2-(Dimethylamino)ethyl methacrylate/(2-hydroxyethyl) methacrylate/α-tricalcium phosphate cryogels for bone repair, preparation and evaluation of the biological response of human trabecular bone-derived cells and mesenchymal stem cells

Detalhes bibliográficos
Autor(a) principal: Volkmer, Tiago Moreno
Data de Publicação: 2014
Outros Autores: Magalhães, Joana Cristina Silva, Sousa, Vânia Caldas de, Santos, Luis Alberto dos, Burguera, Elena Fernández, Blanco Garcíaz, Francisco Javier, San Román, Julio, Rodríguez-Lorenzo, Luis M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/267620
Resumo: The aim of this work is to evaluate the potential of cryogels to be used as scaffolds in tissue engineering. Scaffolds based on the α-tricalcium phosphate reinforced PDMAEMA (Poly(dimethyl aminoethyl methacrylate))/PHEMA (poly(hydroxyethyl methacrylate)) system were prepared and human trabecular bone-derived cells (HTBs) and bone marrow derived-mesenchymal stem cells (BM-MSCs) cultured on them. Several features, such as porosity, pore shape, molecular weight between crosslinks and mesh size, are studied. The most suitable PDMAEMA/PHEMA ratio for cell proliferation has been assessed and the viability, adhesion, proliferation and expression of osteoblastic biochemical markers are evaluated. The PDMAEMA/PHEMA ratio influences the scaffolds porosity. Values between 53% ± 5.7% for a greater content in PHEMA and 75% ± 5.5% for a greater content in PDMAEMA have been obtained. The polymer ratio also modifies the pore shape. A greater content in PDMAEMA leads also to bigger network mesh size. Each of the compositions were non-cytotoxic, the seeded cells remained viable for both BM-MSCs and HTBs. Thus, and based on the structural analysis, specimens with a greater content in PDMAEMA seem to provide a better structural environment for their use as scaffolds for tissue engineering. The α-tricalcium phosphate incorporation into the composition seems to favor the expression of the osteogenic phenotype.
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spelling Volkmer, Tiago MorenoMagalhães, Joana Cristina SilvaSousa, Vânia Caldas deSantos, Luis Alberto dosBurguera, Elena FernándezBlanco Garcíaz, Francisco JavierSan Román, JulioRodríguez-Lorenzo, Luis M.2023-11-25T03:26:19Z20142073-4360http://hdl.handle.net/10183/267620000949609The aim of this work is to evaluate the potential of cryogels to be used as scaffolds in tissue engineering. Scaffolds based on the α-tricalcium phosphate reinforced PDMAEMA (Poly(dimethyl aminoethyl methacrylate))/PHEMA (poly(hydroxyethyl methacrylate)) system were prepared and human trabecular bone-derived cells (HTBs) and bone marrow derived-mesenchymal stem cells (BM-MSCs) cultured on them. Several features, such as porosity, pore shape, molecular weight between crosslinks and mesh size, are studied. The most suitable PDMAEMA/PHEMA ratio for cell proliferation has been assessed and the viability, adhesion, proliferation and expression of osteoblastic biochemical markers are evaluated. The PDMAEMA/PHEMA ratio influences the scaffolds porosity. Values between 53% ± 5.7% for a greater content in PHEMA and 75% ± 5.5% for a greater content in PDMAEMA have been obtained. The polymer ratio also modifies the pore shape. A greater content in PDMAEMA leads also to bigger network mesh size. Each of the compositions were non-cytotoxic, the seeded cells remained viable for both BM-MSCs and HTBs. Thus, and based on the structural analysis, specimens with a greater content in PDMAEMA seem to provide a better structural environment for their use as scaffolds for tissue engineering. The α-tricalcium phosphate incorporation into the composition seems to favor the expression of the osteogenic phenotype.application/pdfengPolymers [recurso eletrônico]. Basel. Vol. 6 (2014), p. 2510-2525BiomateriaisEngenharia de tecidosBiopolímerosCryogelsα-tricalcium phosphateMesenchymal stem cells2-(Dimethylamino)ethyl methacrylate/(2-hydroxyethyl) methacrylate/α-tricalcium phosphate cryogels for bone repair, preparation and evaluation of the biological response of human trabecular bone-derived cells and mesenchymal stem cellsEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000949609.pdf.txt000949609.pdf.txtExtracted Texttext/plain37047http://www.lume.ufrgs.br/bitstream/10183/267620/2/000949609.pdf.txt5f117b0482b174ef383161c336561595MD52ORIGINAL000949609.pdfTexto completo (inglês)application/pdf6291553http://www.lume.ufrgs.br/bitstream/10183/267620/1/000949609.pdfaace0f69d80723a33aee700371153a52MD5110183/2676202023-12-06 04:24:41.974755oai:www.lume.ufrgs.br:10183/267620Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-12-06T06:24:41Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv 2-(Dimethylamino)ethyl methacrylate/(2-hydroxyethyl) methacrylate/α-tricalcium phosphate cryogels for bone repair, preparation and evaluation of the biological response of human trabecular bone-derived cells and mesenchymal stem cells
title 2-(Dimethylamino)ethyl methacrylate/(2-hydroxyethyl) methacrylate/α-tricalcium phosphate cryogels for bone repair, preparation and evaluation of the biological response of human trabecular bone-derived cells and mesenchymal stem cells
spellingShingle 2-(Dimethylamino)ethyl methacrylate/(2-hydroxyethyl) methacrylate/α-tricalcium phosphate cryogels for bone repair, preparation and evaluation of the biological response of human trabecular bone-derived cells and mesenchymal stem cells
Volkmer, Tiago Moreno
Biomateriais
Engenharia de tecidos
Biopolímeros
Cryogels
α-tricalcium phosphate
Mesenchymal stem cells
title_short 2-(Dimethylamino)ethyl methacrylate/(2-hydroxyethyl) methacrylate/α-tricalcium phosphate cryogels for bone repair, preparation and evaluation of the biological response of human trabecular bone-derived cells and mesenchymal stem cells
title_full 2-(Dimethylamino)ethyl methacrylate/(2-hydroxyethyl) methacrylate/α-tricalcium phosphate cryogels for bone repair, preparation and evaluation of the biological response of human trabecular bone-derived cells and mesenchymal stem cells
title_fullStr 2-(Dimethylamino)ethyl methacrylate/(2-hydroxyethyl) methacrylate/α-tricalcium phosphate cryogels for bone repair, preparation and evaluation of the biological response of human trabecular bone-derived cells and mesenchymal stem cells
title_full_unstemmed 2-(Dimethylamino)ethyl methacrylate/(2-hydroxyethyl) methacrylate/α-tricalcium phosphate cryogels for bone repair, preparation and evaluation of the biological response of human trabecular bone-derived cells and mesenchymal stem cells
title_sort 2-(Dimethylamino)ethyl methacrylate/(2-hydroxyethyl) methacrylate/α-tricalcium phosphate cryogels for bone repair, preparation and evaluation of the biological response of human trabecular bone-derived cells and mesenchymal stem cells
author Volkmer, Tiago Moreno
author_facet Volkmer, Tiago Moreno
Magalhães, Joana Cristina Silva
Sousa, Vânia Caldas de
Santos, Luis Alberto dos
Burguera, Elena Fernández
Blanco Garcíaz, Francisco Javier
San Román, Julio
Rodríguez-Lorenzo, Luis M.
author_role author
author2 Magalhães, Joana Cristina Silva
Sousa, Vânia Caldas de
Santos, Luis Alberto dos
Burguera, Elena Fernández
Blanco Garcíaz, Francisco Javier
San Román, Julio
Rodríguez-Lorenzo, Luis M.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Volkmer, Tiago Moreno
Magalhães, Joana Cristina Silva
Sousa, Vânia Caldas de
Santos, Luis Alberto dos
Burguera, Elena Fernández
Blanco Garcíaz, Francisco Javier
San Román, Julio
Rodríguez-Lorenzo, Luis M.
dc.subject.por.fl_str_mv Biomateriais
Engenharia de tecidos
Biopolímeros
topic Biomateriais
Engenharia de tecidos
Biopolímeros
Cryogels
α-tricalcium phosphate
Mesenchymal stem cells
dc.subject.eng.fl_str_mv Cryogels
α-tricalcium phosphate
Mesenchymal stem cells
description The aim of this work is to evaluate the potential of cryogels to be used as scaffolds in tissue engineering. Scaffolds based on the α-tricalcium phosphate reinforced PDMAEMA (Poly(dimethyl aminoethyl methacrylate))/PHEMA (poly(hydroxyethyl methacrylate)) system were prepared and human trabecular bone-derived cells (HTBs) and bone marrow derived-mesenchymal stem cells (BM-MSCs) cultured on them. Several features, such as porosity, pore shape, molecular weight between crosslinks and mesh size, are studied. The most suitable PDMAEMA/PHEMA ratio for cell proliferation has been assessed and the viability, adhesion, proliferation and expression of osteoblastic biochemical markers are evaluated. The PDMAEMA/PHEMA ratio influences the scaffolds porosity. Values between 53% ± 5.7% for a greater content in PHEMA and 75% ± 5.5% for a greater content in PDMAEMA have been obtained. The polymer ratio also modifies the pore shape. A greater content in PDMAEMA leads also to bigger network mesh size. Each of the compositions were non-cytotoxic, the seeded cells remained viable for both BM-MSCs and HTBs. Thus, and based on the structural analysis, specimens with a greater content in PDMAEMA seem to provide a better structural environment for their use as scaffolds for tissue engineering. The α-tricalcium phosphate incorporation into the composition seems to favor the expression of the osteogenic phenotype.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2023-11-25T03:26:19Z
dc.type.driver.fl_str_mv Estrangeiro
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/267620
dc.identifier.issn.pt_BR.fl_str_mv 2073-4360
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dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Polymers [recurso eletrônico]. Basel. Vol. 6 (2014), p. 2510-2525
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