Tick-host range adaptation : changes in protein profiles in unfed adult Ixodes scapularis and Amblyomma americanum saliva stimulated to feed on different hosts

Detalhes bibliográficos
Autor(a) principal: Tirloni, Lucas
Data de Publicação: 2017
Outros Autores: Kim, Tae Kwon, Pinto, Antonio Frederico Michel, Yates III, John R., Vaz Junior, Itabajara da Silva, Mulenga, Albert
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/173336
Resumo: Understanding the molecular basis of how ticks adapt to feed on different animal hosts is central to understanding tick and tick-borne disease (TBD) epidemiology. There is evidence that ticks differentially express specific sets of genes when stimulated to start feeding. This study was initiated to investigate if ticks such as Ixodes scapularis and Amblyomma americanum that are adapted to feed on multiple hosts utilized the same sets of proteins to prepare for feeding. We exposed I. scapularis and A. americanum to feeding stimuli of different hosts (rabbit, human, and dog) by keeping unfed adult ticks enclosed in a perforated microfuge in close contact with host skin, but not allowing ticks to attach on host. Our data suggest that ticks of the same species differentially express tick saliva proteins (TSPs) when stimulated to start feeding on different hosts. SDS-PAGE and silver staining analysis revealed unique electrophoretic profiles in saliva of I. scapularis and A. americanum that were stimulated to feed on different hosts: rabbit, human, and dog. LC-MS/MS sequencing and pairwise analysis demonstrated that I. scapularis and A. americanum ticks expressed unique protein profiles in their saliva when stimulated to start feeding on different hosts: rabbit, dog, or human. Specifically, our data revealed TSPs that were unique to each treatment and those that were shared between treatments. Overall, we identified a total of 276 and 340 non-redundant I. scapularis and A. americanum TSPs, which we have classified into 28 functional classes including: secreted conserved proteins (unknown functions), proteinase inhibitors, lipocalins, extracellularmatrix/cell adhesion, heme/ironmetabolism, signal transduction and immunity-related proteins being the most predominant in saliva of unfed ticks. With exception of research on vaccines against Rhipicephalus microplus, which its natural host, cattle, research on vaccine against other ticks relies feeding ticks on laboratory animals. Data here suggest that relying on lab animal tick feeding data to select target antigens could result in prioritizing irrelevant anti-tick vaccine targets that are expressed when ticks feed on laboratory animals. This study provides the platform that could be utilized to identify relevant target anti-tick vaccine antigens, and will facilitate early stage tick feeding research.
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spelling Tirloni, LucasKim, Tae KwonPinto, Antonio Frederico MichelYates III, John R.Vaz Junior, Itabajara da SilvaMulenga, Albert2018-03-14T02:38:18Z20172235-2988http://hdl.handle.net/10183/173336001056541Understanding the molecular basis of how ticks adapt to feed on different animal hosts is central to understanding tick and tick-borne disease (TBD) epidemiology. There is evidence that ticks differentially express specific sets of genes when stimulated to start feeding. This study was initiated to investigate if ticks such as Ixodes scapularis and Amblyomma americanum that are adapted to feed on multiple hosts utilized the same sets of proteins to prepare for feeding. We exposed I. scapularis and A. americanum to feeding stimuli of different hosts (rabbit, human, and dog) by keeping unfed adult ticks enclosed in a perforated microfuge in close contact with host skin, but not allowing ticks to attach on host. Our data suggest that ticks of the same species differentially express tick saliva proteins (TSPs) when stimulated to start feeding on different hosts. SDS-PAGE and silver staining analysis revealed unique electrophoretic profiles in saliva of I. scapularis and A. americanum that were stimulated to feed on different hosts: rabbit, human, and dog. LC-MS/MS sequencing and pairwise analysis demonstrated that I. scapularis and A. americanum ticks expressed unique protein profiles in their saliva when stimulated to start feeding on different hosts: rabbit, dog, or human. Specifically, our data revealed TSPs that were unique to each treatment and those that were shared between treatments. Overall, we identified a total of 276 and 340 non-redundant I. scapularis and A. americanum TSPs, which we have classified into 28 functional classes including: secreted conserved proteins (unknown functions), proteinase inhibitors, lipocalins, extracellularmatrix/cell adhesion, heme/ironmetabolism, signal transduction and immunity-related proteins being the most predominant in saliva of unfed ticks. With exception of research on vaccines against Rhipicephalus microplus, which its natural host, cattle, research on vaccine against other ticks relies feeding ticks on laboratory animals. Data here suggest that relying on lab animal tick feeding data to select target antigens could result in prioritizing irrelevant anti-tick vaccine targets that are expressed when ticks feed on laboratory animals. This study provides the platform that could be utilized to identify relevant target anti-tick vaccine antigens, and will facilitate early stage tick feeding research.application/pdfengFrontiers in cellular and infection microbiology. Lausanne. Vol. 7, Article 517, (Dec. 2017), 13 p.CarrapatoIxodes scapularisAmblyomma americanumGenética molecularSalivaProteômicaProteínasHospedeiroTickSalivaProteomicTick-host relationshipHost adaptationTick-host range adaptation : changes in protein profiles in unfed adult Ixodes scapularis and Amblyomma americanum saliva stimulated to feed on different hostsEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001056541.pdf001056541.pdfTexto completo (inglês)application/pdf4622569http://www.lume.ufrgs.br/bitstream/10183/173336/1/001056541.pdf584f0789890535a280fa4581c8c1b73bMD51TEXT001056541.pdf.txt001056541.pdf.txtExtracted Texttext/plain66756http://www.lume.ufrgs.br/bitstream/10183/173336/2/001056541.pdf.txtb9c4a57ddb3b7ff21ae67fbb0bc55aa5MD5210183/1733362018-03-15 02:31:54.777213oai:www.lume.ufrgs.br:10183/173336Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-03-15T05:31:54Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Tick-host range adaptation : changes in protein profiles in unfed adult Ixodes scapularis and Amblyomma americanum saliva stimulated to feed on different hosts
title Tick-host range adaptation : changes in protein profiles in unfed adult Ixodes scapularis and Amblyomma americanum saliva stimulated to feed on different hosts
spellingShingle Tick-host range adaptation : changes in protein profiles in unfed adult Ixodes scapularis and Amblyomma americanum saliva stimulated to feed on different hosts
Tirloni, Lucas
Carrapato
Ixodes scapularis
Amblyomma americanum
Genética molecular
Saliva
Proteômica
Proteínas
Hospedeiro
Tick
Saliva
Proteomic
Tick-host relationship
Host adaptation
title_short Tick-host range adaptation : changes in protein profiles in unfed adult Ixodes scapularis and Amblyomma americanum saliva stimulated to feed on different hosts
title_full Tick-host range adaptation : changes in protein profiles in unfed adult Ixodes scapularis and Amblyomma americanum saliva stimulated to feed on different hosts
title_fullStr Tick-host range adaptation : changes in protein profiles in unfed adult Ixodes scapularis and Amblyomma americanum saliva stimulated to feed on different hosts
title_full_unstemmed Tick-host range adaptation : changes in protein profiles in unfed adult Ixodes scapularis and Amblyomma americanum saliva stimulated to feed on different hosts
title_sort Tick-host range adaptation : changes in protein profiles in unfed adult Ixodes scapularis and Amblyomma americanum saliva stimulated to feed on different hosts
author Tirloni, Lucas
author_facet Tirloni, Lucas
Kim, Tae Kwon
Pinto, Antonio Frederico Michel
Yates III, John R.
Vaz Junior, Itabajara da Silva
Mulenga, Albert
author_role author
author2 Kim, Tae Kwon
Pinto, Antonio Frederico Michel
Yates III, John R.
Vaz Junior, Itabajara da Silva
Mulenga, Albert
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Tirloni, Lucas
Kim, Tae Kwon
Pinto, Antonio Frederico Michel
Yates III, John R.
Vaz Junior, Itabajara da Silva
Mulenga, Albert
dc.subject.por.fl_str_mv Carrapato
Ixodes scapularis
Amblyomma americanum
Genética molecular
Saliva
Proteômica
Proteínas
Hospedeiro
topic Carrapato
Ixodes scapularis
Amblyomma americanum
Genética molecular
Saliva
Proteômica
Proteínas
Hospedeiro
Tick
Saliva
Proteomic
Tick-host relationship
Host adaptation
dc.subject.eng.fl_str_mv Tick
Saliva
Proteomic
Tick-host relationship
Host adaptation
description Understanding the molecular basis of how ticks adapt to feed on different animal hosts is central to understanding tick and tick-borne disease (TBD) epidemiology. There is evidence that ticks differentially express specific sets of genes when stimulated to start feeding. This study was initiated to investigate if ticks such as Ixodes scapularis and Amblyomma americanum that are adapted to feed on multiple hosts utilized the same sets of proteins to prepare for feeding. We exposed I. scapularis and A. americanum to feeding stimuli of different hosts (rabbit, human, and dog) by keeping unfed adult ticks enclosed in a perforated microfuge in close contact with host skin, but not allowing ticks to attach on host. Our data suggest that ticks of the same species differentially express tick saliva proteins (TSPs) when stimulated to start feeding on different hosts. SDS-PAGE and silver staining analysis revealed unique electrophoretic profiles in saliva of I. scapularis and A. americanum that were stimulated to feed on different hosts: rabbit, human, and dog. LC-MS/MS sequencing and pairwise analysis demonstrated that I. scapularis and A. americanum ticks expressed unique protein profiles in their saliva when stimulated to start feeding on different hosts: rabbit, dog, or human. Specifically, our data revealed TSPs that were unique to each treatment and those that were shared between treatments. Overall, we identified a total of 276 and 340 non-redundant I. scapularis and A. americanum TSPs, which we have classified into 28 functional classes including: secreted conserved proteins (unknown functions), proteinase inhibitors, lipocalins, extracellularmatrix/cell adhesion, heme/ironmetabolism, signal transduction and immunity-related proteins being the most predominant in saliva of unfed ticks. With exception of research on vaccines against Rhipicephalus microplus, which its natural host, cattle, research on vaccine against other ticks relies feeding ticks on laboratory animals. Data here suggest that relying on lab animal tick feeding data to select target antigens could result in prioritizing irrelevant anti-tick vaccine targets that are expressed when ticks feed on laboratory animals. This study provides the platform that could be utilized to identify relevant target anti-tick vaccine antigens, and will facilitate early stage tick feeding research.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-03-14T02:38:18Z
dc.type.driver.fl_str_mv Estrangeiro
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/173336
dc.identifier.issn.pt_BR.fl_str_mv 2235-2988
dc.identifier.nrb.pt_BR.fl_str_mv 001056541
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dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Frontiers in cellular and infection microbiology. Lausanne. Vol. 7, Article 517, (Dec. 2017), 13 p.
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