Associação entre fatores de virulência e resistência antimicrobiana de Escherichia coli enterotoxigênicas isoladas de leitões com diarreia no Brasil
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/223833 |
Resumo: | Background: Enterotoxigenic Escherichia coli (ETEC) is the etiologic agent of post weaning colibacillosis, one of the most important diseases in pig farming. The pathogenesis of the disease is associated with two virulence factors (VF), fimbriae and enterotoxins. In veterinary medicine, the use of antibiotics can lead to the selection of resistant bacteria. The association of VF and antibiotic resistance is an important mechanism for bacterial survival under adverse conditions. This study aimed to determine the VF and antimicrobial susceptibility of ETEC isolates from piglets with diarrhea and analyze the association between these factors. Materials, Methods & Results: A total of 185 rectal swabs were collected from weaned piglets in Brazilian farms of the states of Rio Grande do Sul, Santa Catarina, Paraná, Minas Gerais and Goiás. The isolation of ETEC was carried out on blood and MacConkey Agar and characterization by biochemical tests and detection by PCR of fimbrial genes F4, F45, F6, F18 and F41, and toxins genes LT, STa, STb and STx2e. Antimicrobial susceptibility profiles were determined by Agar diffusion test for amoxicillin, ampicillin, ceftiofur, ciprofloxacin, colistin, doxycycline, enrofloxacin, florfenicol, gentamicin, neomycin, norfloxacin, oxytetracycline, streptomycin, tetracycline, lincomycin + spectinomycin and sulfamethoxazole + trimethoprim. The association between VF and antimicrobials resistance results was determined by Chi-square and Fisher test (P ≤ 0.05). A total of 376 isolates were analyzed. The frequencies of fimbriae and toxins amplified were: F4 (31.6%), F18 (18.9%), F5 (4.2%) and toxins STa (43.1%), STb (24.7%), LT (21.8%) and STx2e (5.3%). Antibiotic resistance was higher to tetracycline (96.3%), florfenicol (95.2%), oxytetracycline (93.62%) and doxycycline (90.7%). Lowest levels of resistance were to ceftiofur (2.1%), colistin (9.8%), lincomycin + spectinomicin (15.4%) and neomycin (23.1%). The association of VF and resistance was significant for fimbriae F4 and streptomycin and sulfamethoxazole + trimethoprim; F5 and enrofloxacin, ciprofloxacin and norfloxacin; F18 and amoxicillin, ampicillin, doxycycline, enrofloxacin, norfloxacin, ciprofloxacin and sulfamethoxazole + trimethoprim. Among the toxins LT and tetracycline, streptomycin and florfenicol; STa and amoxicillin, ampicillin, oxytetracycline, enrofloxacin and sulfamethoxazole + trimethoprim; STb and colistin and streptomycin. Discussion: Resistance to all antimicrobials was observed, with higher levels for tetracyclines and lower to ceftiofur and lincomycin + spectinomycin. Several studies have reported ETEC virotype variation which can be influenced by management differences in pig farms. The presence of VF and acquisition of antimicrobial resistance mechanisms might be related to evolutionary factors, infection pressure and intensive use of antibiotics. E. coli is considered one of the most versatile bacterial species and its diversity in adaptability is due to its genomic plasticity, influencing the capacity to colonize numerous host species. This is possible by mechanisms such as gain or loss of genes through lateral transfer of plasmids, transposons and integrons, which vary regarding the environment to which they are exposed. More studies are needed to correlate genetically the interaction of antimicrobial resistance and virulence genes to elucidate if the virulence expression is affected by chromosomal mutations that lead to specific resistance or/ and both determinants are inserted into the same mobile genetic element, such as a conjugative plasmid. |
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Sato, José Paulo HirojiTakeuti, Karine LudwigDaniel, Amanda Gabrielle de SouzaKoerich, Priscilla K.V.Bernardi, Mari LourdesBarcellos, David Emilio Santos Neves de2021-07-14T04:31:01Z20151678-0345http://hdl.handle.net/10183/223833000985282Background: Enterotoxigenic Escherichia coli (ETEC) is the etiologic agent of post weaning colibacillosis, one of the most important diseases in pig farming. The pathogenesis of the disease is associated with two virulence factors (VF), fimbriae and enterotoxins. In veterinary medicine, the use of antibiotics can lead to the selection of resistant bacteria. The association of VF and antibiotic resistance is an important mechanism for bacterial survival under adverse conditions. This study aimed to determine the VF and antimicrobial susceptibility of ETEC isolates from piglets with diarrhea and analyze the association between these factors. Materials, Methods & Results: A total of 185 rectal swabs were collected from weaned piglets in Brazilian farms of the states of Rio Grande do Sul, Santa Catarina, Paraná, Minas Gerais and Goiás. The isolation of ETEC was carried out on blood and MacConkey Agar and characterization by biochemical tests and detection by PCR of fimbrial genes F4, F45, F6, F18 and F41, and toxins genes LT, STa, STb and STx2e. Antimicrobial susceptibility profiles were determined by Agar diffusion test for amoxicillin, ampicillin, ceftiofur, ciprofloxacin, colistin, doxycycline, enrofloxacin, florfenicol, gentamicin, neomycin, norfloxacin, oxytetracycline, streptomycin, tetracycline, lincomycin + spectinomycin and sulfamethoxazole + trimethoprim. The association between VF and antimicrobials resistance results was determined by Chi-square and Fisher test (P ≤ 0.05). A total of 376 isolates were analyzed. The frequencies of fimbriae and toxins amplified were: F4 (31.6%), F18 (18.9%), F5 (4.2%) and toxins STa (43.1%), STb (24.7%), LT (21.8%) and STx2e (5.3%). Antibiotic resistance was higher to tetracycline (96.3%), florfenicol (95.2%), oxytetracycline (93.62%) and doxycycline (90.7%). Lowest levels of resistance were to ceftiofur (2.1%), colistin (9.8%), lincomycin + spectinomicin (15.4%) and neomycin (23.1%). The association of VF and resistance was significant for fimbriae F4 and streptomycin and sulfamethoxazole + trimethoprim; F5 and enrofloxacin, ciprofloxacin and norfloxacin; F18 and amoxicillin, ampicillin, doxycycline, enrofloxacin, norfloxacin, ciprofloxacin and sulfamethoxazole + trimethoprim. Among the toxins LT and tetracycline, streptomycin and florfenicol; STa and amoxicillin, ampicillin, oxytetracycline, enrofloxacin and sulfamethoxazole + trimethoprim; STb and colistin and streptomycin. Discussion: Resistance to all antimicrobials was observed, with higher levels for tetracyclines and lower to ceftiofur and lincomycin + spectinomycin. Several studies have reported ETEC virotype variation which can be influenced by management differences in pig farms. The presence of VF and acquisition of antimicrobial resistance mechanisms might be related to evolutionary factors, infection pressure and intensive use of antibiotics. E. coli is considered one of the most versatile bacterial species and its diversity in adaptability is due to its genomic plasticity, influencing the capacity to colonize numerous host species. This is possible by mechanisms such as gain or loss of genes through lateral transfer of plasmids, transposons and integrons, which vary regarding the environment to which they are exposed. More studies are needed to correlate genetically the interaction of antimicrobial resistance and virulence genes to elucidate if the virulence expression is affected by chromosomal mutations that lead to specific resistance or/ and both determinants are inserted into the same mobile genetic element, such as a conjugative plasmid.application/pdfporActa scientiae veterinariae. Porto Alegre, RS. Vol. 43, (2015), [7 p.], Pub. 1329SuínoDiarréiaEscherichia coliSwineEnterotoxigenic Escherichia coli,DiarrheaAssociação entre fatores de virulência e resistência antimicrobiana de Escherichia coli enterotoxigênicas isoladas de leitões com diarreia no BrasilAssociation between virulence factors and antimicrobial resistance from enterotoxigenic escherichia coli isolated from pigs with diarrhea in Brazil info:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000985282.pdf.txt000985282.pdf.txtExtracted Texttext/plain29178http://www.lume.ufrgs.br/bitstream/10183/223833/2/000985282.pdf.txtdb66d41fc672cf9f7308850ee912a152MD52ORIGINAL000985282.pdfTexto completoapplication/pdf146209http://www.lume.ufrgs.br/bitstream/10183/223833/1/000985282.pdf471f9f5dcdba54c9edbbc8cee9d6bac0MD5110183/2238332021-08-18 04:33:32.597347oai:www.lume.ufrgs.br:10183/223833Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-08-18T07:33:32Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Associação entre fatores de virulência e resistência antimicrobiana de Escherichia coli enterotoxigênicas isoladas de leitões com diarreia no Brasil |
dc.title.alternative.en.fl_str_mv |
Association between virulence factors and antimicrobial resistance from enterotoxigenic escherichia coli isolated from pigs with diarrhea in Brazil |
title |
Associação entre fatores de virulência e resistência antimicrobiana de Escherichia coli enterotoxigênicas isoladas de leitões com diarreia no Brasil |
spellingShingle |
Associação entre fatores de virulência e resistência antimicrobiana de Escherichia coli enterotoxigênicas isoladas de leitões com diarreia no Brasil Sato, José Paulo Hiroji Suíno Diarréia Escherichia coli Swine Enterotoxigenic Escherichia coli, Diarrhea |
title_short |
Associação entre fatores de virulência e resistência antimicrobiana de Escherichia coli enterotoxigênicas isoladas de leitões com diarreia no Brasil |
title_full |
Associação entre fatores de virulência e resistência antimicrobiana de Escherichia coli enterotoxigênicas isoladas de leitões com diarreia no Brasil |
title_fullStr |
Associação entre fatores de virulência e resistência antimicrobiana de Escherichia coli enterotoxigênicas isoladas de leitões com diarreia no Brasil |
title_full_unstemmed |
Associação entre fatores de virulência e resistência antimicrobiana de Escherichia coli enterotoxigênicas isoladas de leitões com diarreia no Brasil |
title_sort |
Associação entre fatores de virulência e resistência antimicrobiana de Escherichia coli enterotoxigênicas isoladas de leitões com diarreia no Brasil |
author |
Sato, José Paulo Hiroji |
author_facet |
Sato, José Paulo Hiroji Takeuti, Karine Ludwig Daniel, Amanda Gabrielle de Souza Koerich, Priscilla K.V. Bernardi, Mari Lourdes Barcellos, David Emilio Santos Neves de |
author_role |
author |
author2 |
Takeuti, Karine Ludwig Daniel, Amanda Gabrielle de Souza Koerich, Priscilla K.V. Bernardi, Mari Lourdes Barcellos, David Emilio Santos Neves de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Sato, José Paulo Hiroji Takeuti, Karine Ludwig Daniel, Amanda Gabrielle de Souza Koerich, Priscilla K.V. Bernardi, Mari Lourdes Barcellos, David Emilio Santos Neves de |
dc.subject.por.fl_str_mv |
Suíno Diarréia Escherichia coli |
topic |
Suíno Diarréia Escherichia coli Swine Enterotoxigenic Escherichia coli, Diarrhea |
dc.subject.eng.fl_str_mv |
Swine Enterotoxigenic Escherichia coli, Diarrhea |
description |
Background: Enterotoxigenic Escherichia coli (ETEC) is the etiologic agent of post weaning colibacillosis, one of the most important diseases in pig farming. The pathogenesis of the disease is associated with two virulence factors (VF), fimbriae and enterotoxins. In veterinary medicine, the use of antibiotics can lead to the selection of resistant bacteria. The association of VF and antibiotic resistance is an important mechanism for bacterial survival under adverse conditions. This study aimed to determine the VF and antimicrobial susceptibility of ETEC isolates from piglets with diarrhea and analyze the association between these factors. Materials, Methods & Results: A total of 185 rectal swabs were collected from weaned piglets in Brazilian farms of the states of Rio Grande do Sul, Santa Catarina, Paraná, Minas Gerais and Goiás. The isolation of ETEC was carried out on blood and MacConkey Agar and characterization by biochemical tests and detection by PCR of fimbrial genes F4, F45, F6, F18 and F41, and toxins genes LT, STa, STb and STx2e. Antimicrobial susceptibility profiles were determined by Agar diffusion test for amoxicillin, ampicillin, ceftiofur, ciprofloxacin, colistin, doxycycline, enrofloxacin, florfenicol, gentamicin, neomycin, norfloxacin, oxytetracycline, streptomycin, tetracycline, lincomycin + spectinomycin and sulfamethoxazole + trimethoprim. The association between VF and antimicrobials resistance results was determined by Chi-square and Fisher test (P ≤ 0.05). A total of 376 isolates were analyzed. The frequencies of fimbriae and toxins amplified were: F4 (31.6%), F18 (18.9%), F5 (4.2%) and toxins STa (43.1%), STb (24.7%), LT (21.8%) and STx2e (5.3%). Antibiotic resistance was higher to tetracycline (96.3%), florfenicol (95.2%), oxytetracycline (93.62%) and doxycycline (90.7%). Lowest levels of resistance were to ceftiofur (2.1%), colistin (9.8%), lincomycin + spectinomicin (15.4%) and neomycin (23.1%). The association of VF and resistance was significant for fimbriae F4 and streptomycin and sulfamethoxazole + trimethoprim; F5 and enrofloxacin, ciprofloxacin and norfloxacin; F18 and amoxicillin, ampicillin, doxycycline, enrofloxacin, norfloxacin, ciprofloxacin and sulfamethoxazole + trimethoprim. Among the toxins LT and tetracycline, streptomycin and florfenicol; STa and amoxicillin, ampicillin, oxytetracycline, enrofloxacin and sulfamethoxazole + trimethoprim; STb and colistin and streptomycin. Discussion: Resistance to all antimicrobials was observed, with higher levels for tetracyclines and lower to ceftiofur and lincomycin + spectinomycin. Several studies have reported ETEC virotype variation which can be influenced by management differences in pig farms. The presence of VF and acquisition of antimicrobial resistance mechanisms might be related to evolutionary factors, infection pressure and intensive use of antibiotics. E. coli is considered one of the most versatile bacterial species and its diversity in adaptability is due to its genomic plasticity, influencing the capacity to colonize numerous host species. This is possible by mechanisms such as gain or loss of genes through lateral transfer of plasmids, transposons and integrons, which vary regarding the environment to which they are exposed. More studies are needed to correlate genetically the interaction of antimicrobial resistance and virulence genes to elucidate if the virulence expression is affected by chromosomal mutations that lead to specific resistance or/ and both determinants are inserted into the same mobile genetic element, such as a conjugative plasmid. |
publishDate |
2015 |
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2015 |
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2021-07-14T04:31:01Z |
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Acta scientiae veterinariae. Porto Alegre, RS. Vol. 43, (2015), [7 p.], Pub. 1329 |
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