Enriched environment prevents oxidative stress in zebrafish submitted to unpredictable chronic stress
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/224238 |
Resumo: | Background. The enriched environment (EE) is a laboratory housing model that emerged from efforts to minimize the impact of environmental conditions on laboratory animals. Recently, we showed that EE promoted positive effects on behavior and cortisol levels in zebrafish submitted to the unpredictable chronic stress (UCS) protocol. Here, we expanded the characterization of the effects of UCS protocol by assessing parameters of oxidative status in the zebrafish brain and reveal that EE protects against the oxidative stress induced by chronic stress. Methods. Zebrafish were exposed to EE (21 or 28 days) or standard housing conditions and subjected to the UCS protocol for seven days. Oxidative stress parameters (lipid peroxidation (TBARS), reactive oxygen species (ROS) levels, non-protein thiol (NPSH) and total thiol (SH) levels, superoxide dismutase (SOD) and catalase (CAT) activities were measured in brain homogenate. Results. Our results revealed that UCS increased lipid peroxidation and ROS levels, while decreased NPSH levels and SOD activity, suggesting oxidative damage. EE for 28 days prevented all changes induced by the UCS protocol, and EE for 21 days prevented the alterations on NPSH levels, lipid peroxidation and ROS levels. Both EE for 21 or 28 days increased CAT activity. Discussion. Our findings reinforce the idea that EE exerts neuromodulatory effects in the zebrafish brain. EE promoted positive effects as it helped maintain the redox homeostasis, which may reduce the susceptibility to stress and its oxidative impact. |
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Marcon, Matheus FelipeMocelin, Ricieri NaueSachett, AdrieliSiebel, Anna MariaHerrmann, Ana PaulaPiato, Angelo Luis Stapassoli2021-07-21T04:23:40Z20182167-8359http://hdl.handle.net/10183/224238001077038Background. The enriched environment (EE) is a laboratory housing model that emerged from efforts to minimize the impact of environmental conditions on laboratory animals. Recently, we showed that EE promoted positive effects on behavior and cortisol levels in zebrafish submitted to the unpredictable chronic stress (UCS) protocol. Here, we expanded the characterization of the effects of UCS protocol by assessing parameters of oxidative status in the zebrafish brain and reveal that EE protects against the oxidative stress induced by chronic stress. Methods. Zebrafish were exposed to EE (21 or 28 days) or standard housing conditions and subjected to the UCS protocol for seven days. Oxidative stress parameters (lipid peroxidation (TBARS), reactive oxygen species (ROS) levels, non-protein thiol (NPSH) and total thiol (SH) levels, superoxide dismutase (SOD) and catalase (CAT) activities were measured in brain homogenate. Results. Our results revealed that UCS increased lipid peroxidation and ROS levels, while decreased NPSH levels and SOD activity, suggesting oxidative damage. EE for 28 days prevented all changes induced by the UCS protocol, and EE for 21 days prevented the alterations on NPSH levels, lipid peroxidation and ROS levels. Both EE for 21 or 28 days increased CAT activity. Discussion. Our findings reinforce the idea that EE exerts neuromodulatory effects in the zebrafish brain. EE promoted positive effects as it helped maintain the redox homeostasis, which may reduce the susceptibility to stress and its oxidative impact.application/pdfengPeerJ. Corte Madera. Vol. 6 (2018), e5136, 15 p.Estresse oxidativoPeixe-zebraNeuroproteçãoComportamento animalEnvironmental enrichmentUnpredictable chronic stressOxidative stressZebrafishEnriched environment prevents oxidative stress in zebrafish submitted to unpredictable chronic stressEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001077038.pdf.txt001077038.pdf.txtExtracted Texttext/plain40548http://www.lume.ufrgs.br/bitstream/10183/224238/2/001077038.pdf.txt56b8644893f1862424eeee03597168dbMD52ORIGINAL001077038.pdfTexto completo (inglês)application/pdf7489854http://www.lume.ufrgs.br/bitstream/10183/224238/1/001077038.pdfce890daf7493baa7fb30f557e69084a1MD5110183/2242382021-08-18 04:32:53.711707oai:www.lume.ufrgs.br:10183/224238Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-08-18T07:32:53Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Enriched environment prevents oxidative stress in zebrafish submitted to unpredictable chronic stress |
title |
Enriched environment prevents oxidative stress in zebrafish submitted to unpredictable chronic stress |
spellingShingle |
Enriched environment prevents oxidative stress in zebrafish submitted to unpredictable chronic stress Marcon, Matheus Felipe Estresse oxidativo Peixe-zebra Neuroproteção Comportamento animal Environmental enrichment Unpredictable chronic stress Oxidative stress Zebrafish |
title_short |
Enriched environment prevents oxidative stress in zebrafish submitted to unpredictable chronic stress |
title_full |
Enriched environment prevents oxidative stress in zebrafish submitted to unpredictable chronic stress |
title_fullStr |
Enriched environment prevents oxidative stress in zebrafish submitted to unpredictable chronic stress |
title_full_unstemmed |
Enriched environment prevents oxidative stress in zebrafish submitted to unpredictable chronic stress |
title_sort |
Enriched environment prevents oxidative stress in zebrafish submitted to unpredictable chronic stress |
author |
Marcon, Matheus Felipe |
author_facet |
Marcon, Matheus Felipe Mocelin, Ricieri Naue Sachett, Adrieli Siebel, Anna Maria Herrmann, Ana Paula Piato, Angelo Luis Stapassoli |
author_role |
author |
author2 |
Mocelin, Ricieri Naue Sachett, Adrieli Siebel, Anna Maria Herrmann, Ana Paula Piato, Angelo Luis Stapassoli |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Marcon, Matheus Felipe Mocelin, Ricieri Naue Sachett, Adrieli Siebel, Anna Maria Herrmann, Ana Paula Piato, Angelo Luis Stapassoli |
dc.subject.por.fl_str_mv |
Estresse oxidativo Peixe-zebra Neuroproteção Comportamento animal |
topic |
Estresse oxidativo Peixe-zebra Neuroproteção Comportamento animal Environmental enrichment Unpredictable chronic stress Oxidative stress Zebrafish |
dc.subject.eng.fl_str_mv |
Environmental enrichment Unpredictable chronic stress Oxidative stress Zebrafish |
description |
Background. The enriched environment (EE) is a laboratory housing model that emerged from efforts to minimize the impact of environmental conditions on laboratory animals. Recently, we showed that EE promoted positive effects on behavior and cortisol levels in zebrafish submitted to the unpredictable chronic stress (UCS) protocol. Here, we expanded the characterization of the effects of UCS protocol by assessing parameters of oxidative status in the zebrafish brain and reveal that EE protects against the oxidative stress induced by chronic stress. Methods. Zebrafish were exposed to EE (21 or 28 days) or standard housing conditions and subjected to the UCS protocol for seven days. Oxidative stress parameters (lipid peroxidation (TBARS), reactive oxygen species (ROS) levels, non-protein thiol (NPSH) and total thiol (SH) levels, superoxide dismutase (SOD) and catalase (CAT) activities were measured in brain homogenate. Results. Our results revealed that UCS increased lipid peroxidation and ROS levels, while decreased NPSH levels and SOD activity, suggesting oxidative damage. EE for 28 days prevented all changes induced by the UCS protocol, and EE for 21 days prevented the alterations on NPSH levels, lipid peroxidation and ROS levels. Both EE for 21 or 28 days increased CAT activity. Discussion. Our findings reinforce the idea that EE exerts neuromodulatory effects in the zebrafish brain. EE promoted positive effects as it helped maintain the redox homeostasis, which may reduce the susceptibility to stress and its oxidative impact. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2021-07-21T04:23:40Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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article |
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http://hdl.handle.net/10183/224238 |
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2167-8359 |
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001077038 |
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http://hdl.handle.net/10183/224238 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
PeerJ. Corte Madera. Vol. 6 (2018), e5136, 15 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Repositório Institucional da UFRGS |
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