High levels of catalase in SOD mutants of Saccharomyces cerevisae in high aeration conditions

Detalhes bibliográficos
Autor(a) principal: Brandão, Vanessa Duarte Martins
Data de Publicação: 2005
Outros Autores: Manfredini, Vanusa, Benfato, Mara da Silveira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/20160
Resumo: Saccharomyces cerevisiae mutants deficient in superoxide dismutase genes (sod1Δ, sod2Δ and sod1Δsod2Δ mutants) in a stationary phase of growth under high aeration conditions were subjected to H2O2 stress. All the mutants were sensitive after H2O2 treatment. Glutathione peroxidase levels were significantly lower in sod1Δ and sod2Δ single mutants than in the wild-type without treatment. After exposure to H2O2 concentrations, glutathione peroxidase levels were increased in sod1Δsod2Δ double mutants and the sod2Δ single mutant, while sod1Δ maintained lower gluthatione peroxidase activities. The sod2Δ mutant demonstrated a similar catalase activity to that of the wild-type without treatment, whilst decreased catalase activity was observed in conditions of low aeration. Untreated sod1Δsod2Δ double mutant cells presented a lower catalase activity. Catalase levels were higher under high aeration conditions than under microaerophilic conditions, including in sod1Δsod2Δ cells that contain less H2O2, since SOD catalyzes the cleavage of superoxide producing H2O2 and oxygen. We suggest that catalase is not essential for sod mutants under normal conditions, but plays an important role in the acquisition of tolerance to oxidative stress induced by high aeration.
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spelling Brandão, Vanessa Duarte MartinsManfredini, VanusaBenfato, Mara da Silveira2010-04-16T09:13:19Z20051517-8382http://hdl.handle.net/10183/20160000521604Saccharomyces cerevisiae mutants deficient in superoxide dismutase genes (sod1Δ, sod2Δ and sod1Δsod2Δ mutants) in a stationary phase of growth under high aeration conditions were subjected to H2O2 stress. All the mutants were sensitive after H2O2 treatment. Glutathione peroxidase levels were significantly lower in sod1Δ and sod2Δ single mutants than in the wild-type without treatment. After exposure to H2O2 concentrations, glutathione peroxidase levels were increased in sod1Δsod2Δ double mutants and the sod2Δ single mutant, while sod1Δ maintained lower gluthatione peroxidase activities. The sod2Δ mutant demonstrated a similar catalase activity to that of the wild-type without treatment, whilst decreased catalase activity was observed in conditions of low aeration. Untreated sod1Δsod2Δ double mutant cells presented a lower catalase activity. Catalase levels were higher under high aeration conditions than under microaerophilic conditions, including in sod1Δsod2Δ cells that contain less H2O2, since SOD catalyzes the cleavage of superoxide producing H2O2 and oxygen. We suggest that catalase is not essential for sod mutants under normal conditions, but plays an important role in the acquisition of tolerance to oxidative stress induced by high aeration.application/pdfengBrazilian journal of microbiology. São Paulo, SP. Vol. 36, n. 4, (out./dez. 2005), p. 347-351CatalaseSaccharomyces cerevisiaeSuperóxido dismutaseAeraçãoPeróxido de hidrogênioCatalaseSuperoxide dismutaseHigh aerationHydrogen peroxideS. cerevisiaeHigh levels of catalase in SOD mutants of Saccharomyces cerevisae in high aeration conditionsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000521604.pdf000521604.pdfTexto completo (inglês)application/pdf136937http://www.lume.ufrgs.br/bitstream/10183/20160/1/000521604.pdf491e56fa367afdb94b982bfa7913a64aMD51TEXT000521604.pdf.txt000521604.pdf.txtExtracted Texttext/plain19356http://www.lume.ufrgs.br/bitstream/10183/20160/2/000521604.pdf.txt810756243f91cac4423072537c1c8ccaMD52THUMBNAIL000521604.pdf.jpg000521604.pdf.jpgGenerated Thumbnailimage/jpeg1766http://www.lume.ufrgs.br/bitstream/10183/20160/3/000521604.pdf.jpg61b7cfbd070c04602762ac3e24915a5bMD5310183/201602018-10-08 09:13:02.831oai:www.lume.ufrgs.br:10183/20160Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-08T12:13:02Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv High levels of catalase in SOD mutants of Saccharomyces cerevisae in high aeration conditions
title High levels of catalase in SOD mutants of Saccharomyces cerevisae in high aeration conditions
spellingShingle High levels of catalase in SOD mutants of Saccharomyces cerevisae in high aeration conditions
Brandão, Vanessa Duarte Martins
Catalase
Saccharomyces cerevisiae
Superóxido dismutase
Aeração
Peróxido de hidrogênio
Catalase
Superoxide dismutase
High aeration
Hydrogen peroxide
S. cerevisiae
title_short High levels of catalase in SOD mutants of Saccharomyces cerevisae in high aeration conditions
title_full High levels of catalase in SOD mutants of Saccharomyces cerevisae in high aeration conditions
title_fullStr High levels of catalase in SOD mutants of Saccharomyces cerevisae in high aeration conditions
title_full_unstemmed High levels of catalase in SOD mutants of Saccharomyces cerevisae in high aeration conditions
title_sort High levels of catalase in SOD mutants of Saccharomyces cerevisae in high aeration conditions
author Brandão, Vanessa Duarte Martins
author_facet Brandão, Vanessa Duarte Martins
Manfredini, Vanusa
Benfato, Mara da Silveira
author_role author
author2 Manfredini, Vanusa
Benfato, Mara da Silveira
author2_role author
author
dc.contributor.author.fl_str_mv Brandão, Vanessa Duarte Martins
Manfredini, Vanusa
Benfato, Mara da Silveira
dc.subject.por.fl_str_mv Catalase
Saccharomyces cerevisiae
Superóxido dismutase
Aeração
Peróxido de hidrogênio
topic Catalase
Saccharomyces cerevisiae
Superóxido dismutase
Aeração
Peróxido de hidrogênio
Catalase
Superoxide dismutase
High aeration
Hydrogen peroxide
S. cerevisiae
dc.subject.eng.fl_str_mv Catalase
Superoxide dismutase
High aeration
Hydrogen peroxide
S. cerevisiae
description Saccharomyces cerevisiae mutants deficient in superoxide dismutase genes (sod1Δ, sod2Δ and sod1Δsod2Δ mutants) in a stationary phase of growth under high aeration conditions were subjected to H2O2 stress. All the mutants were sensitive after H2O2 treatment. Glutathione peroxidase levels were significantly lower in sod1Δ and sod2Δ single mutants than in the wild-type without treatment. After exposure to H2O2 concentrations, glutathione peroxidase levels were increased in sod1Δsod2Δ double mutants and the sod2Δ single mutant, while sod1Δ maintained lower gluthatione peroxidase activities. The sod2Δ mutant demonstrated a similar catalase activity to that of the wild-type without treatment, whilst decreased catalase activity was observed in conditions of low aeration. Untreated sod1Δsod2Δ double mutant cells presented a lower catalase activity. Catalase levels were higher under high aeration conditions than under microaerophilic conditions, including in sod1Δsod2Δ cells that contain less H2O2, since SOD catalyzes the cleavage of superoxide producing H2O2 and oxygen. We suggest that catalase is not essential for sod mutants under normal conditions, but plays an important role in the acquisition of tolerance to oxidative stress induced by high aeration.
publishDate 2005
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dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Brazilian journal of microbiology. São Paulo, SP. Vol. 36, n. 4, (out./dez. 2005), p. 347-351
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