Effect of FSH and insulin on lipogenesis in cultures of Sertoli cells from immature rats
Autor(a) principal: | |
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Data de Publicação: | 1997 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/21122 |
Resumo: | Follicle-stimulating hormone (FSH) and insulin regulate glycide metabolism in Sertoli cells, thus stimulating lactate production. These stimulatory effects of FSH and insulin do not require protein synthesis, suggesting a modulation of enzyme activity and/or regulation of glucose transport. The present investigation was performed to characterize the hormonal control of lipid metabolism in Sertoli cells. The data indicate that FSH and insulin have a regulatory effect on lipid metabolism in Sertoli cells. After 8 h of preincubation with insulin (5 μg/ml), the activity of the enzyme ATP-citrate lyase in cultured Sertoli cells was increased from 0.19 to 0.34 nmol NAD+ formed μg protein-1 min-1. FSH (100 ng/ml) had no effect on this enzyme. Glycerol phosphate dehydrogenase activity was not affected by any of the hormones tested. When Sertoli cells from 19-day old rats were incubated with [1,2-14C]acetate for 90 or 360 min, the [14C] label was present predominantly in triglyceride and phospholipid fractions with minor amounts in other lipids. In Sertoli cells pretreated for 16 h with insulin and FSH, an increase in acetate incorporation into lipids was observed. Most of the label was in esterified lipids and this percentage increased with the time of treatment; this increase was remarkable in triglycerides of control cells (18.8% to 30.6%). Since Sertoli cell triglycerides participate in the control of spermatogenesis, the present data suggest that the hormonal control of lipid metabolism in Sertoli cells is important not only for maintaining the energy of the cell itself, but also for the control of the spermatogenesis process. |
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Guma, Fátima Theresinha Costa RodriguesWajner, MoacirMartini, Lucia HelenaBernard, Elena Aida2010-04-24T04:15:25Z19970100-879Xhttp://hdl.handle.net/10183/21122000226335Follicle-stimulating hormone (FSH) and insulin regulate glycide metabolism in Sertoli cells, thus stimulating lactate production. These stimulatory effects of FSH and insulin do not require protein synthesis, suggesting a modulation of enzyme activity and/or regulation of glucose transport. The present investigation was performed to characterize the hormonal control of lipid metabolism in Sertoli cells. The data indicate that FSH and insulin have a regulatory effect on lipid metabolism in Sertoli cells. After 8 h of preincubation with insulin (5 μg/ml), the activity of the enzyme ATP-citrate lyase in cultured Sertoli cells was increased from 0.19 to 0.34 nmol NAD+ formed μg protein-1 min-1. FSH (100 ng/ml) had no effect on this enzyme. Glycerol phosphate dehydrogenase activity was not affected by any of the hormones tested. When Sertoli cells from 19-day old rats were incubated with [1,2-14C]acetate for 90 or 360 min, the [14C] label was present predominantly in triglyceride and phospholipid fractions with minor amounts in other lipids. In Sertoli cells pretreated for 16 h with insulin and FSH, an increase in acetate incorporation into lipids was observed. Most of the label was in esterified lipids and this percentage increased with the time of treatment; this increase was remarkable in triglycerides of control cells (18.8% to 30.6%). Since Sertoli cell triglycerides participate in the control of spermatogenesis, the present data suggest that the hormonal control of lipid metabolism in Sertoli cells is important not only for maintaining the energy of the cell itself, but also for the control of the spermatogenesis process.application/pdfengBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 30, no. 5, (May 1997), p. 591-597Hormonio foliculo estimulante : Insulina : Lipidios : Celula de sertoliSertoli cellsLipidsFSHInsulinEffect of FSH and insulin on lipogenesis in cultures of Sertoli cells from immature ratsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000226335.pdf000226335.pdfTexto completo (inglês)application/pdf206670http://www.lume.ufrgs.br/bitstream/10183/21122/1/000226335.pdf313578b810f10d9b6f112d5bdb65e225MD51TEXT000226335.pdf.txt000226335.pdf.txtExtracted Texttext/plain27047http://www.lume.ufrgs.br/bitstream/10183/21122/2/000226335.pdf.txtcf351efb54f9f93e5b518f1d9b23a9e7MD52THUMBNAIL000226335.pdf.jpg000226335.pdf.jpgGenerated Thumbnailimage/jpeg1703http://www.lume.ufrgs.br/bitstream/10183/21122/3/000226335.pdf.jpg4b44e2c2536167ce57950a56c786d291MD5310183/211222018-10-08 07:56:28.798oai:www.lume.ufrgs.br:10183/21122Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-08T10:56:28Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Effect of FSH and insulin on lipogenesis in cultures of Sertoli cells from immature rats |
title |
Effect of FSH and insulin on lipogenesis in cultures of Sertoli cells from immature rats |
spellingShingle |
Effect of FSH and insulin on lipogenesis in cultures of Sertoli cells from immature rats Guma, Fátima Theresinha Costa Rodrigues Hormonio foliculo estimulante : Insulina : Lipidios : Celula de sertoli Sertoli cells Lipids FSH Insulin |
title_short |
Effect of FSH and insulin on lipogenesis in cultures of Sertoli cells from immature rats |
title_full |
Effect of FSH and insulin on lipogenesis in cultures of Sertoli cells from immature rats |
title_fullStr |
Effect of FSH and insulin on lipogenesis in cultures of Sertoli cells from immature rats |
title_full_unstemmed |
Effect of FSH and insulin on lipogenesis in cultures of Sertoli cells from immature rats |
title_sort |
Effect of FSH and insulin on lipogenesis in cultures of Sertoli cells from immature rats |
author |
Guma, Fátima Theresinha Costa Rodrigues |
author_facet |
Guma, Fátima Theresinha Costa Rodrigues Wajner, Moacir Martini, Lucia Helena Bernard, Elena Aida |
author_role |
author |
author2 |
Wajner, Moacir Martini, Lucia Helena Bernard, Elena Aida |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Guma, Fátima Theresinha Costa Rodrigues Wajner, Moacir Martini, Lucia Helena Bernard, Elena Aida |
dc.subject.por.fl_str_mv |
Hormonio foliculo estimulante : Insulina : Lipidios : Celula de sertoli |
topic |
Hormonio foliculo estimulante : Insulina : Lipidios : Celula de sertoli Sertoli cells Lipids FSH Insulin |
dc.subject.eng.fl_str_mv |
Sertoli cells Lipids FSH Insulin |
description |
Follicle-stimulating hormone (FSH) and insulin regulate glycide metabolism in Sertoli cells, thus stimulating lactate production. These stimulatory effects of FSH and insulin do not require protein synthesis, suggesting a modulation of enzyme activity and/or regulation of glucose transport. The present investigation was performed to characterize the hormonal control of lipid metabolism in Sertoli cells. The data indicate that FSH and insulin have a regulatory effect on lipid metabolism in Sertoli cells. After 8 h of preincubation with insulin (5 μg/ml), the activity of the enzyme ATP-citrate lyase in cultured Sertoli cells was increased from 0.19 to 0.34 nmol NAD+ formed μg protein-1 min-1. FSH (100 ng/ml) had no effect on this enzyme. Glycerol phosphate dehydrogenase activity was not affected by any of the hormones tested. When Sertoli cells from 19-day old rats were incubated with [1,2-14C]acetate for 90 or 360 min, the [14C] label was present predominantly in triglyceride and phospholipid fractions with minor amounts in other lipids. In Sertoli cells pretreated for 16 h with insulin and FSH, an increase in acetate incorporation into lipids was observed. Most of the label was in esterified lipids and this percentage increased with the time of treatment; this increase was remarkable in triglycerides of control cells (18.8% to 30.6%). Since Sertoli cell triglycerides participate in the control of spermatogenesis, the present data suggest that the hormonal control of lipid metabolism in Sertoli cells is important not only for maintaining the energy of the cell itself, but also for the control of the spermatogenesis process. |
publishDate |
1997 |
dc.date.issued.fl_str_mv |
1997 |
dc.date.accessioned.fl_str_mv |
2010-04-24T04:15:25Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
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publishedVersion |
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http://hdl.handle.net/10183/21122 |
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0100-879X |
dc.identifier.nrb.pt_BR.fl_str_mv |
000226335 |
identifier_str_mv |
0100-879X 000226335 |
url |
http://hdl.handle.net/10183/21122 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 30, no. 5, (May 1997), p. 591-597 |
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openAccess |
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