Evaluation of genetic variability in the collared peccary Pecari tajacu and the white-lipped peccary Tayassu pecari by microsatellite markers
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/23417 |
Resumo: | In this study, the microsatellite technique was used to evaluate the genetic variability in populations of collared and white-lipped peccaries kept in captivity. Six primers developed for domestic pigs were used and amplified in both species. They revealed the presence of five polymorphic loci and one monomorphic locus. The polymorphic loci included 4 of the 16 alleles in collared peccaries, and 3 of the 10 alleles in the white-lipped peccaries. Polymorphic information content (PIC) in both species and all the loci was highly informative. The probability of paternity exclusion (PEC), if one of the parents is known, was almost as high in white-lipped peccaries (95.53%) as in the collared (99,48%). The Fst values for collared (0.042) and white-lipped (0.1387) peccaries showed that both populations are not structured. The Fis values for all loci, except ACTG2 in white-lipped peccaries (-0.0275) and in both species (0.1985 to 0.9284 in collared peccaries and 0.3621 to 0.4754 in the white-lipped), revealed a high level of homozygosis, probably caused by inbreeding. Data on heterologous amplification and genetic variability in collared and white-lipped peccaries are presented for the first time. |
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Silva, Roxane WirschumFreitas, Thales Renato Ochotorena deSbalqueiro, Ives Jose2010-06-05T04:17:30Z20101415-4757http://hdl.handle.net/10183/23417000733052In this study, the microsatellite technique was used to evaluate the genetic variability in populations of collared and white-lipped peccaries kept in captivity. Six primers developed for domestic pigs were used and amplified in both species. They revealed the presence of five polymorphic loci and one monomorphic locus. The polymorphic loci included 4 of the 16 alleles in collared peccaries, and 3 of the 10 alleles in the white-lipped peccaries. Polymorphic information content (PIC) in both species and all the loci was highly informative. The probability of paternity exclusion (PEC), if one of the parents is known, was almost as high in white-lipped peccaries (95.53%) as in the collared (99,48%). The Fst values for collared (0.042) and white-lipped (0.1387) peccaries showed that both populations are not structured. The Fis values for all loci, except ACTG2 in white-lipped peccaries (-0.0275) and in both species (0.1985 to 0.9284 in collared peccaries and 0.3621 to 0.4754 in the white-lipped), revealed a high level of homozygosis, probably caused by inbreeding. Data on heterologous amplification and genetic variability in collared and white-lipped peccaries are presented for the first time.application/pdfengGenetics and molecular biology. Ribeirão Preto. Vol. 33, no. 1 (Mar. 2010), p. 62-67MicrossatéliteVariação genéticaTayassu pecariPecari tajacuMicrosatellite markersTayassu pecariPecari tajacuGenetic variabilityAnimals of captivityEvaluation of genetic variability in the collared peccary Pecari tajacu and the white-lipped peccary Tayassu pecari by microsatellite markersinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000733052.pdf.txt000733052.pdf.txtExtracted Texttext/plain25329http://www.lume.ufrgs.br/bitstream/10183/23417/2/000733052.pdf.txt7a4ab309f33ec2de0aa6e10efe6e8247MD52ORIGINAL000733052.pdf000733052.pdfTexto completo (inglês)application/pdf181075http://www.lume.ufrgs.br/bitstream/10183/23417/1/000733052.pdfdd5957984efc703b57a2a8079423d1c9MD51THUMBNAIL000733052.pdf.jpg000733052.pdf.jpgGenerated Thumbnailimage/jpeg1909http://www.lume.ufrgs.br/bitstream/10183/23417/3/000733052.pdf.jpg2eacbe8da7a9810c5f462c54cf008bf5MD5310183/234172024-04-10 06:32:52.096305oai:www.lume.ufrgs.br:10183/23417Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-04-10T09:32:52Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Evaluation of genetic variability in the collared peccary Pecari tajacu and the white-lipped peccary Tayassu pecari by microsatellite markers |
title |
Evaluation of genetic variability in the collared peccary Pecari tajacu and the white-lipped peccary Tayassu pecari by microsatellite markers |
spellingShingle |
Evaluation of genetic variability in the collared peccary Pecari tajacu and the white-lipped peccary Tayassu pecari by microsatellite markers Silva, Roxane Wirschum Microssatélite Variação genética Tayassu pecari Pecari tajacu Microsatellite markers Tayassu pecari Pecari tajacu Genetic variability Animals of captivity |
title_short |
Evaluation of genetic variability in the collared peccary Pecari tajacu and the white-lipped peccary Tayassu pecari by microsatellite markers |
title_full |
Evaluation of genetic variability in the collared peccary Pecari tajacu and the white-lipped peccary Tayassu pecari by microsatellite markers |
title_fullStr |
Evaluation of genetic variability in the collared peccary Pecari tajacu and the white-lipped peccary Tayassu pecari by microsatellite markers |
title_full_unstemmed |
Evaluation of genetic variability in the collared peccary Pecari tajacu and the white-lipped peccary Tayassu pecari by microsatellite markers |
title_sort |
Evaluation of genetic variability in the collared peccary Pecari tajacu and the white-lipped peccary Tayassu pecari by microsatellite markers |
author |
Silva, Roxane Wirschum |
author_facet |
Silva, Roxane Wirschum Freitas, Thales Renato Ochotorena de Sbalqueiro, Ives Jose |
author_role |
author |
author2 |
Freitas, Thales Renato Ochotorena de Sbalqueiro, Ives Jose |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Silva, Roxane Wirschum Freitas, Thales Renato Ochotorena de Sbalqueiro, Ives Jose |
dc.subject.por.fl_str_mv |
Microssatélite Variação genética Tayassu pecari Pecari tajacu |
topic |
Microssatélite Variação genética Tayassu pecari Pecari tajacu Microsatellite markers Tayassu pecari Pecari tajacu Genetic variability Animals of captivity |
dc.subject.eng.fl_str_mv |
Microsatellite markers Tayassu pecari Pecari tajacu Genetic variability Animals of captivity |
description |
In this study, the microsatellite technique was used to evaluate the genetic variability in populations of collared and white-lipped peccaries kept in captivity. Six primers developed for domestic pigs were used and amplified in both species. They revealed the presence of five polymorphic loci and one monomorphic locus. The polymorphic loci included 4 of the 16 alleles in collared peccaries, and 3 of the 10 alleles in the white-lipped peccaries. Polymorphic information content (PIC) in both species and all the loci was highly informative. The probability of paternity exclusion (PEC), if one of the parents is known, was almost as high in white-lipped peccaries (95.53%) as in the collared (99,48%). The Fst values for collared (0.042) and white-lipped (0.1387) peccaries showed that both populations are not structured. The Fis values for all loci, except ACTG2 in white-lipped peccaries (-0.0275) and in both species (0.1985 to 0.9284 in collared peccaries and 0.3621 to 0.4754 in the white-lipped), revealed a high level of homozygosis, probably caused by inbreeding. Data on heterologous amplification and genetic variability in collared and white-lipped peccaries are presented for the first time. |
publishDate |
2010 |
dc.date.accessioned.fl_str_mv |
2010-06-05T04:17:30Z |
dc.date.issued.fl_str_mv |
2010 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
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format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/23417 |
dc.identifier.issn.pt_BR.fl_str_mv |
1415-4757 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000733052 |
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1415-4757 000733052 |
url |
http://hdl.handle.net/10183/23417 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Genetics and molecular biology. Ribeirão Preto. Vol. 33, no. 1 (Mar. 2010), p. 62-67 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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