Detection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/30534 |
Resumo: | The introduction of newer molecular methods has led to the discovery of new respiratory viruses, such as human metapneumovirus (hMPV) and human bocavirus (hBoV), in respiratory tract specimens. We have studied the occurrence of hMPV and hBoV in the Porto Alegre (PA) metropolitan area, one of the southernmost cities of Brazil, evaluating children with suspected lower respiratory tract infection from May 2007-June 2008. A real-time polymerase chain reaction method was used for amplification and detection of hMPV and hBoV and to evaluate coinfections with respiratory syncytial virus (RSV), influenza A and B, parainfluenza 1, 2 and 3, human rhinovirus and human adenovirus. Of the 455 nasopharyngeal aspirates tested, hMPV was detected in 14.5% of samples and hBoV in 13.2%. A unique causative viral agent was identified in 46.2% samples and the coinfection rate was 43.7%. For hBoV, 98.3% of all positive samples were from patients with mixed infections. Similarly, 84.8% of all hMPV-positive results were also observed in mixed infections. Both hBoV and hMPV usually appeared with RSV. In summary, this is the first confirmation that hMPV and hBoV circulate in PA; this provides evidence of frequent involvement of both viruses in children with clinical signs of acute viral respiratory tract infection, although they mainly appeared as coinfection agents. |
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Pilger, Diogo AndreCantarelli, Vlademir VicenteAmantea, Sergio LuisLeistner-Segal, Sandra2011-08-04T06:01:09Z20110074-0276http://hdl.handle.net/10183/30534000767028The introduction of newer molecular methods has led to the discovery of new respiratory viruses, such as human metapneumovirus (hMPV) and human bocavirus (hBoV), in respiratory tract specimens. We have studied the occurrence of hMPV and hBoV in the Porto Alegre (PA) metropolitan area, one of the southernmost cities of Brazil, evaluating children with suspected lower respiratory tract infection from May 2007-June 2008. A real-time polymerase chain reaction method was used for amplification and detection of hMPV and hBoV and to evaluate coinfections with respiratory syncytial virus (RSV), influenza A and B, parainfluenza 1, 2 and 3, human rhinovirus and human adenovirus. Of the 455 nasopharyngeal aspirates tested, hMPV was detected in 14.5% of samples and hBoV in 13.2%. A unique causative viral agent was identified in 46.2% samples and the coinfection rate was 43.7%. For hBoV, 98.3% of all positive samples were from patients with mixed infections. Similarly, 84.8% of all hMPV-positive results were also observed in mixed infections. Both hBoV and hMPV usually appeared with RSV. In summary, this is the first confirmation that hMPV and hBoV circulate in PA; this provides evidence of frequent involvement of both viruses in children with clinical signs of acute viral respiratory tract infection, although they mainly appeared as coinfection agents.application/pdfengMemórias do Instituto Oswaldo Cruz : an international journal of biological and biomedical research. Vol. 106, n. 1 (Feb. 2011), p. 56-60MetapneumovirusBocavirusInfecções respiratóriasHuman metapneumovirusHuman bocavirusReal-time PCRRespiratory tract infectionDetection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern Brazilinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000767028.pdf000767028.pdfTexto completo (inglês)application/pdf384179http://www.lume.ufrgs.br/bitstream/10183/30534/1/000767028.pdf2b846ff5ea443891e2757ab349e17059MD51TEXT000767028.pdf.txt000767028.pdf.txtExtracted Texttext/plain25220http://www.lume.ufrgs.br/bitstream/10183/30534/2/000767028.pdf.txt6ccf702263c1059ab7e9ff20ed030d9eMD52THUMBNAIL000767028.pdf.jpg000767028.pdf.jpgGenerated Thumbnailimage/jpeg2182http://www.lume.ufrgs.br/bitstream/10183/30534/3/000767028.pdf.jpg4b28967102732a3f37c7aff980a22bc5MD5310183/305342018-10-30 07:52:17.657oai:www.lume.ufrgs.br:10183/30534Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-30T10:52:17Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Detection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern Brazil |
title |
Detection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern Brazil |
spellingShingle |
Detection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern Brazil Pilger, Diogo Andre Metapneumovirus Bocavirus Infecções respiratórias Human metapneumovirus Human bocavirus Real-time PCR Respiratory tract infection |
title_short |
Detection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern Brazil |
title_full |
Detection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern Brazil |
title_fullStr |
Detection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern Brazil |
title_full_unstemmed |
Detection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern Brazil |
title_sort |
Detection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern Brazil |
author |
Pilger, Diogo Andre |
author_facet |
Pilger, Diogo Andre Cantarelli, Vlademir Vicente Amantea, Sergio Luis Leistner-Segal, Sandra |
author_role |
author |
author2 |
Cantarelli, Vlademir Vicente Amantea, Sergio Luis Leistner-Segal, Sandra |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Pilger, Diogo Andre Cantarelli, Vlademir Vicente Amantea, Sergio Luis Leistner-Segal, Sandra |
dc.subject.por.fl_str_mv |
Metapneumovirus Bocavirus Infecções respiratórias |
topic |
Metapneumovirus Bocavirus Infecções respiratórias Human metapneumovirus Human bocavirus Real-time PCR Respiratory tract infection |
dc.subject.eng.fl_str_mv |
Human metapneumovirus Human bocavirus Real-time PCR Respiratory tract infection |
description |
The introduction of newer molecular methods has led to the discovery of new respiratory viruses, such as human metapneumovirus (hMPV) and human bocavirus (hBoV), in respiratory tract specimens. We have studied the occurrence of hMPV and hBoV in the Porto Alegre (PA) metropolitan area, one of the southernmost cities of Brazil, evaluating children with suspected lower respiratory tract infection from May 2007-June 2008. A real-time polymerase chain reaction method was used for amplification and detection of hMPV and hBoV and to evaluate coinfections with respiratory syncytial virus (RSV), influenza A and B, parainfluenza 1, 2 and 3, human rhinovirus and human adenovirus. Of the 455 nasopharyngeal aspirates tested, hMPV was detected in 14.5% of samples and hBoV in 13.2%. A unique causative viral agent was identified in 46.2% samples and the coinfection rate was 43.7%. For hBoV, 98.3% of all positive samples were from patients with mixed infections. Similarly, 84.8% of all hMPV-positive results were also observed in mixed infections. Both hBoV and hMPV usually appeared with RSV. In summary, this is the first confirmation that hMPV and hBoV circulate in PA; this provides evidence of frequent involvement of both viruses in children with clinical signs of acute viral respiratory tract infection, although they mainly appeared as coinfection agents. |
publishDate |
2011 |
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2011-08-04T06:01:09Z |
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2011 |
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dc.language.iso.fl_str_mv |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Memórias do Instituto Oswaldo Cruz : an international journal of biological and biomedical research. Vol. 106, n. 1 (Feb. 2011), p. 56-60 |
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