Efficacy of carotenoid-loaded gelatin nanoparticles in reducing plasma cytokines and adipocyte hypertrophy in wistar rats

• Autor(a) principal: Queiroz, Jaluza Luana Carvalho de
• Data de Publicação: 2023
• Outros Autores: Medeiros, Isaiane, Lima , Mayara Santa Rosa, Carvalho, Fabiana Maria Coimbra de, Camillo, Christina da Silva, Santos, Pedro Paulo de Andrade, Guerra, Gerlane Coelho Bernardo, Silva, Valéria C. da, Schroeder, Helena Trevisan, Krause, Maurício da Silva, Morais, Ana Heloneida de Araujo, Passos, Thaís Souza
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/280012
• topic: Nanopartículas; Adiposidade; Anti-inflamatórios; Carotenóides; Cucumis melo L.; Nanoparticles; Anti-inflammatory agents; Adiposity

The present study investigated the effect of gelatin-based nanoparticles (EPG) loaded with a carotenoid-rich crude extract (CE) on systemic and adipose tissue inflammatory response in a model with inflammation induced by a high glycemic index and high glycemic load diet (HGLI). Nanopar- ticles synthesized were characterized by different physical and chemical methods. The in vivo investigation evaluated Wistar rats (n = 20, 11 days, adult male with 21 weeks) subdivided into un- treated (HGLI diet), conventional treatment (nutritionally adequate diet), treatment 1 (HGLI + crude extract (12.5 mg/kg)), and treatment 2 (HGLI + EPG (50 mg/kg)) groups. Dietary intake, caloric intake and efficiency, weight, inflammatory cytokines tissue concentration, visceral adipose tissue (VAT) weight, histopathological analysis, and antioxidant activity in plasma and VAT were inves- tigated. EPG showed the same physical and chemical characteristics as previous batches (95.2 nm, smooth surface, and chemical interactions between materials). The EPG-treated group was the only group promoting negative ∆dietary intake, ∆caloric efficiency, and ∆weight. In addition, it presented a significant reduction (p < 0.05) in IL-6 and leptin levels and a greater presence of multilocular adipocytes. The results suggest that EPG can act as a nutraceutical in adjuvant therapy for treating inflammatory diseases associated with adipose tissue accumulation.