Anti-inflammatory activity and chemical analysis of extracts from Trifolium riograndense
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/178549 |
Resumo: | Aiming to investigate new therapeutic agents with fewer side effects, the number of studies about naturalproducts has increased. Phenolic compounds comprise a well-studied class of abundant plant-derivedcompounds, whose anti-inflammatory activity has been described. Isoflavones are phenolic compoundsthat occur mainly in the Leguminosae family, and can be found in many species, such as Trifoliumriograndense Burkart, Leguminosae (clover). In this study an HPLC method was used to determine andquantify four isoflavones (genistein, daidzein, formononetin, and biochanin A) in hydrolyzed leaf, flower,stolon, and root extracts of T. riograndense. In vivo anti-inflammatory activity was investigated using therat paw edema method and in vitro chemotaxis model with a dry extract from the leaves, which had thehighest amount of isoflavones. The major isoflavone found in all parts of the plant was formononetin.The chemotaxis assay revealed that the different concentrations (0.2–50 g/ml) of the dry extract sig-nificantly inhibited neutrophil migration in a concentration-dependent manner (more than 90%). In therat paw edema test, oral administration of clover extract 100 mg/kg was able to significantly inhibitthe edema formation induced by carrageenan. In conclusion, chemical analyses showed that Trifoliumriograndense is a plant rich in isoflavones and a new interesting option as isoflavone source. The results ofthe biological tests taken together show that the extract of T. riograndense has anti-inflammatory effectin rodents. |
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Pedrazza, Graziele Pereira RamosMorais, Cláudia Borges deDettenborn, Greice RaquelCeolato, Paula CasagrandeApel, Miriam AndersSchapoval, Elfrides Eva SchermanDall'Agnol, MiguelZuanazzi, Jose Angelo Silveira2018-05-18T02:28:10Z20170102-695Xhttp://hdl.handle.net/10183/178549001067797Aiming to investigate new therapeutic agents with fewer side effects, the number of studies about naturalproducts has increased. Phenolic compounds comprise a well-studied class of abundant plant-derivedcompounds, whose anti-inflammatory activity has been described. Isoflavones are phenolic compoundsthat occur mainly in the Leguminosae family, and can be found in many species, such as Trifoliumriograndense Burkart, Leguminosae (clover). In this study an HPLC method was used to determine andquantify four isoflavones (genistein, daidzein, formononetin, and biochanin A) in hydrolyzed leaf, flower,stolon, and root extracts of T. riograndense. In vivo anti-inflammatory activity was investigated using therat paw edema method and in vitro chemotaxis model with a dry extract from the leaves, which had thehighest amount of isoflavones. The major isoflavone found in all parts of the plant was formononetin.The chemotaxis assay revealed that the different concentrations (0.2–50 g/ml) of the dry extract sig-nificantly inhibited neutrophil migration in a concentration-dependent manner (more than 90%). In therat paw edema test, oral administration of clover extract 100 mg/kg was able to significantly inhibitthe edema formation induced by carrageenan. In conclusion, chemical analyses showed that Trifoliumriograndense is a plant rich in isoflavones and a new interesting option as isoflavone source. The results ofthe biological tests taken together show that the extract of T. riograndense has anti-inflammatory effectin rodents.application/pdfengRevista brasileira de farmacognosia. Vol. 27, no. 3 (May/June 2017), p. 334-338Trifolium riograndenseAntiinflamatoriosHPLCIsoflavonesLeguminosaeNeutrophil chemotaxisRat paw edemaAnti-inflammatory activity and chemical analysis of extracts from Trifolium riograndenseinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001067797.pdf001067797.pdfTexto completo (inglês)application/pdf614687http://www.lume.ufrgs.br/bitstream/10183/178549/1/001067797.pdf345631aa554f3f9eb5e8f4ad5f5e1b16MD51TEXT001067797.pdf.txt001067797.pdf.txtExtracted Texttext/plain32468http://www.lume.ufrgs.br/bitstream/10183/178549/2/001067797.pdf.txt3909c0ff42bd3568f85d08dc64763e28MD5210183/1785492018-05-19 03:18:22.194599oai:www.lume.ufrgs.br:10183/178549Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-05-19T06:18:22Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Anti-inflammatory activity and chemical analysis of extracts from Trifolium riograndense |
title |
Anti-inflammatory activity and chemical analysis of extracts from Trifolium riograndense |
spellingShingle |
Anti-inflammatory activity and chemical analysis of extracts from Trifolium riograndense Pedrazza, Graziele Pereira Ramos Trifolium riograndense Antiinflamatorios HPLC Isoflavones Leguminosae Neutrophil chemotaxis Rat paw edema |
title_short |
Anti-inflammatory activity and chemical analysis of extracts from Trifolium riograndense |
title_full |
Anti-inflammatory activity and chemical analysis of extracts from Trifolium riograndense |
title_fullStr |
Anti-inflammatory activity and chemical analysis of extracts from Trifolium riograndense |
title_full_unstemmed |
Anti-inflammatory activity and chemical analysis of extracts from Trifolium riograndense |
title_sort |
Anti-inflammatory activity and chemical analysis of extracts from Trifolium riograndense |
author |
Pedrazza, Graziele Pereira Ramos |
author_facet |
Pedrazza, Graziele Pereira Ramos Morais, Cláudia Borges de Dettenborn, Greice Raquel Ceolato, Paula Casagrande Apel, Miriam Anders Schapoval, Elfrides Eva Scherman Dall'Agnol, Miguel Zuanazzi, Jose Angelo Silveira |
author_role |
author |
author2 |
Morais, Cláudia Borges de Dettenborn, Greice Raquel Ceolato, Paula Casagrande Apel, Miriam Anders Schapoval, Elfrides Eva Scherman Dall'Agnol, Miguel Zuanazzi, Jose Angelo Silveira |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Pedrazza, Graziele Pereira Ramos Morais, Cláudia Borges de Dettenborn, Greice Raquel Ceolato, Paula Casagrande Apel, Miriam Anders Schapoval, Elfrides Eva Scherman Dall'Agnol, Miguel Zuanazzi, Jose Angelo Silveira |
dc.subject.por.fl_str_mv |
Trifolium riograndense Antiinflamatorios |
topic |
Trifolium riograndense Antiinflamatorios HPLC Isoflavones Leguminosae Neutrophil chemotaxis Rat paw edema |
dc.subject.eng.fl_str_mv |
HPLC Isoflavones Leguminosae Neutrophil chemotaxis Rat paw edema |
description |
Aiming to investigate new therapeutic agents with fewer side effects, the number of studies about naturalproducts has increased. Phenolic compounds comprise a well-studied class of abundant plant-derivedcompounds, whose anti-inflammatory activity has been described. Isoflavones are phenolic compoundsthat occur mainly in the Leguminosae family, and can be found in many species, such as Trifoliumriograndense Burkart, Leguminosae (clover). In this study an HPLC method was used to determine andquantify four isoflavones (genistein, daidzein, formononetin, and biochanin A) in hydrolyzed leaf, flower,stolon, and root extracts of T. riograndense. In vivo anti-inflammatory activity was investigated using therat paw edema method and in vitro chemotaxis model with a dry extract from the leaves, which had thehighest amount of isoflavones. The major isoflavone found in all parts of the plant was formononetin.The chemotaxis assay revealed that the different concentrations (0.2–50 g/ml) of the dry extract sig-nificantly inhibited neutrophil migration in a concentration-dependent manner (more than 90%). In therat paw edema test, oral administration of clover extract 100 mg/kg was able to significantly inhibitthe edema formation induced by carrageenan. In conclusion, chemical analyses showed that Trifoliumriograndense is a plant rich in isoflavones and a new interesting option as isoflavone source. The results ofthe biological tests taken together show that the extract of T. riograndense has anti-inflammatory effectin rodents. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2018-05-18T02:28:10Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/178549 |
dc.identifier.issn.pt_BR.fl_str_mv |
0102-695X |
dc.identifier.nrb.pt_BR.fl_str_mv |
001067797 |
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0102-695X 001067797 |
url |
http://hdl.handle.net/10183/178549 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Revista brasileira de farmacognosia. Vol. 27, no. 3 (May/June 2017), p. 334-338 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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