Optimal conditions for continuous immobilization of Pseudozyma hubeiensis (Strain HB85A) lipase by adsorption in a packed-bed reactor by response surface methodology

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Roberta da Silva Bussamara
Data de Publicação: 2012
Outros Autores: Dall'Agnol, Luciane, Schrank, Augusto, Fernandes, Kátia Flávia, Vainstein, Marilene Henning
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/225861
Resumo: This study aimed to develop an optimal continuous process for lipase immobilization in a bed reactor in order to investigate the possibility of large-scale production. An extracellular lipase of Pseudozyma hubeiensis (strain HB85A) was immobilized by adsorption onto a polystyrene-divinylbenzene support. Furthermore, response surface methodology (RSM) was employed to optimize enzyme immobilization and evaluate the optimum temperature and pH for free and immobilized enzyme. The optimal immobilization conditions observed were 150 min incubation time, pH 4.76, and an enzyme/support ratio of 1282 U/g support. Optimal activity temperature for free and immobilized enzyme was found to be 68◦C and 52◦C, respectively. Optimal activity pH for free and immobilized lipase was pH 4.6 and 6.0, respectively. Lipase immobilization resulted in improved enzyme stability in the presence of nonionic detergents, at high temp
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spelling Rodrigues, Roberta da Silva BussamaraDall'Agnol, LucianeSchrank, AugustoFernandes, Kátia FláviaVainstein, Marilene Henning2021-08-18T04:46:20Z20122090-0414http://hdl.handle.net/10183/225861000922362This study aimed to develop an optimal continuous process for lipase immobilization in a bed reactor in order to investigate the possibility of large-scale production. An extracellular lipase of Pseudozyma hubeiensis (strain HB85A) was immobilized by adsorption onto a polystyrene-divinylbenzene support. Furthermore, response surface methodology (RSM) was employed to optimize enzyme immobilization and evaluate the optimum temperature and pH for free and immobilized enzyme. The optimal immobilization conditions observed were 150 min incubation time, pH 4.76, and an enzyme/support ratio of 1282 U/g support. Optimal activity temperature for free and immobilized enzyme was found to be 68◦C and 52◦C, respectively. Optimal activity pH for free and immobilized lipase was pH 4.6 and 6.0, respectively. Lipase immobilization resulted in improved enzyme stability in the presence of nonionic detergents, at high tempapplication/pdfengEnzyme Research. New York, US. Vol. 2012, (2012), p. 1-12Pseudozyma hubeiensisLipaseOptimal conditions for continuous immobilization of Pseudozyma hubeiensis (Strain HB85A) lipase by adsorption in a packed-bed reactor by response surface methodologyEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000922362.pdf.txt000922362.pdf.txtExtracted Texttext/plain55530http://www.lume.ufrgs.br/bitstream/10183/225861/2/000922362.pdf.txt02c77ad8108e8d2c3545465c9a98a5e5MD52ORIGINAL000922362.pdfTexto completo (inglês)application/pdf2177635http://www.lume.ufrgs.br/bitstream/10183/225861/1/000922362.pdfaa22c1da174de8b62a8f2f164bf86e9dMD5110183/2258612021-08-18 05:21:36.047oai:www.lume.ufrgs.br:10183/225861Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-08-18T08:21:36Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Optimal conditions for continuous immobilization of Pseudozyma hubeiensis (Strain HB85A) lipase by adsorption in a packed-bed reactor by response surface methodology
title Optimal conditions for continuous immobilization of Pseudozyma hubeiensis (Strain HB85A) lipase by adsorption in a packed-bed reactor by response surface methodology
spellingShingle Optimal conditions for continuous immobilization of Pseudozyma hubeiensis (Strain HB85A) lipase by adsorption in a packed-bed reactor by response surface methodology
Rodrigues, Roberta da Silva Bussamara
Pseudozyma hubeiensis
Lipase
title_short Optimal conditions for continuous immobilization of Pseudozyma hubeiensis (Strain HB85A) lipase by adsorption in a packed-bed reactor by response surface methodology
title_full Optimal conditions for continuous immobilization of Pseudozyma hubeiensis (Strain HB85A) lipase by adsorption in a packed-bed reactor by response surface methodology
title_fullStr Optimal conditions for continuous immobilization of Pseudozyma hubeiensis (Strain HB85A) lipase by adsorption in a packed-bed reactor by response surface methodology
title_full_unstemmed Optimal conditions for continuous immobilization of Pseudozyma hubeiensis (Strain HB85A) lipase by adsorption in a packed-bed reactor by response surface methodology
title_sort Optimal conditions for continuous immobilization of Pseudozyma hubeiensis (Strain HB85A) lipase by adsorption in a packed-bed reactor by response surface methodology
author Rodrigues, Roberta da Silva Bussamara
author_facet Rodrigues, Roberta da Silva Bussamara
Dall'Agnol, Luciane
Schrank, Augusto
Fernandes, Kátia Flávia
Vainstein, Marilene Henning
author_role author
author2 Dall'Agnol, Luciane
Schrank, Augusto
Fernandes, Kátia Flávia
Vainstein, Marilene Henning
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rodrigues, Roberta da Silva Bussamara
Dall'Agnol, Luciane
Schrank, Augusto
Fernandes, Kátia Flávia
Vainstein, Marilene Henning
dc.subject.por.fl_str_mv Pseudozyma hubeiensis
Lipase
topic Pseudozyma hubeiensis
Lipase
description This study aimed to develop an optimal continuous process for lipase immobilization in a bed reactor in order to investigate the possibility of large-scale production. An extracellular lipase of Pseudozyma hubeiensis (strain HB85A) was immobilized by adsorption onto a polystyrene-divinylbenzene support. Furthermore, response surface methodology (RSM) was employed to optimize enzyme immobilization and evaluate the optimum temperature and pH for free and immobilized enzyme. The optimal immobilization conditions observed were 150 min incubation time, pH 4.76, and an enzyme/support ratio of 1282 U/g support. Optimal activity temperature for free and immobilized enzyme was found to be 68◦C and 52◦C, respectively. Optimal activity pH for free and immobilized lipase was pH 4.6 and 6.0, respectively. Lipase immobilization resulted in improved enzyme stability in the presence of nonionic detergents, at high temp
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2021-08-18T04:46:20Z
dc.type.driver.fl_str_mv Estrangeiro
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/225861
dc.identifier.issn.pt_BR.fl_str_mv 2090-0414
dc.identifier.nrb.pt_BR.fl_str_mv 000922362
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dc.relation.ispartof.pt_BR.fl_str_mv Enzyme Research. New York, US. Vol. 2012, (2012), p. 1-12
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