A Model system to study antimicrobial strategies in endodontic biofilms

Detalhes bibliográficos
Autor(a) principal: Estrela, Carlos
Data de Publicação: 2009
Outros Autores: Sydney, Gilson Blitzkow, Figueiredo, Jose Antonio Poli de, Estrela, Cyntia Rodrigues de Araújo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/61173
Resumo: The purpose of this work was to develop a model system to study antimicrobial strategies in endodontic biofilms. Enterococcus faecalis suspension was colonized in 10 human root canals. Five milliliters of Brain Heart Infusion (BHI) were mixed with 5 mL of the bacterial inoculums (E. faecalis) and inoculated with sufficient volume to fill the root canal during 60 days. This procedure was repeated every 72 h, always using 24-h pure culture prepared and adjusted to No. 1 MacFarland turbidity standard. Biofilm formation was analyzed by scanning electron microscopy (SEM). E. faecalis consistently adhered to collagen structure, colonized dentin surface, progressed towards the dentinal tubules and formed a biofilm. The proposed biofilm model seems to be viable for studies on antimicrobial strategies, and allows for a satisfactory colonization time of selected bacterial species with virulence and adherence properties.
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spelling Estrela, CarlosSydney, Gilson BlitzkowFigueiredo, Jose Antonio Poli deEstrela, Cyntia Rodrigues de Araújo2012-11-21T01:50:42Z20091678-7757http://hdl.handle.net/10183/61173000707261The purpose of this work was to develop a model system to study antimicrobial strategies in endodontic biofilms. Enterococcus faecalis suspension was colonized in 10 human root canals. Five milliliters of Brain Heart Infusion (BHI) were mixed with 5 mL of the bacterial inoculums (E. faecalis) and inoculated with sufficient volume to fill the root canal during 60 days. This procedure was repeated every 72 h, always using 24-h pure culture prepared and adjusted to No. 1 MacFarland turbidity standard. Biofilm formation was analyzed by scanning electron microscopy (SEM). E. faecalis consistently adhered to collagen structure, colonized dentin surface, progressed towards the dentinal tubules and formed a biofilm. The proposed biofilm model seems to be viable for studies on antimicrobial strategies, and allows for a satisfactory colonization time of selected bacterial species with virulence and adherence properties.application/pdfengJournal of applied oral science. Bauru. Vol. 17, no. 2 (Mar./Apr. 2009), p. 87-91BiofilmesAntimicrobianosEnterococcus faecalisCanais radicularesEndodontiaBiofilmEndodontic biofilmEnterococcus faecalisIntracanal medicamentsAntimicrobial strategiesA Model system to study antimicrobial strategies in endodontic biofilmsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000707261.pdf000707261.pdfTexto completo (inglês)application/pdf584100http://www.lume.ufrgs.br/bitstream/10183/61173/1/000707261.pdf312c83bf67c650ab821b212492cc5cafMD51TEXT000707261.pdf.txt000707261.pdf.txtExtracted Texttext/plain25480http://www.lume.ufrgs.br/bitstream/10183/61173/2/000707261.pdf.txt550dc39c046ff0d45d9d217ff44acb98MD52THUMBNAIL000707261.pdf.jpg000707261.pdf.jpgGenerated Thumbnailimage/jpeg2008http://www.lume.ufrgs.br/bitstream/10183/61173/3/000707261.pdf.jpgdd770f11fad5055f6df3b45f97a194e0MD5310183/611732023-08-11 03:53:02.980854oai:www.lume.ufrgs.br:10183/61173Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-08-11T06:53:02Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv A Model system to study antimicrobial strategies in endodontic biofilms
title A Model system to study antimicrobial strategies in endodontic biofilms
spellingShingle A Model system to study antimicrobial strategies in endodontic biofilms
Estrela, Carlos
Biofilmes
Antimicrobianos
Enterococcus faecalis
Canais radiculares
Endodontia
Biofilm
Endodontic biofilm
Enterococcus faecalis
Intracanal medicaments
Antimicrobial strategies
title_short A Model system to study antimicrobial strategies in endodontic biofilms
title_full A Model system to study antimicrobial strategies in endodontic biofilms
title_fullStr A Model system to study antimicrobial strategies in endodontic biofilms
title_full_unstemmed A Model system to study antimicrobial strategies in endodontic biofilms
title_sort A Model system to study antimicrobial strategies in endodontic biofilms
author Estrela, Carlos
author_facet Estrela, Carlos
Sydney, Gilson Blitzkow
Figueiredo, Jose Antonio Poli de
Estrela, Cyntia Rodrigues de Araújo
author_role author
author2 Sydney, Gilson Blitzkow
Figueiredo, Jose Antonio Poli de
Estrela, Cyntia Rodrigues de Araújo
author2_role author
author
author
dc.contributor.author.fl_str_mv Estrela, Carlos
Sydney, Gilson Blitzkow
Figueiredo, Jose Antonio Poli de
Estrela, Cyntia Rodrigues de Araújo
dc.subject.por.fl_str_mv Biofilmes
Antimicrobianos
Enterococcus faecalis
Canais radiculares
Endodontia
topic Biofilmes
Antimicrobianos
Enterococcus faecalis
Canais radiculares
Endodontia
Biofilm
Endodontic biofilm
Enterococcus faecalis
Intracanal medicaments
Antimicrobial strategies
dc.subject.eng.fl_str_mv Biofilm
Endodontic biofilm
Enterococcus faecalis
Intracanal medicaments
Antimicrobial strategies
description The purpose of this work was to develop a model system to study antimicrobial strategies in endodontic biofilms. Enterococcus faecalis suspension was colonized in 10 human root canals. Five milliliters of Brain Heart Infusion (BHI) were mixed with 5 mL of the bacterial inoculums (E. faecalis) and inoculated with sufficient volume to fill the root canal during 60 days. This procedure was repeated every 72 h, always using 24-h pure culture prepared and adjusted to No. 1 MacFarland turbidity standard. Biofilm formation was analyzed by scanning electron microscopy (SEM). E. faecalis consistently adhered to collagen structure, colonized dentin surface, progressed towards the dentinal tubules and formed a biofilm. The proposed biofilm model seems to be viable for studies on antimicrobial strategies, and allows for a satisfactory colonization time of selected bacterial species with virulence and adherence properties.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2012-11-21T01:50:42Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/61173
dc.identifier.issn.pt_BR.fl_str_mv 1678-7757
dc.identifier.nrb.pt_BR.fl_str_mv 000707261
identifier_str_mv 1678-7757
000707261
url http://hdl.handle.net/10183/61173
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Journal of applied oral science. Bauru. Vol. 17, no. 2 (Mar./Apr. 2009), p. 87-91
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRGS
instname:Universidade Federal do Rio Grande do Sul (UFRGS)
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institution UFRGS
reponame_str Repositório Institucional da UFRGS
collection Repositório Institucional da UFRGS
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