Detection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern Brazil

Detalhes bibliográficos
Autor(a) principal: Pilger, Diogo Andre
Data de Publicação: 2011
Outros Autores: Cantarelli, Vlademir Vicente, Amantea, Sergio Luis, Leistner-Segal, Sandra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/30534
Resumo: The introduction of newer molecular methods has led to the discovery of new respiratory viruses, such as human metapneumovirus (hMPV) and human bocavirus (hBoV), in respiratory tract specimens. We have studied the occurrence of hMPV and hBoV in the Porto Alegre (PA) metropolitan area, one of the southernmost cities of Brazil, evaluating children with suspected lower respiratory tract infection from May 2007-June 2008. A real-time polymerase chain reaction method was used for amplification and detection of hMPV and hBoV and to evaluate coinfections with respiratory syncytial virus (RSV), influenza A and B, parainfluenza 1, 2 and 3, human rhinovirus and human adenovirus. Of the 455 nasopharyngeal aspirates tested, hMPV was detected in 14.5% of samples and hBoV in 13.2%. A unique causative viral agent was identified in 46.2% samples and the coinfection rate was 43.7%. For hBoV, 98.3% of all positive samples were from patients with mixed infections. Similarly, 84.8% of all hMPV-positive results were also observed in mixed infections. Both hBoV and hMPV usually appeared with RSV. In summary, this is the first confirmation that hMPV and hBoV circulate in PA; this provides evidence of frequent involvement of both viruses in children with clinical signs of acute viral respiratory tract infection, although they mainly appeared as coinfection agents.
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spelling Pilger, Diogo AndreCantarelli, Vlademir VicenteAmantea, Sergio LuisLeistner-Segal, Sandra2011-08-04T06:01:09Z20110074-0276http://hdl.handle.net/10183/30534000767028The introduction of newer molecular methods has led to the discovery of new respiratory viruses, such as human metapneumovirus (hMPV) and human bocavirus (hBoV), in respiratory tract specimens. We have studied the occurrence of hMPV and hBoV in the Porto Alegre (PA) metropolitan area, one of the southernmost cities of Brazil, evaluating children with suspected lower respiratory tract infection from May 2007-June 2008. A real-time polymerase chain reaction method was used for amplification and detection of hMPV and hBoV and to evaluate coinfections with respiratory syncytial virus (RSV), influenza A and B, parainfluenza 1, 2 and 3, human rhinovirus and human adenovirus. Of the 455 nasopharyngeal aspirates tested, hMPV was detected in 14.5% of samples and hBoV in 13.2%. A unique causative viral agent was identified in 46.2% samples and the coinfection rate was 43.7%. For hBoV, 98.3% of all positive samples were from patients with mixed infections. Similarly, 84.8% of all hMPV-positive results were also observed in mixed infections. Both hBoV and hMPV usually appeared with RSV. In summary, this is the first confirmation that hMPV and hBoV circulate in PA; this provides evidence of frequent involvement of both viruses in children with clinical signs of acute viral respiratory tract infection, although they mainly appeared as coinfection agents.application/pdfengMemórias do Instituto Oswaldo Cruz : an international journal of biological and biomedical research. Vol. 106, n. 1 (Feb. 2011), p. 56-60MetapneumovirusBocavirusInfecções respiratóriasHuman metapneumovirusHuman bocavirusReal-time PCRRespiratory tract infectionDetection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern Brazilinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000767028.pdf000767028.pdfTexto completo (inglês)application/pdf384179http://www.lume.ufrgs.br/bitstream/10183/30534/1/000767028.pdf2b846ff5ea443891e2757ab349e17059MD51TEXT000767028.pdf.txt000767028.pdf.txtExtracted Texttext/plain25220http://www.lume.ufrgs.br/bitstream/10183/30534/2/000767028.pdf.txt6ccf702263c1059ab7e9ff20ed030d9eMD52THUMBNAIL000767028.pdf.jpg000767028.pdf.jpgGenerated Thumbnailimage/jpeg2182http://www.lume.ufrgs.br/bitstream/10183/30534/3/000767028.pdf.jpg4b28967102732a3f37c7aff980a22bc5MD5310183/305342018-10-30 07:52:17.657oai:www.lume.ufrgs.br:10183/30534Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-30T10:52:17Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Detection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern Brazil
title Detection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern Brazil
spellingShingle Detection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern Brazil
Pilger, Diogo Andre
Metapneumovirus
Bocavirus
Infecções respiratórias
Human metapneumovirus
Human bocavirus
Real-time PCR
Respiratory tract infection
title_short Detection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern Brazil
title_full Detection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern Brazil
title_fullStr Detection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern Brazil
title_full_unstemmed Detection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern Brazil
title_sort Detection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern Brazil
author Pilger, Diogo Andre
author_facet Pilger, Diogo Andre
Cantarelli, Vlademir Vicente
Amantea, Sergio Luis
Leistner-Segal, Sandra
author_role author
author2 Cantarelli, Vlademir Vicente
Amantea, Sergio Luis
Leistner-Segal, Sandra
author2_role author
author
author
dc.contributor.author.fl_str_mv Pilger, Diogo Andre
Cantarelli, Vlademir Vicente
Amantea, Sergio Luis
Leistner-Segal, Sandra
dc.subject.por.fl_str_mv Metapneumovirus
Bocavirus
Infecções respiratórias
topic Metapneumovirus
Bocavirus
Infecções respiratórias
Human metapneumovirus
Human bocavirus
Real-time PCR
Respiratory tract infection
dc.subject.eng.fl_str_mv Human metapneumovirus
Human bocavirus
Real-time PCR
Respiratory tract infection
description The introduction of newer molecular methods has led to the discovery of new respiratory viruses, such as human metapneumovirus (hMPV) and human bocavirus (hBoV), in respiratory tract specimens. We have studied the occurrence of hMPV and hBoV in the Porto Alegre (PA) metropolitan area, one of the southernmost cities of Brazil, evaluating children with suspected lower respiratory tract infection from May 2007-June 2008. A real-time polymerase chain reaction method was used for amplification and detection of hMPV and hBoV and to evaluate coinfections with respiratory syncytial virus (RSV), influenza A and B, parainfluenza 1, 2 and 3, human rhinovirus and human adenovirus. Of the 455 nasopharyngeal aspirates tested, hMPV was detected in 14.5% of samples and hBoV in 13.2%. A unique causative viral agent was identified in 46.2% samples and the coinfection rate was 43.7%. For hBoV, 98.3% of all positive samples were from patients with mixed infections. Similarly, 84.8% of all hMPV-positive results were also observed in mixed infections. Both hBoV and hMPV usually appeared with RSV. In summary, this is the first confirmation that hMPV and hBoV circulate in PA; this provides evidence of frequent involvement of both viruses in children with clinical signs of acute viral respiratory tract infection, although they mainly appeared as coinfection agents.
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dc.relation.ispartof.pt_BR.fl_str_mv Memórias do Instituto Oswaldo Cruz : an international journal of biological and biomedical research. Vol. 106, n. 1 (Feb. 2011), p. 56-60
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