Intraspecific genetic diversity of Drechslera tritici-repentis as detected by random amplified polymorphic DNA analysis

Detalhes bibliográficos
Autor(a) principal: Santos, Ana Maria Pujol Vieira dos
Data de Publicação: 2002
Outros Autores: Matsumura, Aida Terezinha Santos, Van der Sand, Sueli Terezinha
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/23347
Resumo: The phytopathogenic fungus Drechslera tritici-repentis causes tan spot, an important disease of wheat in the southern Brazilian state of Rio Grande do Sul. Twelve D. tritici-repentis isolates were obtained from wheat seeds from different locations in the state. Their colony morphology on potato dextrose agar and polymorphisms in genomic DNA by the random amplified polymorphic DNA (RAPD) method were investigated. For the RAPD method, 23 primers were tested of which nine were selected for use in the study of D. tritici-repentis polymorphisms. The degree of similarity between isolates was calculated using a simple matching coefficient and dendrograms constructed by the unweighted pair-group method with arithmetical averages (UPGMA). The morphological and RAPD analyses showed intraspecific polymorphisms within the isolates, but it was not possible to establish a relationship between these polymorphisms and the geographical regions from where the host seeds were collected.
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spelling Santos, Ana Maria Pujol Vieira dosMatsumura, Aida Terezinha SantosVan der Sand, Sueli Terezinha2010-06-05T04:17:17Z20021415-4757http://hdl.handle.net/10183/23347000373792The phytopathogenic fungus Drechslera tritici-repentis causes tan spot, an important disease of wheat in the southern Brazilian state of Rio Grande do Sul. Twelve D. tritici-repentis isolates were obtained from wheat seeds from different locations in the state. Their colony morphology on potato dextrose agar and polymorphisms in genomic DNA by the random amplified polymorphic DNA (RAPD) method were investigated. For the RAPD method, 23 primers were tested of which nine were selected for use in the study of D. tritici-repentis polymorphisms. The degree of similarity between isolates was calculated using a simple matching coefficient and dendrograms constructed by the unweighted pair-group method with arithmetical averages (UPGMA). The morphological and RAPD analyses showed intraspecific polymorphisms within the isolates, but it was not possible to establish a relationship between these polymorphisms and the geographical regions from where the host seeds were collected.application/pdfengGenetics and molecular biology. Ribeirão Preto, SP. Vol. 25, no. 2 (June 2002), p. 243-250PolimorfismoDrechslera tritici-repentisDiversidade genéticaDrechslera tritici-repentisRAPDDNA polymorphismGenetic diversityIntraspecific genetic diversity of Drechslera tritici-repentis as detected by random amplified polymorphic DNA analysisinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000373792.pdf000373792.pdfTexto completo (inglês)application/pdf2227652http://www.lume.ufrgs.br/bitstream/10183/23347/1/000373792.pdfae3aa80e1150b40de3d6c60e7fa20305MD51TEXT000373792.pdf.txt000373792.pdf.txtExtracted Texttext/plain25637http://www.lume.ufrgs.br/bitstream/10183/23347/2/000373792.pdf.txt6977c2f38d12a72b2f71bc83566ade46MD52THUMBNAIL000373792.pdf.jpg000373792.pdf.jpgGenerated Thumbnailimage/jpeg1816http://www.lume.ufrgs.br/bitstream/10183/23347/3/000373792.pdf.jpg31251ee828b806396fa46bafea1c8dcdMD5310183/233472023-09-16 03:40:42.417876oai:www.lume.ufrgs.br:10183/23347Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-09-16T06:40:42Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Intraspecific genetic diversity of Drechslera tritici-repentis as detected by random amplified polymorphic DNA analysis
title Intraspecific genetic diversity of Drechslera tritici-repentis as detected by random amplified polymorphic DNA analysis
spellingShingle Intraspecific genetic diversity of Drechslera tritici-repentis as detected by random amplified polymorphic DNA analysis
Santos, Ana Maria Pujol Vieira dos
Polimorfismo
Drechslera tritici-repentis
Diversidade genética
Drechslera tritici-repentis
RAPD
DNA polymorphism
Genetic diversity
title_short Intraspecific genetic diversity of Drechslera tritici-repentis as detected by random amplified polymorphic DNA analysis
title_full Intraspecific genetic diversity of Drechslera tritici-repentis as detected by random amplified polymorphic DNA analysis
title_fullStr Intraspecific genetic diversity of Drechslera tritici-repentis as detected by random amplified polymorphic DNA analysis
title_full_unstemmed Intraspecific genetic diversity of Drechslera tritici-repentis as detected by random amplified polymorphic DNA analysis
title_sort Intraspecific genetic diversity of Drechslera tritici-repentis as detected by random amplified polymorphic DNA analysis
author Santos, Ana Maria Pujol Vieira dos
author_facet Santos, Ana Maria Pujol Vieira dos
Matsumura, Aida Terezinha Santos
Van der Sand, Sueli Terezinha
author_role author
author2 Matsumura, Aida Terezinha Santos
Van der Sand, Sueli Terezinha
author2_role author
author
dc.contributor.author.fl_str_mv Santos, Ana Maria Pujol Vieira dos
Matsumura, Aida Terezinha Santos
Van der Sand, Sueli Terezinha
dc.subject.por.fl_str_mv Polimorfismo
Drechslera tritici-repentis
Diversidade genética
topic Polimorfismo
Drechslera tritici-repentis
Diversidade genética
Drechslera tritici-repentis
RAPD
DNA polymorphism
Genetic diversity
dc.subject.eng.fl_str_mv Drechslera tritici-repentis
RAPD
DNA polymorphism
Genetic diversity
description The phytopathogenic fungus Drechslera tritici-repentis causes tan spot, an important disease of wheat in the southern Brazilian state of Rio Grande do Sul. Twelve D. tritici-repentis isolates were obtained from wheat seeds from different locations in the state. Their colony morphology on potato dextrose agar and polymorphisms in genomic DNA by the random amplified polymorphic DNA (RAPD) method were investigated. For the RAPD method, 23 primers were tested of which nine were selected for use in the study of D. tritici-repentis polymorphisms. The degree of similarity between isolates was calculated using a simple matching coefficient and dendrograms constructed by the unweighted pair-group method with arithmetical averages (UPGMA). The morphological and RAPD analyses showed intraspecific polymorphisms within the isolates, but it was not possible to establish a relationship between these polymorphisms and the geographical regions from where the host seeds were collected.
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2010-06-05T04:17:17Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/23347
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dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Genetics and molecular biology. Ribeirão Preto, SP. Vol. 25, no. 2 (June 2002), p. 243-250
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