorrubicina
Vincristina
topic Sarcoma de Ewing
Expressão gênica
Resistência a medicamentos
Doxorrubicina
Vincristina
description Ewing Sarcoma (ES) is a highly aggressive bone and soft tissue childhood cancer. The development of resistance to chemotherapy is common and remains the main cause of treatment failure. We herein evaluated the expression of genes associated with chemotherapy resistance in ES cell lines. A set of genes (CCAR1, TUBA1A, POLDIP2, SMARCA4 and SMARCB1) was data-mined for resistance against doxorubicin and vincristine, which are the standard drugs used in the treatment of patients with ES. The expression of each gene in SK-ES-1 ES cells was reported before and after exposure to a drug resistance-inducing protocol. There was a significant downregulation of CCAR1 and TUBA1A in doxorubicin-resistant cells, with low expression of TUBA1A in vincristine-resistant cells. By contrast, POLDIP2 was significantly upregulated in cells resistant to either drug, and the expression of the SMARCB1 and SMARCA4 genes was upregulated in doxorubicin-resistant cells. These findings indicate that resistance to specific chemotherapeutic agents was accompanied by differential changes in gene expression in ES tumors.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-02-12T02:33:32Z
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dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Molecular and clinical oncology. Londres. Vol. 9, n. 6 (June 2018), p. 719-724
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