Experimental model of hepatic steatosis by fructose in adult zebrafish : a pilot study

• Autor(a) principal: Ferrari, Jéssica Tonin
• Data de Publicação: 2018
• Outros Autores: Ayres, Raquel, Hammes, Thais Ortiz, Silveira, Themis Reverbel da, Cruz, Carolina Uribe
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/182145
• topic: Figado gorduroso; Peixe-zebra; Frutose; Modelos animais; Fatty liver; Fructose; Zebrafish

The consumption of fructose has been questioned, since its increase has led to an associated increase in steatosis caused by nonalcoholic fatty liver disease. Despite the advantages presented by the zebrafish as an animal model, at present there are no models of steatosis by fructose in adult zebrafish. The aim of this study is to establish a model of hepatic steatosis by fructose in adult zebrafish. Methods: Firstly, adult zebrafish were daily exposed to 4% or 6% fructose. Then, animals were exposed to 6% fructose every 2 days. The hepatic lipid accumulation was analyzed by Nile Red and Oil Red O staining. Results: The daily exposure to 6% fructose showed increased accumulation of hepatic lipids when compared to 4% and control groups, but the same concentration showed no difference when the exposure happened every 2 days. Conclusion: We can suggest the daily exposure to a concentration of 6% fructose can be considered as a new experimental model of adult zebrafish.