Safety and effectiveness of copaiba oleoresin (c. reticulata ducke) on inflammation and tissue repair of oral wounds in rats

• Autor(a) principal: Alvarenga, María Olimpia Paz
• Data de Publicação: 2020
• Outros Autores: Bittencourt, Leonardo de Oliveira, Mendes, Paulo Fernando Santos, Ribeiro, Julia Turra, Lameira, Osmar Alves, Monteiro, Marta Chagas, Barboza, Carlos Augusto Galvão, Martins, Manoela Domingues, Lima, Rafael Rodrigues
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/226046
• topic: Cicatrização; Terapias complementares; Anti-inflamatórios; Copaiba oleoresin; Wound healing; Alternative therapy; Natural anti-inflammatory; C. reticulata Ducke

In traditional communities of the Brazilian Amazon, the copaiba oleoresin (C. reticulata Ducke) is widely known for its therapeutic activity, especially its wound healing and anti-inflammatory actions. Our study aimed to evaluate these effects in oral lesions and the safety of the dosage proposed. A punch biopsy wound was induced on the ventral surface of the tongue of forty-five male Wistar rats under anesthesia. Animals were randomly allocated to one of three groups based on the treatment: control, corticoid and copaiba. A daily dose of each treatment and vehicle was administrated by oral gavage for three consecutive days. Sample collections took place on the third, seventh and 15th days post-wounding for clinical and histopathological analyses. Blood was collected on the third and seventh days for kidneys and liver function tests. Semi-quantitative analyses were performed based on scores of inflammation and reepithelization. Tissue collagen deposition was detected by PicroSirius red staining. Copaiba-treated wounds revealed a smaller wound area, decreased of acute inflammatory reaction and enhanced reepithelization. The levels of kidney and liver function tests did not reveal presence of damage post-treatments. Our findings suggest that copaiba oleoresin is a safe and effective alternative therapy for inflammation and tissue repair of oral wounds in this animal model