Association between molecular markers and behavioral phenotypes in the immatures of a butterfly
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/183939 |
Resumo: | Newly hatched caterpillars of the butterfly Heliconius erato phyllis routinely cannibalize eggs. In a manifestation of kin recognition they cannibalize sibling eggs less frequently than unrelated eggs. Previous work has estimated the heritability of kin recognition in H. erato phyllis to lie between 14 and 48%. It has furthermore been shown that the inheritance of kin recognition is compatible with a quantitative model with a threshold. Here we present the results of a preliminary study, in which we tested for associations between behavioral kin recognition phenotypes and AFLP and SSR markers. We implemented two experimental approaches: (1) a cannibalism test using sibling eggs only, which allowed for only two behavioral outcomes (cannibal and non-cannibal), and (2) a cannibalism test using two sibling eggs and one unrelated egg, which allowed four outcomes [cannibal who does not recognize siblings, cannibal who recognizes siblings, “super-cannibal” (cannibal of both eggs), and “super non-cannibal” (does not cannibalize eggs at all)]. Single-marker analyses were performed using 2 tests and logistic regression with null markers as covariates. Results of the 2 tests identified 72 associations for experimental design 1 and 73 associations for design 2. Logistic regression analysis of the markers found to be significant in the 2 test resulted in 20 associations for design 1 and 11 associations for design 2. Experiment 2 identified markers that were more frequently present or absent in cannibals who recognize siblings and super non-cannibals; i.e. in both phenotypes capable of kin recognition. |
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Nardin, Janaína deBuffon, VanessaRevers, Luis FernandoAraujo, Aldo Mellender de2018-10-24T02:43:49Z20181415-4757http://hdl.handle.net/10183/183939001073362Newly hatched caterpillars of the butterfly Heliconius erato phyllis routinely cannibalize eggs. In a manifestation of kin recognition they cannibalize sibling eggs less frequently than unrelated eggs. Previous work has estimated the heritability of kin recognition in H. erato phyllis to lie between 14 and 48%. It has furthermore been shown that the inheritance of kin recognition is compatible with a quantitative model with a threshold. Here we present the results of a preliminary study, in which we tested for associations between behavioral kin recognition phenotypes and AFLP and SSR markers. We implemented two experimental approaches: (1) a cannibalism test using sibling eggs only, which allowed for only two behavioral outcomes (cannibal and non-cannibal), and (2) a cannibalism test using two sibling eggs and one unrelated egg, which allowed four outcomes [cannibal who does not recognize siblings, cannibal who recognizes siblings, “super-cannibal” (cannibal of both eggs), and “super non-cannibal” (does not cannibalize eggs at all)]. Single-marker analyses were performed using 2 tests and logistic regression with null markers as covariates. Results of the 2 tests identified 72 associations for experimental design 1 and 73 associations for design 2. Logistic regression analysis of the markers found to be significant in the 2 test resulted in 20 associations for design 1 and 11 associations for design 2. Experiment 2 identified markers that were more frequently present or absent in cannibals who recognize siblings and super non-cannibals; i.e. in both phenotypes capable of kin recognition.application/pdfengGenetics and molecular biology. Ribeirão Preto, SP. Vol. 41, no. 1 suppl (mar. 2018), p. 243-252.LepidopteraHeliconius erato phyllisCanibalismoKin discriminationCaterpillar-egg cannibalismLepidopteraHeliconiusAFLPAssociation between molecular markers and behavioral phenotypes in the immatures of a butterflyinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001073362.pdfTexto completo (inglês)application/pdf476306http://www.lume.ufrgs.br/bitstream/10183/183939/1/001073362.pdf0f253895f97b0c8d3734d19167414a58MD51TEXT001073362.pdf.txt001073362.pdf.txtExtracted Texttext/plain46542http://www.lume.ufrgs.br/bitstream/10183/183939/2/001073362.pdf.txtbc97a56dbbbd9c78e929b085e5655716MD52THUMBNAIL001073362.pdf.jpg001073362.pdf.jpgGenerated Thumbnailimage/jpeg1872http://www.lume.ufrgs.br/bitstream/10183/183939/3/001073362.pdf.jpg797e1986971f90058861d6ec0a784d3dMD5310183/1839392019-11-03 03:52:04.93441oai:www.lume.ufrgs.br:10183/183939Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-11-03T05:52:04Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Association between molecular markers and behavioral phenotypes in the immatures of a butterfly |
title |
Association between molecular markers and behavioral phenotypes in the immatures of a butterfly |
spellingShingle |
Association between molecular markers and behavioral phenotypes in the immatures of a butterfly Nardin, Janaína de Lepidoptera Heliconius erato phyllis Canibalismo Kin discrimination Caterpillar-egg cannibalism Lepidoptera Heliconius AFLP |
title_short |
Association between molecular markers and behavioral phenotypes in the immatures of a butterfly |
title_full |
Association between molecular markers and behavioral phenotypes in the immatures of a butterfly |
title_fullStr |
Association between molecular markers and behavioral phenotypes in the immatures of a butterfly |
title_full_unstemmed |
Association between molecular markers and behavioral phenotypes in the immatures of a butterfly |
title_sort |
Association between molecular markers and behavioral phenotypes in the immatures of a butterfly |
author |
Nardin, Janaína de |
author_facet |
Nardin, Janaína de Buffon, Vanessa Revers, Luis Fernando Araujo, Aldo Mellender de |
author_role |
author |
author2 |
Buffon, Vanessa Revers, Luis Fernando Araujo, Aldo Mellender de |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Nardin, Janaína de Buffon, Vanessa Revers, Luis Fernando Araujo, Aldo Mellender de |
dc.subject.por.fl_str_mv |
Lepidoptera Heliconius erato phyllis Canibalismo |
topic |
Lepidoptera Heliconius erato phyllis Canibalismo Kin discrimination Caterpillar-egg cannibalism Lepidoptera Heliconius AFLP |
dc.subject.eng.fl_str_mv |
Kin discrimination Caterpillar-egg cannibalism Lepidoptera Heliconius AFLP |
description |
Newly hatched caterpillars of the butterfly Heliconius erato phyllis routinely cannibalize eggs. In a manifestation of kin recognition they cannibalize sibling eggs less frequently than unrelated eggs. Previous work has estimated the heritability of kin recognition in H. erato phyllis to lie between 14 and 48%. It has furthermore been shown that the inheritance of kin recognition is compatible with a quantitative model with a threshold. Here we present the results of a preliminary study, in which we tested for associations between behavioral kin recognition phenotypes and AFLP and SSR markers. We implemented two experimental approaches: (1) a cannibalism test using sibling eggs only, which allowed for only two behavioral outcomes (cannibal and non-cannibal), and (2) a cannibalism test using two sibling eggs and one unrelated egg, which allowed four outcomes [cannibal who does not recognize siblings, cannibal who recognizes siblings, “super-cannibal” (cannibal of both eggs), and “super non-cannibal” (does not cannibalize eggs at all)]. Single-marker analyses were performed using 2 tests and logistic regression with null markers as covariates. Results of the 2 tests identified 72 associations for experimental design 1 and 73 associations for design 2. Logistic regression analysis of the markers found to be significant in the 2 test resulted in 20 associations for design 1 and 11 associations for design 2. Experiment 2 identified markers that were more frequently present or absent in cannibals who recognize siblings and super non-cannibals; i.e. in both phenotypes capable of kin recognition. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-10-24T02:43:49Z |
dc.date.issued.fl_str_mv |
2018 |
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info:eu-repo/semantics/article info:eu-repo/semantics/other |
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http://hdl.handle.net/10183/183939 |
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1415-4757 |
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001073362 |
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http://hdl.handle.net/10183/183939 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Genetics and molecular biology. Ribeirão Preto, SP. Vol. 41, no. 1 suppl (mar. 2018), p. 243-252. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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