Prevalence of vitamin D deficiency in women from southern Brazil and association with vitamin D-binding protein levels and GCDBP gene polymorphisms
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/205940 |
Resumo: | Vitamin D deficiency is highly prevalent worldwide, and vitamin D-binding protein (DBP) a major regulator of serum vitamin D levels. The rs4588 and rs7041 polymorphisms of the GC gene constitute the genetic basis of the three major isoforms of circulating DBP (GC1s, GC1f, and GC2), while the rs2282679 variant is located in an important regulatory region of the GC gene. The aim of this study was to assess the prevalence of 25-hydroxyvitamin D [25(OH)D] deficiency and to ascertain whether it is associated with DBP levels and with GC gene variants. Biorepository samples of 443 women aged 20 to 72 years, with no evidence of clinical disease, were analyzed. Circulating levels of 25(OH)D were considered sufficient if ≥20 ng/mL and deficient if <20 ng/mL. Genotype analysis was performed by RT-PCR. Mean age was 53.4±9.4 years; mean BMI was 27.8±5.8 kg/m2. The overall sample had mean 25(OH)D levels of 22.8±8.3 ng/mL; 39.7% of participants had deficient circulating 25 (OH)D levels. Higher prevalence ratios (PR) of 25(OH)D deficiency were found for the CC genotype of rs2282679 (PR 1.74; 95%CI 1.30 to 2.24; p<0.001), GC2 isoform (PR 1.66; 95%CI 1.17 to 2.38; p = 0.005), time since menopause (PR 1.02; 95%CI 1.003 to 1.03, p = 0.016), and HOMA-IR (PR 1.02; 95%CI 1.01 to 1.03, p = 0.004). DBP levels (per 30 μg/mL increase in DBP) were associated with lower PR for 25(OH)D deficiency (PR 0.89; 95%CI 0.80;0.99; p = 0.027). Except for HOMA-IR, these prevalence ratios remained significant after adjustment for age and BMI. In conclusion, the rs2282679 polymorphism and the GC2 isoform of DBP were associated with lower serum DBP levels and with susceptibility to 25 (OH)D deficiency in Brazilian women with no evidence of clinical disease. |
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Santos, Betânia Rodrigues dosCosta, Nathalia Cruz daSilva, Thaís Rasia daOppermann, KarenMagalhães, Jose Antonio de AzevedoCasanova, Gislaine KrolowSpritzer, Poli Mara2020-02-15T04:19:58Z20191932-6203http://hdl.handle.net/10183/205940001111106Vitamin D deficiency is highly prevalent worldwide, and vitamin D-binding protein (DBP) a major regulator of serum vitamin D levels. The rs4588 and rs7041 polymorphisms of the GC gene constitute the genetic basis of the three major isoforms of circulating DBP (GC1s, GC1f, and GC2), while the rs2282679 variant is located in an important regulatory region of the GC gene. The aim of this study was to assess the prevalence of 25-hydroxyvitamin D [25(OH)D] deficiency and to ascertain whether it is associated with DBP levels and with GC gene variants. Biorepository samples of 443 women aged 20 to 72 years, with no evidence of clinical disease, were analyzed. Circulating levels of 25(OH)D were considered sufficient if ≥20 ng/mL and deficient if <20 ng/mL. Genotype analysis was performed by RT-PCR. Mean age was 53.4±9.4 years; mean BMI was 27.8±5.8 kg/m2. The overall sample had mean 25(OH)D levels of 22.8±8.3 ng/mL; 39.7% of participants had deficient circulating 25 (OH)D levels. Higher prevalence ratios (PR) of 25(OH)D deficiency were found for the CC genotype of rs2282679 (PR 1.74; 95%CI 1.30 to 2.24; p<0.001), GC2 isoform (PR 1.66; 95%CI 1.17 to 2.38; p = 0.005), time since menopause (PR 1.02; 95%CI 1.003 to 1.03, p = 0.016), and HOMA-IR (PR 1.02; 95%CI 1.01 to 1.03, p = 0.004). DBP levels (per 30 μg/mL increase in DBP) were associated with lower PR for 25(OH)D deficiency (PR 0.89; 95%CI 0.80;0.99; p = 0.027). Except for HOMA-IR, these prevalence ratios remained significant after adjustment for age and BMI. In conclusion, the rs2282679 polymorphism and the GC2 isoform of DBP were associated with lower serum DBP levels and with susceptibility to 25 (OH)D deficiency in Brazilian women with no evidence of clinical disease.application/pdfengPloS one. San Francisco. Vol. 14, no. 12 (Dez. 2019), e0226215, 11 p.Deficiência de vitamina DProteína de ligação a vitamina DPolimorfismo de nucleotídeo únicoPrevalence of vitamin D deficiency in women from southern Brazil and association with vitamin D-binding protein levels and GCDBP gene polymorphismsEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001111106.pdf.txt001111106.pdf.txtExtracted Texttext/plain40919http://www.lume.ufrgs.br/bitstream/10183/205940/2/001111106.pdf.txtd5aadaa2e34ae1d93078174621d56fa8MD52ORIGINAL001111106.pdfTexto completo (inglês)application/pdf725839http://www.lume.ufrgs.br/bitstream/10183/205940/1/001111106.pdf1770fe2846dd75cc4d03c5f021eeecf5MD5110183/2059402020-02-16 04:16:24.719455oai:www.lume.ufrgs.br:10183/205940Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2020-02-16T07:16:24Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Prevalence of vitamin D deficiency in women from southern Brazil and association with vitamin D-binding protein levels and GCDBP gene polymorphisms |
title |
Prevalence of vitamin D deficiency in women from southern Brazil and association with vitamin D-binding protein levels and GCDBP gene polymorphisms |
spellingShingle |
Prevalence of vitamin D deficiency in women from southern Brazil and association with vitamin D-binding protein levels and GCDBP gene polymorphisms Santos, Betânia Rodrigues dos Deficiência de vitamina D Proteína de ligação a vitamina D Polimorfismo de nucleotídeo único |
title_short |
Prevalence of vitamin D deficiency in women from southern Brazil and association with vitamin D-binding protein levels and GCDBP gene polymorphisms |
title_full |
Prevalence of vitamin D deficiency in women from southern Brazil and association with vitamin D-binding protein levels and GCDBP gene polymorphisms |
title_fullStr |
Prevalence of vitamin D deficiency in women from southern Brazil and association with vitamin D-binding protein levels and GCDBP gene polymorphisms |
title_full_unstemmed |
Prevalence of vitamin D deficiency in women from southern Brazil and association with vitamin D-binding protein levels and GCDBP gene polymorphisms |
title_sort |
Prevalence of vitamin D deficiency in women from southern Brazil and association with vitamin D-binding protein levels and GCDBP gene polymorphisms |
author |
Santos, Betânia Rodrigues dos |
author_facet |
Santos, Betânia Rodrigues dos Costa, Nathalia Cruz da Silva, Thaís Rasia da Oppermann, Karen Magalhães, Jose Antonio de Azevedo Casanova, Gislaine Krolow Spritzer, Poli Mara |
author_role |
author |
author2 |
Costa, Nathalia Cruz da Silva, Thaís Rasia da Oppermann, Karen Magalhães, Jose Antonio de Azevedo Casanova, Gislaine Krolow Spritzer, Poli Mara |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Santos, Betânia Rodrigues dos Costa, Nathalia Cruz da Silva, Thaís Rasia da Oppermann, Karen Magalhães, Jose Antonio de Azevedo Casanova, Gislaine Krolow Spritzer, Poli Mara |
dc.subject.por.fl_str_mv |
Deficiência de vitamina D Proteína de ligação a vitamina D Polimorfismo de nucleotídeo único |
topic |
Deficiência de vitamina D Proteína de ligação a vitamina D Polimorfismo de nucleotídeo único |
description |
Vitamin D deficiency is highly prevalent worldwide, and vitamin D-binding protein (DBP) a major regulator of serum vitamin D levels. The rs4588 and rs7041 polymorphisms of the GC gene constitute the genetic basis of the three major isoforms of circulating DBP (GC1s, GC1f, and GC2), while the rs2282679 variant is located in an important regulatory region of the GC gene. The aim of this study was to assess the prevalence of 25-hydroxyvitamin D [25(OH)D] deficiency and to ascertain whether it is associated with DBP levels and with GC gene variants. Biorepository samples of 443 women aged 20 to 72 years, with no evidence of clinical disease, were analyzed. Circulating levels of 25(OH)D were considered sufficient if ≥20 ng/mL and deficient if <20 ng/mL. Genotype analysis was performed by RT-PCR. Mean age was 53.4±9.4 years; mean BMI was 27.8±5.8 kg/m2. The overall sample had mean 25(OH)D levels of 22.8±8.3 ng/mL; 39.7% of participants had deficient circulating 25 (OH)D levels. Higher prevalence ratios (PR) of 25(OH)D deficiency were found for the CC genotype of rs2282679 (PR 1.74; 95%CI 1.30 to 2.24; p<0.001), GC2 isoform (PR 1.66; 95%CI 1.17 to 2.38; p = 0.005), time since menopause (PR 1.02; 95%CI 1.003 to 1.03, p = 0.016), and HOMA-IR (PR 1.02; 95%CI 1.01 to 1.03, p = 0.004). DBP levels (per 30 μg/mL increase in DBP) were associated with lower PR for 25(OH)D deficiency (PR 0.89; 95%CI 0.80;0.99; p = 0.027). Except for HOMA-IR, these prevalence ratios remained significant after adjustment for age and BMI. In conclusion, the rs2282679 polymorphism and the GC2 isoform of DBP were associated with lower serum DBP levels and with susceptibility to 25 (OH)D deficiency in Brazilian women with no evidence of clinical disease. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019 |
dc.date.accessioned.fl_str_mv |
2020-02-15T04:19:58Z |
dc.type.driver.fl_str_mv |
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1932-6203 |
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001111106 |
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eng |
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PloS one. San Francisco. Vol. 14, no. 12 (Dez. 2019), e0226215, 11 p. |
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