Production of polyhydroxyalkanoates by Bacillus megaterium : prospecting on rice hull and residual glycerol potential

• Autor(a) principal: Schmidt, Vanessa Kristine de Oliveira
• Data de Publicação: 2022
• Outros Autores: Santos, Evelise Fonseca dos, Oliveira, Débora de, Ayub, Marco Antônio Záchia, Cesca, Karina, Dall Cortivo, Paulo Roberto, Andrade, Cristiano José de, Hickert, Lilian Raquel
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/257879
• topic: Biopolímero; Glicerol residual; Casca de arroz; Hidrólise; PHA; Biopolymers; Residual glycerol; Rice hull hydrolysate

The production of polyhydroxyalkanoates (PHAs) by Bacillus megaterium using industrial residues, crude glycerol from biodiesel synthesis and rice hull hydrolysate (RHH), as low-cost carbon sources was investigated. The experiments were conducted by shaking flasks at 30 C and 180 rpm up to 72 h. The extraction of PHA was carried out using sodium hypochlorite to make its recovery more environmentally friendly by avoiding organic solvents (chloroform). The yields of PHA varied depending on the extraction method. A total of 33.3% (w w1) (mixing chloroform: sodium hypochlorite) and 52.5% (w w1) (sodium hypochlorite only) were obtained using glycerol and glucose as a carbon source, respectively. Preliminary experiments using RHH as a carbon source Indicated a yield of PHA of 11% (w w1) (chloroform). The PHA produced had thermal properties, such as transition temperature, similar to the commercial polyhydroxybutyrate (PHB).