Paracrine effects of bone marrow mononuclear cells in survival and cytokine expression after 90% partial hepatectomy
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/225839 |
Resumo: | Acute liver failure is a complex and fatal disease. Cell-based therapies are a promising alternative therapeutic approach for liver failure due to relatively simple technique and lower cost. The use of semipermeable microcapsules has become an interesting tool for evaluating paracrine effects in vivo. In this study, we aimed to assess the paracrine effects of bone marrow mononuclear cells (BMMC) encapsulated in sodium alginate to treat acute liver failure in an animal model of 90% partial hepatectomy (90% PH). Encapsulated BMMC were able to increase 10-day survival without enhancing liver regeneration markers. Gene expression of Il-6 and Il-10 in the remnant liver was markedly reduced at 6 h after 90% PH in animals receiving encapsulated BMMC compared to controls. This difference, however, was neither reflected by changes in the number of CD68+ cells nor by serum levels of IL6. On the other hand, treated animals presented increased caspase activity and gene expression in the liver. Taken together, these results suggest that BMMC regulate immune response and promote apoptosis in the liver after 90% PH by paracrine factors. These changes ultimately may be related to the higher survival observed in treated animals, suggesting that BMMC may be a promising alternative to treat acute liver failure. |
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Kieling, Carlos OscarCruz, Carolina UribeLópez, Mónica LujánOsvaldt, Betânia BerschSilveira, Themis Reverbel daMatte, Ursula da Silveira2021-08-18T04:41:14Z20171687-9678http://hdl.handle.net/10183/225839001101777Acute liver failure is a complex and fatal disease. Cell-based therapies are a promising alternative therapeutic approach for liver failure due to relatively simple technique and lower cost. The use of semipermeable microcapsules has become an interesting tool for evaluating paracrine effects in vivo. In this study, we aimed to assess the paracrine effects of bone marrow mononuclear cells (BMMC) encapsulated in sodium alginate to treat acute liver failure in an animal model of 90% partial hepatectomy (90% PH). Encapsulated BMMC were able to increase 10-day survival without enhancing liver regeneration markers. Gene expression of Il-6 and Il-10 in the remnant liver was markedly reduced at 6 h after 90% PH in animals receiving encapsulated BMMC compared to controls. This difference, however, was neither reflected by changes in the number of CD68+ cells nor by serum levels of IL6. On the other hand, treated animals presented increased caspase activity and gene expression in the liver. Taken together, these results suggest that BMMC regulate immune response and promote apoptosis in the liver after 90% PH by paracrine factors. These changes ultimately may be related to the higher survival observed in treated animals, suggesting that BMMC may be a promising alternative to treat acute liver failure.application/pdfengStem Cells International. London. Vol. 2017 (2017), Article ID 5270527 8 p.CitocinasHepatectomiaMedula ósseaParacrine effects of bone marrow mononuclear cells in survival and cytokine expression after 90% partial hepatectomyEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001101777.pdf.txt001101777.pdf.txtExtracted Texttext/plain34942http://www.lume.ufrgs.br/bitstream/10183/225839/2/001101777.pdf.txt3563148a2ef470ee94418fd37ece2506MD52ORIGINAL001101777.pdfTexto completo (inglês)application/pdf2597099http://www.lume.ufrgs.br/bitstream/10183/225839/1/001101777.pdf0fb13dd2241e7f0eae0db2ced9a7145cMD5110183/2258392021-08-18 05:20:33.784oai:www.lume.ufrgs.br:10183/225839Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-08-18T08:20:33Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Paracrine effects of bone marrow mononuclear cells in survival and cytokine expression after 90% partial hepatectomy |
title |
Paracrine effects of bone marrow mononuclear cells in survival and cytokine expression after 90% partial hepatectomy |
spellingShingle |
Paracrine effects of bone marrow mononuclear cells in survival and cytokine expression after 90% partial hepatectomy Kieling, Carlos Oscar Citocinas Hepatectomia Medula óssea |
title_short |
Paracrine effects of bone marrow mononuclear cells in survival and cytokine expression after 90% partial hepatectomy |
title_full |
Paracrine effects of bone marrow mononuclear cells in survival and cytokine expression after 90% partial hepatectomy |
title_fullStr |
Paracrine effects of bone marrow mononuclear cells in survival and cytokine expression after 90% partial hepatectomy |
title_full_unstemmed |
Paracrine effects of bone marrow mononuclear cells in survival and cytokine expression after 90% partial hepatectomy |
title_sort |
Paracrine effects of bone marrow mononuclear cells in survival and cytokine expression after 90% partial hepatectomy |
author |
Kieling, Carlos Oscar |
author_facet |
Kieling, Carlos Oscar Cruz, Carolina Uribe López, Mónica Luján Osvaldt, Betânia Bersch Silveira, Themis Reverbel da Matte, Ursula da Silveira |
author_role |
author |
author2 |
Cruz, Carolina Uribe López, Mónica Luján Osvaldt, Betânia Bersch Silveira, Themis Reverbel da Matte, Ursula da Silveira |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Kieling, Carlos Oscar Cruz, Carolina Uribe López, Mónica Luján Osvaldt, Betânia Bersch Silveira, Themis Reverbel da Matte, Ursula da Silveira |
dc.subject.por.fl_str_mv |
Citocinas Hepatectomia Medula óssea |
topic |
Citocinas Hepatectomia Medula óssea |
description |
Acute liver failure is a complex and fatal disease. Cell-based therapies are a promising alternative therapeutic approach for liver failure due to relatively simple technique and lower cost. The use of semipermeable microcapsules has become an interesting tool for evaluating paracrine effects in vivo. In this study, we aimed to assess the paracrine effects of bone marrow mononuclear cells (BMMC) encapsulated in sodium alginate to treat acute liver failure in an animal model of 90% partial hepatectomy (90% PH). Encapsulated BMMC were able to increase 10-day survival without enhancing liver regeneration markers. Gene expression of Il-6 and Il-10 in the remnant liver was markedly reduced at 6 h after 90% PH in animals receiving encapsulated BMMC compared to controls. This difference, however, was neither reflected by changes in the number of CD68+ cells nor by serum levels of IL6. On the other hand, treated animals presented increased caspase activity and gene expression in the liver. Taken together, these results suggest that BMMC regulate immune response and promote apoptosis in the liver after 90% PH by paracrine factors. These changes ultimately may be related to the higher survival observed in treated animals, suggesting that BMMC may be a promising alternative to treat acute liver failure. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2021-08-18T04:41:14Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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http://hdl.handle.net/10183/225839 |
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001101777 |
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eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Stem Cells International. London. Vol. 2017 (2017), Article ID 5270527 8 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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