Phenotypic and genotypic characterization of Salmonella enteritidis isolates
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/20537 |
Resumo: | In order to study the epidemiology of Salmonella Enteritidis outbreaks and determine the source of contamination so that a recurrence can be avoided, detailed characterization is necessary. Thus, the purpose of this study was to verify whether rep-PCR was able to discriminate among Salmonella Enteritidis isolates. Phage typing, detection of virulence genes and antimicrobial resistance testing were also associated to rep- PCR results. One hundred and two S. Enteritidis isolates from broiler carcasses, food, human, pigs, poultryrelated samples, and nine isolates from other countries were genotypically typed by REP-PCR, ERIC-PCR and BOX-PCR, collectively called rep-PCR. Phage typing, detection of virulence genes and antimicrobial resistance testing were also performed. Only three fingerprinting profiles were obtained with each rep-PCR method, with the majority of isolates belonging to the same profile. No relationship was observed between genotypic profile and year, place of isolation or source of infection. However, the less frequent rep-PCR profiles showed single antimicrobial resistance patterns. Although few strains isolated from swine were analyzed, different antimicrobial resistance patterns were observed. Furthermore, phage type 4 was not found in swine isolates. rep-PCR showed a lower discriminatory power as compared with antimicrobial resistance and phage typing, but the combination of genotypic and phenotypic methods was more discriminatory than any method alone, resulting in 48 different types. |
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Oliveira, Sílvia Dias deBessa, Marjô CadóSantos, Luciana Ruschel dosCardoso, Marisa Ribeiro de ItapemaBrandelli, AdrianoCanal, Cláudio Wageck2010-04-16T09:15:21Z20071517-8382http://hdl.handle.net/10183/20537000630385In order to study the epidemiology of Salmonella Enteritidis outbreaks and determine the source of contamination so that a recurrence can be avoided, detailed characterization is necessary. Thus, the purpose of this study was to verify whether rep-PCR was able to discriminate among Salmonella Enteritidis isolates. Phage typing, detection of virulence genes and antimicrobial resistance testing were also associated to rep- PCR results. One hundred and two S. Enteritidis isolates from broiler carcasses, food, human, pigs, poultryrelated samples, and nine isolates from other countries were genotypically typed by REP-PCR, ERIC-PCR and BOX-PCR, collectively called rep-PCR. Phage typing, detection of virulence genes and antimicrobial resistance testing were also performed. Only three fingerprinting profiles were obtained with each rep-PCR method, with the majority of isolates belonging to the same profile. No relationship was observed between genotypic profile and year, place of isolation or source of infection. However, the less frequent rep-PCR profiles showed single antimicrobial resistance patterns. Although few strains isolated from swine were analyzed, different antimicrobial resistance patterns were observed. Furthermore, phage type 4 was not found in swine isolates. rep-PCR showed a lower discriminatory power as compared with antimicrobial resistance and phage typing, but the combination of genotypic and phenotypic methods was more discriminatory than any method alone, resulting in 48 different types.application/pdfengBrazilian journal of microbiology. São Paulo. Vol. 38, n. 4 (oct./dec. 2007), p. 720-728Salmonella enteritidisSalmonella EnteritidisRep-PCRPhage typingAntimicrobial resistanceVirulence genesPhenotypic and genotypic characterization of Salmonella enteritidis isolatesinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000630385.pdf000630385.pdfTexto completo (inglês)application/pdf209793http://www.lume.ufrgs.br/bitstream/10183/20537/1/000630385.pdfc7a8dde72ea2dcac38d485144da0a046MD51TEXT000630385.pdf.txt000630385.pdf.txtExtracted Texttext/plain40648http://www.lume.ufrgs.br/bitstream/10183/20537/2/000630385.pdf.txtf43750490f5e7547843effb16c77f715MD52THUMBNAIL000630385.pdf.jpg000630385.pdf.jpgGenerated Thumbnailimage/jpeg1763http://www.lume.ufrgs.br/bitstream/10183/20537/3/000630385.pdf.jpgdfeafb9aeb702c22863b807a17967f16MD5310183/205372018-10-08 09:14:29.021oai:www.lume.ufrgs.br:10183/20537Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-08T12:14:29Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Phenotypic and genotypic characterization of Salmonella enteritidis isolates |
title |
Phenotypic and genotypic characterization of Salmonella enteritidis isolates |
spellingShingle |
Phenotypic and genotypic characterization of Salmonella enteritidis isolates Oliveira, Sílvia Dias de Salmonella enteritidis Salmonella Enteritidis Rep-PCR Phage typing Antimicrobial resistance Virulence genes |
title_short |
Phenotypic and genotypic characterization of Salmonella enteritidis isolates |
title_full |
Phenotypic and genotypic characterization of Salmonella enteritidis isolates |
title_fullStr |
Phenotypic and genotypic characterization of Salmonella enteritidis isolates |
title_full_unstemmed |
Phenotypic and genotypic characterization of Salmonella enteritidis isolates |
title_sort |
Phenotypic and genotypic characterization of Salmonella enteritidis isolates |
author |
Oliveira, Sílvia Dias de |
author_facet |
Oliveira, Sílvia Dias de Bessa, Marjô Cadó Santos, Luciana Ruschel dos Cardoso, Marisa Ribeiro de Itapema Brandelli, Adriano Canal, Cláudio Wageck |
author_role |
author |
author2 |
Bessa, Marjô Cadó Santos, Luciana Ruschel dos Cardoso, Marisa Ribeiro de Itapema Brandelli, Adriano Canal, Cláudio Wageck |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Sílvia Dias de Bessa, Marjô Cadó Santos, Luciana Ruschel dos Cardoso, Marisa Ribeiro de Itapema Brandelli, Adriano Canal, Cláudio Wageck |
dc.subject.por.fl_str_mv |
Salmonella enteritidis |
topic |
Salmonella enteritidis Salmonella Enteritidis Rep-PCR Phage typing Antimicrobial resistance Virulence genes |
dc.subject.eng.fl_str_mv |
Salmonella Enteritidis Rep-PCR Phage typing Antimicrobial resistance Virulence genes |
description |
In order to study the epidemiology of Salmonella Enteritidis outbreaks and determine the source of contamination so that a recurrence can be avoided, detailed characterization is necessary. Thus, the purpose of this study was to verify whether rep-PCR was able to discriminate among Salmonella Enteritidis isolates. Phage typing, detection of virulence genes and antimicrobial resistance testing were also associated to rep- PCR results. One hundred and two S. Enteritidis isolates from broiler carcasses, food, human, pigs, poultryrelated samples, and nine isolates from other countries were genotypically typed by REP-PCR, ERIC-PCR and BOX-PCR, collectively called rep-PCR. Phage typing, detection of virulence genes and antimicrobial resistance testing were also performed. Only three fingerprinting profiles were obtained with each rep-PCR method, with the majority of isolates belonging to the same profile. No relationship was observed between genotypic profile and year, place of isolation or source of infection. However, the less frequent rep-PCR profiles showed single antimicrobial resistance patterns. Although few strains isolated from swine were analyzed, different antimicrobial resistance patterns were observed. Furthermore, phage type 4 was not found in swine isolates. rep-PCR showed a lower discriminatory power as compared with antimicrobial resistance and phage typing, but the combination of genotypic and phenotypic methods was more discriminatory than any method alone, resulting in 48 different types. |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007 |
dc.date.accessioned.fl_str_mv |
2010-04-16T09:15:21Z |
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info:eu-repo/semantics/article info:eu-repo/semantics/other |
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http://hdl.handle.net/10183/20537 |
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1517-8382 |
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000630385 |
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http://hdl.handle.net/10183/20537 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of microbiology. São Paulo. Vol. 38, n. 4 (oct./dec. 2007), p. 720-728 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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