Localization and function of Rhipicephalus (Boophilus) microplus vitellin-degrading cysteine endopeptidase

• Autor(a) principal: Seixas, Adriana
• Data de Publicação: 2010
• Outros Autores: Estrela, Andreia Bergamo, Ceolato, Juliana Casagrande, Pontes, Emerson Guedes, Lara, Flávio Alves, Gondim, Katia Calp, Termignoni, Carlos
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/27466
• topic: Rhipicephalus microplus; Carrapatos; Cisteína endopeptidases; Bioquímica; Rhipicephalus (Boophilus) microplus; Cysteine endopeptidase; Cysteine endopeptidase inhibitor; Haemolymph; Vitellin; VTDCE

The tick Rhipicephalus (Boophilus) microplus is an important parasite of cattle in many areas of the tropics. Characterization of molecules involved in mechanisms such as vitellogenesis and embryo development may contribute to a better understanding of this parasite’s physiology. The vitellin-degrading cysteine endopeptidase (VTDCE) is the most active enzyme involved in vitellin hydrolysis in R. microplus eggs. Here we show an association between VTDCE and vitellin in an additional site, apart from the active site. Our data also demonstrate cysteine endopeptidase activity in different tissues such as ovary, gut, fat body, salivary gland and female haemolymph, where it is controlled by a physiological inhibitor. In R. microplus female gut, VTDCE is localized in areas of protein synthesis and trafficking with the underlying haemolymph. VTDCE is also localized in the ovary basal region, in vesicle membranes of ovary pedicel cells and in oocyte cytosol. These results suggest that VTDCE plays a role in vitellin digestion during tick development.