Biological activity of bacteria isolated from wetland sediments collected from a conservation unit in the southern region of Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/262344 |
Resumo: | Wetlands are ecosystems rich in biodiversity and their ecological importance is recognized worldwide. Sediment samples were subjected to physical-chemical analysis and organic carbon content varied from 3.0% to 4.8%, the clay between 32 and 40%, silt with 41% and 43%, sand coarse varied between 6 and 11% and fi ne sand between 7 and 16%. The nitrogen values varied from 0.25% to 0.48%, the pH from 5.4 to 7.5 and the humidity varied from 44 to 56%. The selected isolates were evaluated for enzymatic properties and 64% showed positive results for amylase, 16% for gelatinase, 37% for lipase, 91% for protease and 2.7% for inulinase. Six bacterial isolates were selected for the overlapping assay and Bacillus sp. sed 2.2 showed inhibitory activity against Corynebacterium fi mi NCTC 7547, and the antimicrobial substance was partially purifi ed. The characterization of the substance was carried and the substance was stable at 100° C for up to 10 minutes and sensitive to the enzymes papain and trypsin. This substance was active against some species of Listeria, including Listeria monocytogenes ATCC 7644. The microorganims obtained from sediment samples were important sources of bioactive compounds, including enzymes and peptides, being a source of bioactive compounds to be studied. |
id |
UFRGS-2_5163a3836f1541108021892a798f3912 |
---|---|
oai_identifier_str |
oai:www.lume.ufrgs.br:10183/262344 |
network_acronym_str |
UFRGS-2 |
network_name_str |
Repositório Institucional da UFRGS |
repository_id_str |
|
spelling |
Jankoski, Priscila RibeiroCorrea, Ana Paula FolmerBrandelli, AdrianoMotta, Amanda de Souza da2023-07-19T03:39:02Z20210001-3765http://hdl.handle.net/10183/262344001168302Wetlands are ecosystems rich in biodiversity and their ecological importance is recognized worldwide. Sediment samples were subjected to physical-chemical analysis and organic carbon content varied from 3.0% to 4.8%, the clay between 32 and 40%, silt with 41% and 43%, sand coarse varied between 6 and 11% and fi ne sand between 7 and 16%. The nitrogen values varied from 0.25% to 0.48%, the pH from 5.4 to 7.5 and the humidity varied from 44 to 56%. The selected isolates were evaluated for enzymatic properties and 64% showed positive results for amylase, 16% for gelatinase, 37% for lipase, 91% for protease and 2.7% for inulinase. Six bacterial isolates were selected for the overlapping assay and Bacillus sp. sed 2.2 showed inhibitory activity against Corynebacterium fi mi NCTC 7547, and the antimicrobial substance was partially purifi ed. The characterization of the substance was carried and the substance was stable at 100° C for up to 10 minutes and sensitive to the enzymes papain and trypsin. This substance was active against some species of Listeria, including Listeria monocytogenes ATCC 7644. The microorganims obtained from sediment samples were important sources of bioactive compounds, including enzymes and peptides, being a source of bioactive compounds to be studied.application/pdfengAnais da Academia Brasileira de Ciências. Rio de Janeiro. Vol. 93, n. 3 (2021), e20191269, 15 p.Atividade antimicrobianaBacillusCompostos bioativosPeptideoAntimicrobialBacillusBioactive propertiesPeptide purificationWetlandsBiological activity of bacteria isolated from wetland sediments collected from a conservation unit in the southern region of Brazilinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001168302.pdf.txt001168302.pdf.txtExtracted Texttext/plain50533http://www.lume.ufrgs.br/bitstream/10183/262344/2/001168302.pdf.txt2ee1663b45dc804129b0d82a5e585aa9MD52ORIGINAL001168302.pdfTexto completo (inglês)application/pdf465892http://www.lume.ufrgs.br/bitstream/10183/262344/1/001168302.pdf08cfd500abc98622f7c02b35825c7f03MD5110183/2623442023-07-20 03:35:17.01238oai:www.lume.ufrgs.br:10183/262344Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-07-20T06:35:17Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Biological activity of bacteria isolated from wetland sediments collected from a conservation unit in the southern region of Brazil |
title |
Biological activity of bacteria isolated from wetland sediments collected from a conservation unit in the southern region of Brazil |
spellingShingle |
Biological activity of bacteria isolated from wetland sediments collected from a conservation unit in the southern region of Brazil Jankoski, Priscila Ribeiro Atividade antimicrobiana Bacillus Compostos bioativos Peptideo Antimicrobial Bacillus Bioactive properties Peptide purification Wetlands |
title_short |
Biological activity of bacteria isolated from wetland sediments collected from a conservation unit in the southern region of Brazil |
title_full |
Biological activity of bacteria isolated from wetland sediments collected from a conservation unit in the southern region of Brazil |
title_fullStr |
Biological activity of bacteria isolated from wetland sediments collected from a conservation unit in the southern region of Brazil |
title_full_unstemmed |
Biological activity of bacteria isolated from wetland sediments collected from a conservation unit in the southern region of Brazil |
title_sort |
Biological activity of bacteria isolated from wetland sediments collected from a conservation unit in the southern region of Brazil |
author |
Jankoski, Priscila Ribeiro |
author_facet |
Jankoski, Priscila Ribeiro Correa, Ana Paula Folmer Brandelli, Adriano Motta, Amanda de Souza da |
author_role |
author |
author2 |
Correa, Ana Paula Folmer Brandelli, Adriano Motta, Amanda de Souza da |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Jankoski, Priscila Ribeiro Correa, Ana Paula Folmer Brandelli, Adriano Motta, Amanda de Souza da |
dc.subject.por.fl_str_mv |
Atividade antimicrobiana Bacillus Compostos bioativos Peptideo |
topic |
Atividade antimicrobiana Bacillus Compostos bioativos Peptideo Antimicrobial Bacillus Bioactive properties Peptide purification Wetlands |
dc.subject.eng.fl_str_mv |
Antimicrobial Bacillus Bioactive properties Peptide purification Wetlands |
description |
Wetlands are ecosystems rich in biodiversity and their ecological importance is recognized worldwide. Sediment samples were subjected to physical-chemical analysis and organic carbon content varied from 3.0% to 4.8%, the clay between 32 and 40%, silt with 41% and 43%, sand coarse varied between 6 and 11% and fi ne sand between 7 and 16%. The nitrogen values varied from 0.25% to 0.48%, the pH from 5.4 to 7.5 and the humidity varied from 44 to 56%. The selected isolates were evaluated for enzymatic properties and 64% showed positive results for amylase, 16% for gelatinase, 37% for lipase, 91% for protease and 2.7% for inulinase. Six bacterial isolates were selected for the overlapping assay and Bacillus sp. sed 2.2 showed inhibitory activity against Corynebacterium fi mi NCTC 7547, and the antimicrobial substance was partially purifi ed. The characterization of the substance was carried and the substance was stable at 100° C for up to 10 minutes and sensitive to the enzymes papain and trypsin. This substance was active against some species of Listeria, including Listeria monocytogenes ATCC 7644. The microorganims obtained from sediment samples were important sources of bioactive compounds, including enzymes and peptides, being a source of bioactive compounds to be studied. |
publishDate |
2021 |
dc.date.issued.fl_str_mv |
2021 |
dc.date.accessioned.fl_str_mv |
2023-07-19T03:39:02Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/262344 |
dc.identifier.issn.pt_BR.fl_str_mv |
0001-3765 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001168302 |
identifier_str_mv |
0001-3765 001168302 |
url |
http://hdl.handle.net/10183/262344 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Anais da Academia Brasileira de Ciências. Rio de Janeiro. Vol. 93, n. 3 (2021), e20191269, 15 p. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Repositório Institucional da UFRGS |
collection |
Repositório Institucional da UFRGS |
bitstream.url.fl_str_mv |
http://www.lume.ufrgs.br/bitstream/10183/262344/2/001168302.pdf.txt http://www.lume.ufrgs.br/bitstream/10183/262344/1/001168302.pdf |
bitstream.checksum.fl_str_mv |
2ee1663b45dc804129b0d82a5e585aa9 08cfd500abc98622f7c02b35825c7f03 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
|
_version_ |
1801225092175233024 |