Protective effect of N-acetyl-L-cysteine and rosuvastatin against oxidative stress in fibroblasts from asymptomatic patients with X-ALD : a preliminary study

• Autor(a) principal: Marchetti, Desirèe Padilha
• Data de Publicação: 2021
• Outros Autores: Ribas, Graziela de Oliveira Schmitt, Donida, Bruna, Brito, Verônica Bidinotto, Moura, Dinara Jaqueline, Coelho, Daniella de Moura, Machado, Andryele Zaffari, Kessler, Rejane Gus, Coitinho, Adriana Simon, Vargas, Carmen Regla
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/225215
• topic: Estresse oxidativo; Acetilcisteína; Adrenoleucodistrofia; Rosuvastatina cálcica; X-linked adrenoleukodystrophy; Fibroblasts; N-acetyl-L-cysteine; Rosuvastatin; Oxidative stress

Introduction: Several studies in the literature have evaluated the role of oxidative stress and adjuvant therapies for X-linked adrenoleukodystrophy (X-ALD). Here, we investigated whether n-acetyl-L-cysteine (NAC) and rosuvastatin (RSV) could influence the generation of reactive species, redox status and nitrative stress in fibroblasts from asymptomatic patients with X-ALD. Methods: Skin biopsy samples were cultured and treated for 2 hours (37 °C) with NAC and RSV. Results: X-ALD fibroblasts generated high levels of reactive oxygen species. These levels were significantly lower in fibroblasts treated with NAC and RSV relative to untreated samples. The X-ALD fibroblasts from asymptomatic patients also had higher catalase activity, and only NAC was able to increase enzyme activity in the samples. Conclusions: Our results indicated that NAC and RSV were able to improve oxidative stress parameters in fibroblasts from asymptomatic patients with X-ALD, showing that adjuvant antioxidant therapy may be a promising treatment strategy for asymptomatic patients with this disease.