Combined effects of melatonin and topical hypothermia on renal ischemia-reperfusion injury in rats
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/202107 |
Resumo: | Purpose: To evaluate whether their combination was more effective than either alone in decreasing renal damage due to ischemia/reperfusion (I/R) injury in rats. Methods: Thirty-two Wistar rats were assigned to four groups. Following right nephrectomy, their left kidneys were subjected to warm ischemia (IR), cold ischemia (TH+IR), intraperitoneal injection of 10 mg/kg melatonin (MEL+IR), or injection of 10 mg/kg melatonin followed by cold ischemia (MEL+TH+IR). Eight randomly assigned right kidneys constituted the control group. After 240 min of reperfusion, left nephrectomy was performed for histopathological evaluation, lipid peroxidation, and measurement of antioxidant enzyme activity. Serum was collected to measure urea and creatinine concentrations. Results: Histopathological damage induced by ischemia and reperfusion was more attenuated in the MEL+TH+IR group than in the MEL+IR and TH+IR groups (p<0.037). Superoxide dismutase activity was significantly higher (p<0.029) and creatinine (p<0.001) and urea (p<0.001) concentrations were significantly lower in the MEL+TH+IR group than in the MEL+IR and TH+IR groups. Conclusion: The combination of melatonin (MEL) and topical hypothermia (TH) better protects against renal I/R injury than does MEL or TH alone. |
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Souza, Pablo CambesesSantos, Emanuel Burck dosMotta, Guilherme LangBona, Silvia ReginaSchaefer, Pedro GuilhermeCampagnol, DanielaBortolini, TiagoCorso, Carlos Otavio2019-11-27T04:02:29Z20180102-8650http://hdl.handle.net/10183/202107001105939Purpose: To evaluate whether their combination was more effective than either alone in decreasing renal damage due to ischemia/reperfusion (I/R) injury in rats. Methods: Thirty-two Wistar rats were assigned to four groups. Following right nephrectomy, their left kidneys were subjected to warm ischemia (IR), cold ischemia (TH+IR), intraperitoneal injection of 10 mg/kg melatonin (MEL+IR), or injection of 10 mg/kg melatonin followed by cold ischemia (MEL+TH+IR). Eight randomly assigned right kidneys constituted the control group. After 240 min of reperfusion, left nephrectomy was performed for histopathological evaluation, lipid peroxidation, and measurement of antioxidant enzyme activity. Serum was collected to measure urea and creatinine concentrations. Results: Histopathological damage induced by ischemia and reperfusion was more attenuated in the MEL+TH+IR group than in the MEL+IR and TH+IR groups (p<0.037). Superoxide dismutase activity was significantly higher (p<0.029) and creatinine (p<0.001) and urea (p<0.001) concentrations were significantly lower in the MEL+TH+IR group than in the MEL+IR and TH+IR groups. Conclusion: The combination of melatonin (MEL) and topical hypothermia (TH) better protects against renal I/R injury than does MEL or TH alone.application/pdfengActa cirúrgica brasileira. São Paulo. vol. 33, n. 3 (mar. 2018), p. 197-206MelatoninaHipotermiaTraumatismo por reperfusãoEstresse oxidativoRimMelatoninHypothermiaReperfusion injuryOxidative stressKidneyRatsCombined effects of melatonin and topical hypothermia on renal ischemia-reperfusion injury in ratsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001105939.pdf.txt001105939.pdf.txtExtracted Texttext/plain31524http://www.lume.ufrgs.br/bitstream/10183/202107/2/001105939.pdf.txt55b1d1a06223a7965351e895235e2c6cMD52ORIGINAL001105939.pdfTexto completo (inglês)application/pdf908229http://www.lume.ufrgs.br/bitstream/10183/202107/1/001105939.pdf21d18d04d5fd130ab3fee8e4e70a85cdMD5110183/2021072019-11-28 04:58:43.67836oai:www.lume.ufrgs.br:10183/202107Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-11-28T06:58:43Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Combined effects of melatonin and topical hypothermia on renal ischemia-reperfusion injury in rats |
title |
Combined effects of melatonin and topical hypothermia on renal ischemia-reperfusion injury in rats |
spellingShingle |
Combined effects of melatonin and topical hypothermia on renal ischemia-reperfusion injury in rats Souza, Pablo Cambeses Melatonina Hipotermia Traumatismo por reperfusão Estresse oxidativo Rim Melatonin Hypothermia Reperfusion injury Oxidative stress Kidney Rats |
title_short |
Combined effects of melatonin and topical hypothermia on renal ischemia-reperfusion injury in rats |
title_full |
Combined effects of melatonin and topical hypothermia on renal ischemia-reperfusion injury in rats |
title_fullStr |
Combined effects of melatonin and topical hypothermia on renal ischemia-reperfusion injury in rats |
title_full_unstemmed |
Combined effects of melatonin and topical hypothermia on renal ischemia-reperfusion injury in rats |
title_sort |
Combined effects of melatonin and topical hypothermia on renal ischemia-reperfusion injury in rats |
author |
Souza, Pablo Cambeses |
author_facet |
Souza, Pablo Cambeses Santos, Emanuel Burck dos Motta, Guilherme Lang Bona, Silvia Regina Schaefer, Pedro Guilherme Campagnol, Daniela Bortolini, Tiago Corso, Carlos Otavio |
author_role |
author |
author2 |
Santos, Emanuel Burck dos Motta, Guilherme Lang Bona, Silvia Regina Schaefer, Pedro Guilherme Campagnol, Daniela Bortolini, Tiago Corso, Carlos Otavio |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Souza, Pablo Cambeses Santos, Emanuel Burck dos Motta, Guilherme Lang Bona, Silvia Regina Schaefer, Pedro Guilherme Campagnol, Daniela Bortolini, Tiago Corso, Carlos Otavio |
dc.subject.por.fl_str_mv |
Melatonina Hipotermia Traumatismo por reperfusão Estresse oxidativo Rim |
topic |
Melatonina Hipotermia Traumatismo por reperfusão Estresse oxidativo Rim Melatonin Hypothermia Reperfusion injury Oxidative stress Kidney Rats |
dc.subject.eng.fl_str_mv |
Melatonin Hypothermia Reperfusion injury Oxidative stress Kidney Rats |
description |
Purpose: To evaluate whether their combination was more effective than either alone in decreasing renal damage due to ischemia/reperfusion (I/R) injury in rats. Methods: Thirty-two Wistar rats were assigned to four groups. Following right nephrectomy, their left kidneys were subjected to warm ischemia (IR), cold ischemia (TH+IR), intraperitoneal injection of 10 mg/kg melatonin (MEL+IR), or injection of 10 mg/kg melatonin followed by cold ischemia (MEL+TH+IR). Eight randomly assigned right kidneys constituted the control group. After 240 min of reperfusion, left nephrectomy was performed for histopathological evaluation, lipid peroxidation, and measurement of antioxidant enzyme activity. Serum was collected to measure urea and creatinine concentrations. Results: Histopathological damage induced by ischemia and reperfusion was more attenuated in the MEL+TH+IR group than in the MEL+IR and TH+IR groups (p<0.037). Superoxide dismutase activity was significantly higher (p<0.029) and creatinine (p<0.001) and urea (p<0.001) concentrations were significantly lower in the MEL+TH+IR group than in the MEL+IR and TH+IR groups. Conclusion: The combination of melatonin (MEL) and topical hypothermia (TH) better protects against renal I/R injury than does MEL or TH alone. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2019-11-27T04:02:29Z |
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http://hdl.handle.net/10183/202107 |
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0102-8650 |
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http://hdl.handle.net/10183/202107 |
dc.language.iso.fl_str_mv |
eng |
language |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Acta cirúrgica brasileira. São Paulo. vol. 33, n. 3 (mar. 2018), p. 197-206 |
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info:eu-repo/semantics/openAccess |
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