Investigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticides

Detalhes bibliográficos
Autor(a) principal: Alves, Jodel da Silva
Data de Publicação: 2016
Outros Autores: Silva, Fernanda Rabaioli da, Silva, Gabrieli Flesch da, Salvador, Mirian, Kvitko, Katia, Rohr, Paula, Santos, Carla Eliete Iochims dos, Dias, Johnny Ferraz, Henriques, João Antonio Pêgas, Silva, Juliana da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/150084
Resumo: Agricultural workers involved in the harvest of tobacco crops are regularly exposed to large quantities of pesticides. In order to determine how this exposure to pesticides induces genetic alterations in these workers, blood samples were obtained from 77 exposed individuals, as well as from 60 unexposed subjects. DNA damage was analyzed by the Comet assay and by the micronucleus (MN) test. The antioxidant profi le was evaluated by activity of superoxide dismutase (SOD), and the polymorphism of gene PON1 was used as a susceptibility biomarker. The content of inorganic elements in the blood samples was determined by PIXE analysis. Our results demonstrated that the damage frequency, damage index, the MN frequency, and the SOD activity were signifi cantly elevated in the exposed relative to the unexposed group. A modulation of the MN results for the PON1 gene was observed in the exposed group. The concentrations of inorganic elements in the exposed group were higher compared to those of the unexposed group. In this study, we observed that genetic damage, and change in oxidative balance were induced by the exposure of workers to complex mixtures of pesticides in the presence of inorganic compounds, whereby an infl uence of the genotype was evident.
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spelling Alves, Jodel da SilvaSilva, Fernanda Rabaioli daSilva, Gabrieli Flesch daSalvador, MirianKvitko, KatiaRohr, PaulaSantos, Carla Eliete Iochims dosDias, Johnny FerrazHenriques, João Antonio PêgasSilva, Juliana da2016-12-15T02:15:26Z20160001-3765http://hdl.handle.net/10183/150084001001452Agricultural workers involved in the harvest of tobacco crops are regularly exposed to large quantities of pesticides. In order to determine how this exposure to pesticides induces genetic alterations in these workers, blood samples were obtained from 77 exposed individuals, as well as from 60 unexposed subjects. DNA damage was analyzed by the Comet assay and by the micronucleus (MN) test. The antioxidant profi le was evaluated by activity of superoxide dismutase (SOD), and the polymorphism of gene PON1 was used as a susceptibility biomarker. The content of inorganic elements in the blood samples was determined by PIXE analysis. Our results demonstrated that the damage frequency, damage index, the MN frequency, and the SOD activity were signifi cantly elevated in the exposed relative to the unexposed group. A modulation of the MN results for the PON1 gene was observed in the exposed group. The concentrations of inorganic elements in the exposed group were higher compared to those of the unexposed group. In this study, we observed that genetic damage, and change in oxidative balance were induced by the exposure of workers to complex mixtures of pesticides in the presence of inorganic compounds, whereby an infl uence of the genotype was evident.application/pdfengAnais da Academia Brasileira de Ciências. Rio de Janeiro. Vol. 88, n. 1 (mar. 2016), p. 349-360PesticidasExposição ocupacionalEnsaio cometaTestes para micronúcleosDano ao DNASuperóxido dismutasePesticidesComet assayMicronucleus testSuperoxide dismutase activityPON1 geneInvestigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticidesinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001001452.pdf001001452.pdfTexto completo (inglês)application/pdf814323http://www.lume.ufrgs.br/bitstream/10183/150084/1/001001452.pdfccd8a3904b92fc1b4f77c4168ab09565MD51TEXT001001452.pdf.txt001001452.pdf.txtExtracted Texttext/plain42412http://www.lume.ufrgs.br/bitstream/10183/150084/2/001001452.pdf.txt419ceccd47d09e58deccebf9111457deMD52THUMBNAIL001001452.pdf.jpg001001452.pdf.jpgGenerated Thumbnailimage/jpeg1894http://www.lume.ufrgs.br/bitstream/10183/150084/3/001001452.pdf.jpgf5f2a06af34f26e5b3094ac80f8e4654MD5310183/1500842024-04-28 06:55:44.10577oai:www.lume.ufrgs.br:10183/150084Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-04-28T09:55:44Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Investigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticides
title Investigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticides
spellingShingle Investigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticides
Alves, Jodel da Silva
Pesticidas
Exposição ocupacional
Ensaio cometa
Testes para micronúcleos
Dano ao DNA
Superóxido dismutase
Pesticides
Comet assay
Micronucleus test
Superoxide dismutase activity
PON1 gene
title_short Investigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticides
title_full Investigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticides
title_fullStr Investigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticides
title_full_unstemmed Investigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticides
title_sort Investigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticides
author Alves, Jodel da Silva
author_facet Alves, Jodel da Silva
Silva, Fernanda Rabaioli da
Silva, Gabrieli Flesch da
Salvador, Mirian
Kvitko, Katia
Rohr, Paula
Santos, Carla Eliete Iochims dos
Dias, Johnny Ferraz
Henriques, João Antonio Pêgas
Silva, Juliana da
author_role author
author2 Silva, Fernanda Rabaioli da
Silva, Gabrieli Flesch da
Salvador, Mirian
Kvitko, Katia
Rohr, Paula
Santos, Carla Eliete Iochims dos
Dias, Johnny Ferraz
Henriques, João Antonio Pêgas
Silva, Juliana da
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Alves, Jodel da Silva
Silva, Fernanda Rabaioli da
Silva, Gabrieli Flesch da
Salvador, Mirian
Kvitko, Katia
Rohr, Paula
Santos, Carla Eliete Iochims dos
Dias, Johnny Ferraz
Henriques, João Antonio Pêgas
Silva, Juliana da
dc.subject.por.fl_str_mv Pesticidas
Exposição ocupacional
Ensaio cometa
Testes para micronúcleos
Dano ao DNA
Superóxido dismutase
topic Pesticidas
Exposição ocupacional
Ensaio cometa
Testes para micronúcleos
Dano ao DNA
Superóxido dismutase
Pesticides
Comet assay
Micronucleus test
Superoxide dismutase activity
PON1 gene
dc.subject.eng.fl_str_mv Pesticides
Comet assay
Micronucleus test
Superoxide dismutase activity
PON1 gene
description Agricultural workers involved in the harvest of tobacco crops are regularly exposed to large quantities of pesticides. In order to determine how this exposure to pesticides induces genetic alterations in these workers, blood samples were obtained from 77 exposed individuals, as well as from 60 unexposed subjects. DNA damage was analyzed by the Comet assay and by the micronucleus (MN) test. The antioxidant profi le was evaluated by activity of superoxide dismutase (SOD), and the polymorphism of gene PON1 was used as a susceptibility biomarker. The content of inorganic elements in the blood samples was determined by PIXE analysis. Our results demonstrated that the damage frequency, damage index, the MN frequency, and the SOD activity were signifi cantly elevated in the exposed relative to the unexposed group. A modulation of the MN results for the PON1 gene was observed in the exposed group. The concentrations of inorganic elements in the exposed group were higher compared to those of the unexposed group. In this study, we observed that genetic damage, and change in oxidative balance were induced by the exposure of workers to complex mixtures of pesticides in the presence of inorganic compounds, whereby an infl uence of the genotype was evident.
publishDate 2016
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dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Anais da Academia Brasileira de Ciências. Rio de Janeiro. Vol. 88, n. 1 (mar. 2016), p. 349-360
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