Coxiella endosymbiont of Rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/241212 |
Resumo: | In the past decade, metagenomics studies exploring tick microbiota have revealed widespread interactions between bacteria and arthropods, including symbiotic interactions. Functional studies showed that obligate endosymbionts contribute to tick biology, affecting reproductive fitness and molting. Understanding the molecular basis of the interaction between ticks and their mutualist endosymbionts may help to develop control methods based on microbiome manipulation. Previously, we showed that Rhipicephalus microplus larvae with reduced levels of Coxiella endosymbiont of R. microplus (CERM) were arrested at the metanymph life stage (partially engorged nymph) and did not molt into adults. In this study, we performed a transcriptomic differential analysis of the R. microplus metanymph in the presence and absence of its mutualist endosymbiont. The lack of CERM resulted in an altered expression profile of transcripts from several functional categories. Gene products such as DA-P36, protease inhibitors, metalloproteases, and evasins, which are involved in blood feeding capacity, were underexpressed in CERM-free metanymphs. Disregulation in genes related to extracellular matrix remodeling was also observed in the absence of the symbiont. Taken together, the observed alterations in gene expression may explain the blockage of development at the metanymph stage and reveal a novel physiological aspect of the symbiont-tick-vertebrate host interaction. |
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Guizzo, Melina GarciaTirloni, LucasGonzalez, Sergio AlbertoFarber, Marisa DianaBraz, Glória Regina CardosoParizi, Luis FernandoSilva, Lucas André Dedavid eVaz Junior, Itabajara da SilvaOliveira, Pedro Lagerblad de2022-06-25T05:08:20Z20221664-302xhttp://hdl.handle.net/10183/241212001140670In the past decade, metagenomics studies exploring tick microbiota have revealed widespread interactions between bacteria and arthropods, including symbiotic interactions. Functional studies showed that obligate endosymbionts contribute to tick biology, affecting reproductive fitness and molting. Understanding the molecular basis of the interaction between ticks and their mutualist endosymbionts may help to develop control methods based on microbiome manipulation. Previously, we showed that Rhipicephalus microplus larvae with reduced levels of Coxiella endosymbiont of R. microplus (CERM) were arrested at the metanymph life stage (partially engorged nymph) and did not molt into adults. In this study, we performed a transcriptomic differential analysis of the R. microplus metanymph in the presence and absence of its mutualist endosymbiont. The lack of CERM resulted in an altered expression profile of transcripts from several functional categories. Gene products such as DA-P36, protease inhibitors, metalloproteases, and evasins, which are involved in blood feeding capacity, were underexpressed in CERM-free metanymphs. Disregulation in genes related to extracellular matrix remodeling was also observed in the absence of the symbiont. Taken together, the observed alterations in gene expression may explain the blockage of development at the metanymph stage and reveal a novel physiological aspect of the symbiont-tick-vertebrate host interaction.application/pdfengFrontiers in Microbiology. Lausanne. Vol. 13 (May 2022), 868575, 13 p.SimbioseCoxiellaRhipicephalus microplusAlimentação hematófagaCrescimento e desenvolvimentoTranscriptomaSymbiontMicrobiomeTickTranscriptomeCoxiella endosymbiont of Rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacityEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001140670.pdf.txt001140670.pdf.txtExtracted Texttext/plain75015http://www.lume.ufrgs.br/bitstream/10183/241212/2/001140670.pdf.txt2b70c84ae08b6f5d68bd27e9d29d79a3MD52ORIGINAL001140670.pdfTexto completo (inglês)application/pdf1594645http://www.lume.ufrgs.br/bitstream/10183/241212/1/001140670.pdf7e8b9071da0a1c5ac1db6f781bc2db8aMD5110183/2412122022-06-26 04:47:48.922802oai:www.lume.ufrgs.br:10183/241212Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2022-06-26T07:47:48Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Coxiella endosymbiont of Rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity |
title |
Coxiella endosymbiont of Rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity |
spellingShingle |
Coxiella endosymbiont of Rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity Guizzo, Melina Garcia Simbiose Coxiella Rhipicephalus microplus Alimentação hematófaga Crescimento e desenvolvimento Transcriptoma Symbiont Microbiome Tick Transcriptome |
title_short |
Coxiella endosymbiont of Rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity |
title_full |
Coxiella endosymbiont of Rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity |
title_fullStr |
Coxiella endosymbiont of Rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity |
title_full_unstemmed |
Coxiella endosymbiont of Rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity |
title_sort |
Coxiella endosymbiont of Rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity |
author |
Guizzo, Melina Garcia |
author_facet |
Guizzo, Melina Garcia Tirloni, Lucas Gonzalez, Sergio Alberto Farber, Marisa Diana Braz, Glória Regina Cardoso Parizi, Luis Fernando Silva, Lucas André Dedavid e Vaz Junior, Itabajara da Silva Oliveira, Pedro Lagerblad de |
author_role |
author |
author2 |
Tirloni, Lucas Gonzalez, Sergio Alberto Farber, Marisa Diana Braz, Glória Regina Cardoso Parizi, Luis Fernando Silva, Lucas André Dedavid e Vaz Junior, Itabajara da Silva Oliveira, Pedro Lagerblad de |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Guizzo, Melina Garcia Tirloni, Lucas Gonzalez, Sergio Alberto Farber, Marisa Diana Braz, Glória Regina Cardoso Parizi, Luis Fernando Silva, Lucas André Dedavid e Vaz Junior, Itabajara da Silva Oliveira, Pedro Lagerblad de |
dc.subject.por.fl_str_mv |
Simbiose Coxiella Rhipicephalus microplus Alimentação hematófaga Crescimento e desenvolvimento Transcriptoma |
topic |
Simbiose Coxiella Rhipicephalus microplus Alimentação hematófaga Crescimento e desenvolvimento Transcriptoma Symbiont Microbiome Tick Transcriptome |
dc.subject.eng.fl_str_mv |
Symbiont Microbiome Tick Transcriptome |
description |
In the past decade, metagenomics studies exploring tick microbiota have revealed widespread interactions between bacteria and arthropods, including symbiotic interactions. Functional studies showed that obligate endosymbionts contribute to tick biology, affecting reproductive fitness and molting. Understanding the molecular basis of the interaction between ticks and their mutualist endosymbionts may help to develop control methods based on microbiome manipulation. Previously, we showed that Rhipicephalus microplus larvae with reduced levels of Coxiella endosymbiont of R. microplus (CERM) were arrested at the metanymph life stage (partially engorged nymph) and did not molt into adults. In this study, we performed a transcriptomic differential analysis of the R. microplus metanymph in the presence and absence of its mutualist endosymbiont. The lack of CERM resulted in an altered expression profile of transcripts from several functional categories. Gene products such as DA-P36, protease inhibitors, metalloproteases, and evasins, which are involved in blood feeding capacity, were underexpressed in CERM-free metanymphs. Disregulation in genes related to extracellular matrix remodeling was also observed in the absence of the symbiont. Taken together, the observed alterations in gene expression may explain the blockage of development at the metanymph stage and reveal a novel physiological aspect of the symbiont-tick-vertebrate host interaction. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-06-25T05:08:20Z |
dc.date.issued.fl_str_mv |
2022 |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/241212 |
dc.identifier.issn.pt_BR.fl_str_mv |
1664-302x |
dc.identifier.nrb.pt_BR.fl_str_mv |
001140670 |
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1664-302x 001140670 |
url |
http://hdl.handle.net/10183/241212 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Frontiers in Microbiology. Lausanne. Vol. 13 (May 2022), 868575, 13 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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