Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/188690 |
Resumo: | Ewing Sarcoma (ES) is a highly aggressive bone and soft tissue childhood cancer. The development of resistance to chemotherapy is common and remains the main cause of treatment failure. We herein evaluated the expression of genes associated with chemotherapy resistance in ES cell lines. A set of genes (CCAR1, TUBA1A, POLDIP2, SMARCA4 and SMARCB1) was data-mined for resistance against doxorubicin and vincristine, which are the standard drugs used in the treatment of patients with ES. The expression of each gene in SK-ES-1 ES cells was reported before and after exposure to a drug resistance-inducing protocol. There was a significant downregulation of CCAR1 and TUBA1A in doxorubicin-resistant cells, with low expression of TUBA1A in vincristine-resistant cells. By contrast, POLDIP2 was significantly upregulated in cells resistant to either drug, and the expression of the SMARCB1 and SMARCA4 genes was upregulated in doxorubicin-resistant cells. These findings indicate that resistance to specific chemotherapeutic agents was accompanied by differential changes in gene expression in ES tumors. |
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Horbach, LeonardoSinigaglia, MarialvaSilva, Camila Alves daOlguins, Danielly BrufattoGregianin, Lauro JoséBrunetto, Algemir LunardiBrunetto, Andre TessainerRoesler, RafaelFarias, Caroline Brunetto de2019-02-12T02:33:32Z20182049-9469http://hdl.handle.net/10183/188690001069971Ewing Sarcoma (ES) is a highly aggressive bone and soft tissue childhood cancer. The development of resistance to chemotherapy is common and remains the main cause of treatment failure. We herein evaluated the expression of genes associated with chemotherapy resistance in ES cell lines. A set of genes (CCAR1, TUBA1A, POLDIP2, SMARCA4 and SMARCB1) was data-mined for resistance against doxorubicin and vincristine, which are the standard drugs used in the treatment of patients with ES. The expression of each gene in SK-ES-1 ES cells was reported before and after exposure to a drug resistance-inducing protocol. There was a significant downregulation of CCAR1 and TUBA1A in doxorubicin-resistant cells, with low expression of TUBA1A in vincristine-resistant cells. By contrast, POLDIP2 was significantly upregulated in cells resistant to either drug, and the expression of the SMARCB1 and SMARCA4 genes was upregulated in doxorubicin-resistant cells. These findings indicate that resistance to specific chemotherapeutic agents was accompanied by differential changes in gene expression in ES tumors.application/pdfengMolecular and clinical oncology. Londres. Vol. 9, n. 6 (June 2018), p. 719-724Sarcoma de EwingExpressão gênicaResistência a medicamentosDoxorrubicinaVincristinaGene expression changes associated with chemotherapy resistance in Ewing sarcoma cellsEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001069971.pdf.txt001069971.pdf.txtExtracted Texttext/plain26443http://www.lume.ufrgs.br/bitstream/10183/188690/2/001069971.pdf.txt18656085ca3ba93688b6d2619474e30eMD52ORIGINAL001069971.pdfTexto completo (inglês)application/pdf877091http://www.lume.ufrgs.br/bitstream/10183/188690/1/001069971.pdfca8ae0cc5efa8c52e99ae03ef77f8cacMD5110183/1886902023-04-21 03:23:24.722177oai:www.lume.ufrgs.br:10183/188690Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-04-21T06:23:24Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells |
title |
Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells |
spellingShingle |
Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells Horbach, Leonardo Sarcoma de Ewing Expressão gênica Resistência a medicamentos Doxorrubicina Vincristina |
title_short |
Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells |
title_full |
Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells |
title_fullStr |
Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells |
title_full_unstemmed |
Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells |
title_sort |
Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells |
author |
Horbach, Leonardo |
author_facet |
Horbach, Leonardo Sinigaglia, Marialva Silva, Camila Alves da Olguins, Danielly Brufatto Gregianin, Lauro José Brunetto, Algemir Lunardi Brunetto, Andre Tessainer Roesler, Rafael Farias, Caroline Brunetto de |
author_role |
author |
author2 |
Sinigaglia, Marialva Silva, Camila Alves da Olguins, Danielly Brufatto Gregianin, Lauro José Brunetto, Algemir Lunardi Brunetto, Andre Tessainer Roesler, Rafael Farias, Caroline Brunetto de |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Horbach, Leonardo Sinigaglia, Marialva Silva, Camila Alves da Olguins, Danielly Brufatto Gregianin, Lauro José Brunetto, Algemir Lunardi Brunetto, Andre Tessainer Roesler, Rafael Farias, Caroline Brunetto de |
dc.subject.por.fl_str_mv |
Sarcoma de Ewing Expressão gênica Resistência a medicamentos Doxorrubicina Vincristina |
topic |
Sarcoma de Ewing Expressão gênica Resistência a medicamentos Doxorrubicina Vincristina |
description |
Ewing Sarcoma (ES) is a highly aggressive bone and soft tissue childhood cancer. The development of resistance to chemotherapy is common and remains the main cause of treatment failure. We herein evaluated the expression of genes associated with chemotherapy resistance in ES cell lines. A set of genes (CCAR1, TUBA1A, POLDIP2, SMARCA4 and SMARCB1) was data-mined for resistance against doxorubicin and vincristine, which are the standard drugs used in the treatment of patients with ES. The expression of each gene in SK-ES-1 ES cells was reported before and after exposure to a drug resistance-inducing protocol. There was a significant downregulation of CCAR1 and TUBA1A in doxorubicin-resistant cells, with low expression of TUBA1A in vincristine-resistant cells. By contrast, POLDIP2 was significantly upregulated in cells resistant to either drug, and the expression of the SMARCB1 and SMARCA4 genes was upregulated in doxorubicin-resistant cells. These findings indicate that resistance to specific chemotherapeutic agents was accompanied by differential changes in gene expression in ES tumors. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2019-02-12T02:33:32Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/188690 |
dc.identifier.issn.pt_BR.fl_str_mv |
2049-9469 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001069971 |
identifier_str_mv |
2049-9469 001069971 |
url |
http://hdl.handle.net/10183/188690 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Molecular and clinical oncology. Londres. Vol. 9, n. 6 (June 2018), p. 719-724 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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