Group B Streptococcus detection in pregnant women via culture and PCR methods
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/201273 |
Resumo: | INTRODUCTION: Group B Streptococcus (GBS), a source of neonatal infection, colonizes the gastrointestinal and genitourinary tracts of pregnant women. Routine screening for maternal GBS in late pregnancy and consequent intrapartum antibiotic prophylaxis have reduced the incidence of early-onset GBS neonatal infection. The aim of this study was to evaluate the performance of PCR, compared to culture (gold standard), in GBS colonization screening of pregnant women, and to establish the prevalence of GBS colonization among this population. METHODS: Vaginal introitus and perianal samples were collected from 204 pregnant women, between the 35th and 37th weeks of pregnancy, at the Obstetrics and Gynecology Unit of the University of Caxias do Sul General Hospital between June 2008 and September 2009. All samples were cultured after enrichment in a selective medium and then assayed by culture and PCR methods. RESULTS: The culture and PCR methods yielded detection rates of vaginal/perianal GBS colonization of 22.5% and 26%, respectively (sensitivity 100%; specificity 95.6%; positive and negative predictive values 86.8% and 100%, respectively). A higher prevalence of GBS colonization was detected in the combined vaginal and perianal samples by both culture and PCR assay analyses. CONCLUSIONS: PCR is a faster and more efficient method for GBS screening, allowing for optimal identification of women who should receive intrapartum antibiotic prophylaxis to prevent newborn infection. |
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Wollheim, ClaudiaSperhacke, Rosa DeaFontana, Sabrina Khaler RibeiroVanni, Andréa CristinaKato, Sergio KakutaAraújo, Patrícia Regina deBarth, Afonso LuisMadi, José Mauro2019-11-02T03:51:51Z20170037-8682http://hdl.handle.net/10183/201273001104004INTRODUCTION: Group B Streptococcus (GBS), a source of neonatal infection, colonizes the gastrointestinal and genitourinary tracts of pregnant women. Routine screening for maternal GBS in late pregnancy and consequent intrapartum antibiotic prophylaxis have reduced the incidence of early-onset GBS neonatal infection. The aim of this study was to evaluate the performance of PCR, compared to culture (gold standard), in GBS colonization screening of pregnant women, and to establish the prevalence of GBS colonization among this population. METHODS: Vaginal introitus and perianal samples were collected from 204 pregnant women, between the 35th and 37th weeks of pregnancy, at the Obstetrics and Gynecology Unit of the University of Caxias do Sul General Hospital between June 2008 and September 2009. All samples were cultured after enrichment in a selective medium and then assayed by culture and PCR methods. RESULTS: The culture and PCR methods yielded detection rates of vaginal/perianal GBS colonization of 22.5% and 26%, respectively (sensitivity 100%; specificity 95.6%; positive and negative predictive values 86.8% and 100%, respectively). A higher prevalence of GBS colonization was detected in the combined vaginal and perianal samples by both culture and PCR assay analyses. CONCLUSIONS: PCR is a faster and more efficient method for GBS screening, allowing for optimal identification of women who should receive intrapartum antibiotic prophylaxis to prevent newborn infection.application/pdfengRevista da Sociedade Brasileira de Medicina Tropical. Vol. 50, n. 2 (mar./abr. 2017), p. 179-183StreptococcusGestantesGroup B StreptococcusPregnant womenCulturePCRGroup B Streptococcus detection in pregnant women via culture and PCR methodsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001104004.pdf.txt001104004.pdf.txtExtracted Texttext/plain22801http://www.lume.ufrgs.br/bitstream/10183/201273/2/001104004.pdf.txtc91261404c565444beffeb550bae6d7dMD52ORIGINAL001104004.pdfTexto completo (inglês)application/pdf855186http://www.lume.ufrgs.br/bitstream/10183/201273/1/001104004.pdfe76cfd19ea369ae8e9b6495924d48d87MD5110183/2012732019-11-03 03:52:02.697326oai:www.lume.ufrgs.br:10183/201273Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-11-03T05:52:02Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Group B Streptococcus detection in pregnant women via culture and PCR methods |
title |
Group B Streptococcus detection in pregnant women via culture and PCR methods |
spellingShingle |
Group B Streptococcus detection in pregnant women via culture and PCR methods Wollheim, Claudia Streptococcus Gestantes Group B Streptococcus Pregnant women Culture PCR |
title_short |
Group B Streptococcus detection in pregnant women via culture and PCR methods |
title_full |
Group B Streptococcus detection in pregnant women via culture and PCR methods |
title_fullStr |
Group B Streptococcus detection in pregnant women via culture and PCR methods |
title_full_unstemmed |
Group B Streptococcus detection in pregnant women via culture and PCR methods |
title_sort |
Group B Streptococcus detection in pregnant women via culture and PCR methods |
author |
Wollheim, Claudia |
author_facet |
Wollheim, Claudia Sperhacke, Rosa Dea Fontana, Sabrina Khaler Ribeiro Vanni, Andréa Cristina Kato, Sergio Kakuta Araújo, Patrícia Regina de Barth, Afonso Luis Madi, José Mauro |
author_role |
author |
author2 |
Sperhacke, Rosa Dea Fontana, Sabrina Khaler Ribeiro Vanni, Andréa Cristina Kato, Sergio Kakuta Araújo, Patrícia Regina de Barth, Afonso Luis Madi, José Mauro |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Wollheim, Claudia Sperhacke, Rosa Dea Fontana, Sabrina Khaler Ribeiro Vanni, Andréa Cristina Kato, Sergio Kakuta Araújo, Patrícia Regina de Barth, Afonso Luis Madi, José Mauro |
dc.subject.por.fl_str_mv |
Streptococcus Gestantes |
topic |
Streptococcus Gestantes Group B Streptococcus Pregnant women Culture PCR |
dc.subject.eng.fl_str_mv |
Group B Streptococcus Pregnant women Culture PCR |
description |
INTRODUCTION: Group B Streptococcus (GBS), a source of neonatal infection, colonizes the gastrointestinal and genitourinary tracts of pregnant women. Routine screening for maternal GBS in late pregnancy and consequent intrapartum antibiotic prophylaxis have reduced the incidence of early-onset GBS neonatal infection. The aim of this study was to evaluate the performance of PCR, compared to culture (gold standard), in GBS colonization screening of pregnant women, and to establish the prevalence of GBS colonization among this population. METHODS: Vaginal introitus and perianal samples were collected from 204 pregnant women, between the 35th and 37th weeks of pregnancy, at the Obstetrics and Gynecology Unit of the University of Caxias do Sul General Hospital between June 2008 and September 2009. All samples were cultured after enrichment in a selective medium and then assayed by culture and PCR methods. RESULTS: The culture and PCR methods yielded detection rates of vaginal/perianal GBS colonization of 22.5% and 26%, respectively (sensitivity 100%; specificity 95.6%; positive and negative predictive values 86.8% and 100%, respectively). A higher prevalence of GBS colonization was detected in the combined vaginal and perianal samples by both culture and PCR assay analyses. CONCLUSIONS: PCR is a faster and more efficient method for GBS screening, allowing for optimal identification of women who should receive intrapartum antibiotic prophylaxis to prevent newborn infection. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2019-11-02T03:51:51Z |
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info:eu-repo/semantics/article info:eu-repo/semantics/other |
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info:eu-repo/semantics/publishedVersion |
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publishedVersion |
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http://hdl.handle.net/10183/201273 |
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0037-8682 |
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001104004 |
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http://hdl.handle.net/10183/201273 |
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eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical. Vol. 50, n. 2 (mar./abr. 2017), p. 179-183 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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