Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay

Detalhes bibliográficos
Autor(a) principal: Sierra Arguello, Yuli Melisa
Data de Publicação: 2018
Outros Autores: Furian, Thales Quedi, Perdoncini, Gustavo, Moraes, Hamilton Luiz de Souza, Salle, Carlos Tadeu Pippi, Rodrigues, Laura Beatriz, Santos, Luciana Ruschel dos, Gomes, Marcos José Pereira, Nascimento, Vladimir Pinheiro do
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/182852
Resumo: The objective of this study was to determine fluoroquinolone resistance in Campylobacter spp from poultry and human isolates. Forty-one Campylobacter jejuni isolates (30 of poultry origin and 11 of human origin) and 11 Campylobacter coli isolates (10 of human origin and 1 of poultry origin) were examined for ciprofloxacin, norfloxacin, and nalidixic acid resistance using the minimal inhibitory concentration (MIC) method. Thereafter, the isolates were analyzed by PCR±Restriction Fragment Length Polymorphism (RFLP) assay for detection of Thr-86 mutation. Finally, DNA sequencing was performed for confirmation of gyrA gene mutation. A complete correlation was observed between MICs, PCR-RFLP assay, and sequencing. The results revealed high quinolone resistance rates for C. jejuni (100%) and C. coli (100%) isolates obtained from poultry and moderate resistance for C. jejuni (9.1%) and C. coli (40%) samples of human origin. A mutation in codon 86 of the gyrA gene with a Thr-to-Ile substitution is reported to be the main cause of high resistance to quinolones. This mutation can be analyzed by PCR-RFLP assay, which has been proven to be a simple and fast method for the detection of fluoroquinolone resistance in Campylobacter spp.
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spelling Sierra Arguello, Yuli MelisaFurian, Thales QuediPerdoncini, GustavoMoraes, Hamilton Luiz de SouzaSalle, Carlos Tadeu PippiRodrigues, Laura BeatrizSantos, Luciana Ruschel dosGomes, Marcos José PereiraNascimento, Vladimir Pinheiro do2018-09-27T02:34:37Z20181932-6203http://hdl.handle.net/10183/182852001075036The objective of this study was to determine fluoroquinolone resistance in Campylobacter spp from poultry and human isolates. Forty-one Campylobacter jejuni isolates (30 of poultry origin and 11 of human origin) and 11 Campylobacter coli isolates (10 of human origin and 1 of poultry origin) were examined for ciprofloxacin, norfloxacin, and nalidixic acid resistance using the minimal inhibitory concentration (MIC) method. Thereafter, the isolates were analyzed by PCR±Restriction Fragment Length Polymorphism (RFLP) assay for detection of Thr-86 mutation. Finally, DNA sequencing was performed for confirmation of gyrA gene mutation. A complete correlation was observed between MICs, PCR-RFLP assay, and sequencing. The results revealed high quinolone resistance rates for C. jejuni (100%) and C. coli (100%) isolates obtained from poultry and moderate resistance for C. jejuni (9.1%) and C. coli (40%) samples of human origin. A mutation in codon 86 of the gyrA gene with a Thr-to-Ile substitution is reported to be the main cause of high resistance to quinolones. This mutation can be analyzed by PCR-RFLP assay, which has been proven to be a simple and fast method for the detection of fluoroquinolone resistance in Campylobacter spp.application/pdfengPLOS ONE Journal. San Francisco, CA. Vol. 13, no. 7 (July 2018), e0199974, 9 p.Resistência antimicrobianaFluoroquinolonasCampylobacter jejuniCampylobacter coliAvesSeres humanosPolimorfismo de fragmento de restriçãoAnálise de sequência de DNAFluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assayEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001075036.pdfTexto completo (inglês)application/pdf1925622http://www.lume.ufrgs.br/bitstream/10183/182852/1/001075036.pdf1641a4512cba8cc3cb23b3d2ac036ef9MD51TEXT001075036.pdf.txt001075036.pdf.txtExtracted Texttext/plain30807http://www.lume.ufrgs.br/bitstream/10183/182852/2/001075036.pdf.txt3c3a0c431760b026050bce47fd87dd3fMD52THUMBNAIL001075036.pdf.jpg001075036.pdf.jpgGenerated Thumbnailimage/jpeg2081http://www.lume.ufrgs.br/bitstream/10183/182852/3/001075036.pdf.jpg6ae3c51d55160491729dda62da244c5dMD5310183/1828522018-10-05 08:01:41.353oai:www.lume.ufrgs.br:10183/182852Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-05T11:01:41Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay
title Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay
spellingShingle Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay
Sierra Arguello, Yuli Melisa
Resistência antimicrobiana
Fluoroquinolonas
Campylobacter jejuni
Campylobacter coli
Aves
Seres humanos
Polimorfismo de fragmento de restrição
Análise de sequência de DNA
title_short Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay
title_full Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay
title_fullStr Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay
title_full_unstemmed Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay
title_sort Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay
author Sierra Arguello, Yuli Melisa
author_facet Sierra Arguello, Yuli Melisa
Furian, Thales Quedi
Perdoncini, Gustavo
Moraes, Hamilton Luiz de Souza
Salle, Carlos Tadeu Pippi
Rodrigues, Laura Beatriz
Santos, Luciana Ruschel dos
Gomes, Marcos José Pereira
Nascimento, Vladimir Pinheiro do
author_role author
author2 Furian, Thales Quedi
Perdoncini, Gustavo
Moraes, Hamilton Luiz de Souza
Salle, Carlos Tadeu Pippi
Rodrigues, Laura Beatriz
Santos, Luciana Ruschel dos
Gomes, Marcos José Pereira
Nascimento, Vladimir Pinheiro do
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sierra Arguello, Yuli Melisa
Furian, Thales Quedi
Perdoncini, Gustavo
Moraes, Hamilton Luiz de Souza
Salle, Carlos Tadeu Pippi
Rodrigues, Laura Beatriz
Santos, Luciana Ruschel dos
Gomes, Marcos José Pereira
Nascimento, Vladimir Pinheiro do
dc.subject.por.fl_str_mv Resistência antimicrobiana
Fluoroquinolonas
Campylobacter jejuni
Campylobacter coli
Aves
Seres humanos
Polimorfismo de fragmento de restrição
Análise de sequência de DNA
topic Resistência antimicrobiana
Fluoroquinolonas
Campylobacter jejuni
Campylobacter coli
Aves
Seres humanos
Polimorfismo de fragmento de restrição
Análise de sequência de DNA
description The objective of this study was to determine fluoroquinolone resistance in Campylobacter spp from poultry and human isolates. Forty-one Campylobacter jejuni isolates (30 of poultry origin and 11 of human origin) and 11 Campylobacter coli isolates (10 of human origin and 1 of poultry origin) were examined for ciprofloxacin, norfloxacin, and nalidixic acid resistance using the minimal inhibitory concentration (MIC) method. Thereafter, the isolates were analyzed by PCR±Restriction Fragment Length Polymorphism (RFLP) assay for detection of Thr-86 mutation. Finally, DNA sequencing was performed for confirmation of gyrA gene mutation. A complete correlation was observed between MICs, PCR-RFLP assay, and sequencing. The results revealed high quinolone resistance rates for C. jejuni (100%) and C. coli (100%) isolates obtained from poultry and moderate resistance for C. jejuni (9.1%) and C. coli (40%) samples of human origin. A mutation in codon 86 of the gyrA gene with a Thr-to-Ile substitution is reported to be the main cause of high resistance to quinolones. This mutation can be analyzed by PCR-RFLP assay, which has been proven to be a simple and fast method for the detection of fluoroquinolone resistance in Campylobacter spp.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-09-27T02:34:37Z
dc.date.issued.fl_str_mv 2018
dc.type.driver.fl_str_mv Estrangeiro
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/182852
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.nrb.pt_BR.fl_str_mv 001075036
identifier_str_mv 1932-6203
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url http://hdl.handle.net/10183/182852
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv PLOS ONE Journal. San Francisco, CA. Vol. 13, no. 7 (July 2018), e0199974, 9 p.
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