Investigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticides
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/150084 |
Resumo: | Agricultural workers involved in the harvest of tobacco crops are regularly exposed to large quantities of pesticides. In order to determine how this exposure to pesticides induces genetic alterations in these workers, blood samples were obtained from 77 exposed individuals, as well as from 60 unexposed subjects. DNA damage was analyzed by the Comet assay and by the micronucleus (MN) test. The antioxidant profi le was evaluated by activity of superoxide dismutase (SOD), and the polymorphism of gene PON1 was used as a susceptibility biomarker. The content of inorganic elements in the blood samples was determined by PIXE analysis. Our results demonstrated that the damage frequency, damage index, the MN frequency, and the SOD activity were signifi cantly elevated in the exposed relative to the unexposed group. A modulation of the MN results for the PON1 gene was observed in the exposed group. The concentrations of inorganic elements in the exposed group were higher compared to those of the unexposed group. In this study, we observed that genetic damage, and change in oxidative balance were induced by the exposure of workers to complex mixtures of pesticides in the presence of inorganic compounds, whereby an infl uence of the genotype was evident. |
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Alves, Jodel da SilvaSilva, Fernanda Rabaioli daSilva, Gabrieli Flesch daSalvador, MirianKvitko, KatiaRohr, PaulaSantos, Carla Eliete Iochims dosDias, Johnny FerrazHenriques, João Antonio PêgasSilva, Juliana da2016-12-15T02:15:26Z20160001-3765http://hdl.handle.net/10183/150084001001452Agricultural workers involved in the harvest of tobacco crops are regularly exposed to large quantities of pesticides. In order to determine how this exposure to pesticides induces genetic alterations in these workers, blood samples were obtained from 77 exposed individuals, as well as from 60 unexposed subjects. DNA damage was analyzed by the Comet assay and by the micronucleus (MN) test. The antioxidant profi le was evaluated by activity of superoxide dismutase (SOD), and the polymorphism of gene PON1 was used as a susceptibility biomarker. The content of inorganic elements in the blood samples was determined by PIXE analysis. Our results demonstrated that the damage frequency, damage index, the MN frequency, and the SOD activity were signifi cantly elevated in the exposed relative to the unexposed group. A modulation of the MN results for the PON1 gene was observed in the exposed group. The concentrations of inorganic elements in the exposed group were higher compared to those of the unexposed group. In this study, we observed that genetic damage, and change in oxidative balance were induced by the exposure of workers to complex mixtures of pesticides in the presence of inorganic compounds, whereby an infl uence of the genotype was evident.application/pdfengAnais da Academia Brasileira de Ciências. Rio de Janeiro. Vol. 88, n. 1 (mar. 2016), p. 349-360PesticidasExposição ocupacionalEnsaio cometaTestes para micronúcleosDano ao DNASuperóxido dismutasePesticidesComet assayMicronucleus testSuperoxide dismutase activityPON1 geneInvestigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticidesinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001001452.pdf001001452.pdfTexto completo (inglês)application/pdf814323http://www.lume.ufrgs.br/bitstream/10183/150084/1/001001452.pdfccd8a3904b92fc1b4f77c4168ab09565MD51TEXT001001452.pdf.txt001001452.pdf.txtExtracted Texttext/plain42412http://www.lume.ufrgs.br/bitstream/10183/150084/2/001001452.pdf.txt419ceccd47d09e58deccebf9111457deMD52THUMBNAIL001001452.pdf.jpg001001452.pdf.jpgGenerated Thumbnailimage/jpeg1894http://www.lume.ufrgs.br/bitstream/10183/150084/3/001001452.pdf.jpgf5f2a06af34f26e5b3094ac80f8e4654MD5310183/1500842024-04-28 06:55:44.10577oai:www.lume.ufrgs.br:10183/150084Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-04-28T09:55:44Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Investigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticides |
title |
Investigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticides |
spellingShingle |
Investigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticides Alves, Jodel da Silva Pesticidas Exposição ocupacional Ensaio cometa Testes para micronúcleos Dano ao DNA Superóxido dismutase Pesticides Comet assay Micronucleus test Superoxide dismutase activity PON1 gene |
title_short |
Investigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticides |
title_full |
Investigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticides |
title_fullStr |
Investigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticides |
title_full_unstemmed |
Investigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticides |
title_sort |
Investigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticides |
author |
Alves, Jodel da Silva |
author_facet |
Alves, Jodel da Silva Silva, Fernanda Rabaioli da Silva, Gabrieli Flesch da Salvador, Mirian Kvitko, Katia Rohr, Paula Santos, Carla Eliete Iochims dos Dias, Johnny Ferraz Henriques, João Antonio Pêgas Silva, Juliana da |
author_role |
author |
author2 |
Silva, Fernanda Rabaioli da Silva, Gabrieli Flesch da Salvador, Mirian Kvitko, Katia Rohr, Paula Santos, Carla Eliete Iochims dos Dias, Johnny Ferraz Henriques, João Antonio Pêgas Silva, Juliana da |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Alves, Jodel da Silva Silva, Fernanda Rabaioli da Silva, Gabrieli Flesch da Salvador, Mirian Kvitko, Katia Rohr, Paula Santos, Carla Eliete Iochims dos Dias, Johnny Ferraz Henriques, João Antonio Pêgas Silva, Juliana da |
dc.subject.por.fl_str_mv |
Pesticidas Exposição ocupacional Ensaio cometa Testes para micronúcleos Dano ao DNA Superóxido dismutase |
topic |
Pesticidas Exposição ocupacional Ensaio cometa Testes para micronúcleos Dano ao DNA Superóxido dismutase Pesticides Comet assay Micronucleus test Superoxide dismutase activity PON1 gene |
dc.subject.eng.fl_str_mv |
Pesticides Comet assay Micronucleus test Superoxide dismutase activity PON1 gene |
description |
Agricultural workers involved in the harvest of tobacco crops are regularly exposed to large quantities of pesticides. In order to determine how this exposure to pesticides induces genetic alterations in these workers, blood samples were obtained from 77 exposed individuals, as well as from 60 unexposed subjects. DNA damage was analyzed by the Comet assay and by the micronucleus (MN) test. The antioxidant profi le was evaluated by activity of superoxide dismutase (SOD), and the polymorphism of gene PON1 was used as a susceptibility biomarker. The content of inorganic elements in the blood samples was determined by PIXE analysis. Our results demonstrated that the damage frequency, damage index, the MN frequency, and the SOD activity were signifi cantly elevated in the exposed relative to the unexposed group. A modulation of the MN results for the PON1 gene was observed in the exposed group. The concentrations of inorganic elements in the exposed group were higher compared to those of the unexposed group. In this study, we observed that genetic damage, and change in oxidative balance were induced by the exposure of workers to complex mixtures of pesticides in the presence of inorganic compounds, whereby an infl uence of the genotype was evident. |
publishDate |
2016 |
dc.date.accessioned.fl_str_mv |
2016-12-15T02:15:26Z |
dc.date.issued.fl_str_mv |
2016 |
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info:eu-repo/semantics/article info:eu-repo/semantics/other |
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publishedVersion |
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http://hdl.handle.net/10183/150084 |
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0001-3765 |
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001001452 |
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http://hdl.handle.net/10183/150084 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Anais da Academia Brasileira de Ciências. Rio de Janeiro. Vol. 88, n. 1 (mar. 2016), p. 349-360 |
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openAccess |
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