Effect of Helicobacter pylori on NFKB1, p38α and TNF-α mRNA expression levels in human gastric mucosa
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/172846 |
Resumo: | Helicobacter pylori infects ~50% of the world population, causing chronic gastritis and other forms of cellular damage. The present study assessed the influence of H. pylori on the mRNA expression levels of nuclear factor κB1 (NFKB1), p38α and tumor necrosis factor α (TNF α) in human gastric mucosa in a southern Brazilian population. Human gastric tissue was collected by upper endoscopy and H. pylori diagnosis was performed using a rapid urease test and histological analysis. Total RNA was extracted and purified for subsequent cDNA synthesis and analysis by quantitative polymerase chain reaction (qPCR). The gastric tissue samples were divided into four groups as follows: Normal, inactive chronic gastritis, active chronic gastritis and intestinal metaplasia. The SDHA gene was classified as the most stable when compared with ACTB, GAPDH, B2M and HPRT1 genes, and was therefore selected as the reference gene for qPCR data normalization. TNF α mRNA expression was significantly higher in samples that were positive for H. pylori and with active chronic gastritis. However, no difference was detected in the mRNA expression levels of NFKB1 and p38α between the groups. The present study concluded that the presence of H. pylori is associated with TNF α upregulation in human gastric mucosa, but had no effect on NFKB1 and p38α mRNA expression levels. |
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Oliveira, Henrique Sulzbach deBiolchi, VanderleiMedeiros, Helouise RichardtJantsch, Daiane Bizerra GandorDelving, Luciana Knabben de Oliveira BeckerReckziegel, RobertoGoettert, Marcia InesBrum, Ilma SimoniPozzobon, Adriane2018-02-24T02:26:04Z20161792-0981http://hdl.handle.net/10183/172846001059255Helicobacter pylori infects ~50% of the world population, causing chronic gastritis and other forms of cellular damage. The present study assessed the influence of H. pylori on the mRNA expression levels of nuclear factor κB1 (NFKB1), p38α and tumor necrosis factor α (TNF α) in human gastric mucosa in a southern Brazilian population. Human gastric tissue was collected by upper endoscopy and H. pylori diagnosis was performed using a rapid urease test and histological analysis. Total RNA was extracted and purified for subsequent cDNA synthesis and analysis by quantitative polymerase chain reaction (qPCR). The gastric tissue samples were divided into four groups as follows: Normal, inactive chronic gastritis, active chronic gastritis and intestinal metaplasia. The SDHA gene was classified as the most stable when compared with ACTB, GAPDH, B2M and HPRT1 genes, and was therefore selected as the reference gene for qPCR data normalization. TNF α mRNA expression was significantly higher in samples that were positive for H. pylori and with active chronic gastritis. However, no difference was detected in the mRNA expression levels of NFKB1 and p38α between the groups. The present study concluded that the presence of H. pylori is associated with TNF α upregulation in human gastric mucosa, but had no effect on NFKB1 and p38α mRNA expression levels.application/pdfengExperimental and therapeutic medicine. Athens. Vol. 11, no. 6 (Jun. 2016), p. 2365-2372Expressão gênicaHelicobacter pyloriGastriteInflamaçãoEffect of Helicobacter pylori on NFKB1, p38α and TNF-α mRNA expression levels in human gastric mucosaEffect of Helicobacter pylori on NFKB1, p38alpha and TNF-alpha mRNA expression levels in human gastric mucosa Estrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001059255.pdf001059255.pdfTexto completo (inglês)application/pdf733039http://www.lume.ufrgs.br/bitstream/10183/172846/1/001059255.pdf8edec7285d46afc39ec6d1de6d205738MD51TEXT001059255.pdf.txt001059255.pdf.txtExtracted Texttext/plain42025http://www.lume.ufrgs.br/bitstream/10183/172846/2/001059255.pdf.txt1504ac2163bbe72c75733b87ef1fdd4dMD52THUMBNAIL001059255.pdf.jpg001059255.pdf.jpgGenerated Thumbnailimage/jpeg2269http://www.lume.ufrgs.br/bitstream/10183/172846/3/001059255.pdf.jpga060d750ada1bb72a32ec5fe77736016MD5310183/1728462018-10-29 08:51:47.391oai:www.lume.ufrgs.br:10183/172846Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-29T11:51:47Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Effect of Helicobacter pylori on NFKB1, p38α and TNF-α mRNA expression levels in human gastric mucosa |
dc.title.alternative.en.fl_str_mv |
Effect of Helicobacter pylori on NFKB1, p38alpha and TNF-alpha mRNA expression levels in human gastric mucosa |
title |
Effect of Helicobacter pylori on NFKB1, p38α and TNF-α mRNA expression levels in human gastric mucosa |
spellingShingle |
Effect of Helicobacter pylori on NFKB1, p38α and TNF-α mRNA expression levels in human gastric mucosa Oliveira, Henrique Sulzbach de Expressão gênica Helicobacter pylori Gastrite Inflamação |
title_short |
Effect of Helicobacter pylori on NFKB1, p38α and TNF-α mRNA expression levels in human gastric mucosa |
title_full |
Effect of Helicobacter pylori on NFKB1, p38α and TNF-α mRNA expression levels in human gastric mucosa |
title_fullStr |
Effect of Helicobacter pylori on NFKB1, p38α and TNF-α mRNA expression levels in human gastric mucosa |
title_full_unstemmed |
Effect of Helicobacter pylori on NFKB1, p38α and TNF-α mRNA expression levels in human gastric mucosa |
title_sort |
Effect of Helicobacter pylori on NFKB1, p38α and TNF-α mRNA expression levels in human gastric mucosa |
author |
Oliveira, Henrique Sulzbach de |
author_facet |
Oliveira, Henrique Sulzbach de Biolchi, Vanderlei Medeiros, Helouise Richardt Jantsch, Daiane Bizerra Gandor Delving, Luciana Knabben de Oliveira Becker Reckziegel, Roberto Goettert, Marcia Ines Brum, Ilma Simoni Pozzobon, Adriane |
author_role |
author |
author2 |
Biolchi, Vanderlei Medeiros, Helouise Richardt Jantsch, Daiane Bizerra Gandor Delving, Luciana Knabben de Oliveira Becker Reckziegel, Roberto Goettert, Marcia Ines Brum, Ilma Simoni Pozzobon, Adriane |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Henrique Sulzbach de Biolchi, Vanderlei Medeiros, Helouise Richardt Jantsch, Daiane Bizerra Gandor Delving, Luciana Knabben de Oliveira Becker Reckziegel, Roberto Goettert, Marcia Ines Brum, Ilma Simoni Pozzobon, Adriane |
dc.subject.por.fl_str_mv |
Expressão gênica Helicobacter pylori Gastrite Inflamação |
topic |
Expressão gênica Helicobacter pylori Gastrite Inflamação |
description |
Helicobacter pylori infects ~50% of the world population, causing chronic gastritis and other forms of cellular damage. The present study assessed the influence of H. pylori on the mRNA expression levels of nuclear factor κB1 (NFKB1), p38α and tumor necrosis factor α (TNF α) in human gastric mucosa in a southern Brazilian population. Human gastric tissue was collected by upper endoscopy and H. pylori diagnosis was performed using a rapid urease test and histological analysis. Total RNA was extracted and purified for subsequent cDNA synthesis and analysis by quantitative polymerase chain reaction (qPCR). The gastric tissue samples were divided into four groups as follows: Normal, inactive chronic gastritis, active chronic gastritis and intestinal metaplasia. The SDHA gene was classified as the most stable when compared with ACTB, GAPDH, B2M and HPRT1 genes, and was therefore selected as the reference gene for qPCR data normalization. TNF α mRNA expression was significantly higher in samples that were positive for H. pylori and with active chronic gastritis. However, no difference was detected in the mRNA expression levels of NFKB1 and p38α between the groups. The present study concluded that the presence of H. pylori is associated with TNF α upregulation in human gastric mucosa, but had no effect on NFKB1 and p38α mRNA expression levels. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2018-02-24T02:26:04Z |
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1792-0981 |
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001059255 |
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http://hdl.handle.net/10183/172846 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Experimental and therapeutic medicine. Athens. Vol. 11, no. 6 (Jun. 2016), p. 2365-2372 |
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openAccess |
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