Aging and its impact onthe quality of grafts : an experimental study in rats livers
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/88209 |
Resumo: | There are consistent clues of worse results with grafts from older donors, especially in hepatitis C. University of Wisconsin (UW) solution is adopted for liver preservation, but other solutions are being studied, as fructose-1,6-bisphosphate (FBP). Objective – To determinate the impact of aging of the donor on the cold ischemia injury in rat livers and compare UW and FBP. Methods – Twenty male Wistar rats were studied - 10, aging 20 weeks: 5 to preservation with UW (C-UW) and 5, FBP (C-FBP); and other 10, aging 50 weeks: 5 to UW (E-UW) and 5 to FBP (E-FBP). Rats were anesthesized, submitted to hepatectomy, and graft was kept under 2-4ºC for 8 hours. Liquid samples were taken at 2, 4, 6, and 8 hours, to determine AST and LDH. At the end, in the liver tissue thiobarbituric acid reactive substances and catalase were determined. Results - Average liver weight was similar between controls and the others (12.5 g ± 1.8 and 13.4 g ± 2.0). AST and LDH levels were higher in C-UW group (P<0.05). In the older group, there was a difference between UW and FBP preserved livers related to LDH, but not to AST. Thiobarbituric acid reactive substances were superior in control group than in the older one (P = 0.001). Catalase activity was similar between these groups (P = 0.11), but it was superior in UW preserved animals (P = 0.02). Conclusion - Livers from older rats were similar to the controls regarding cold ischemia injury in FBP group. Surprisingly, with UW solution there was less cold ischemia injury in the elderly group. When comparing both solutions, FBP provided significantly more protection than UW in the controls. There was a trend to FBP to being better than UW in the elderly group. Further studies with liver from older donors and ischemia and reperfusion are needed. |
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Mota, Stela MariaGasperin, GlauberCerski, Carlos Thadeu SchmidtOliveira, Jarbas Rodrigues deÁlvares-da-Silva, Mário Reis2014-03-07T01:50:04Z20080004-2803http://hdl.handle.net/10183/88209000837297There are consistent clues of worse results with grafts from older donors, especially in hepatitis C. University of Wisconsin (UW) solution is adopted for liver preservation, but other solutions are being studied, as fructose-1,6-bisphosphate (FBP). Objective – To determinate the impact of aging of the donor on the cold ischemia injury in rat livers and compare UW and FBP. Methods – Twenty male Wistar rats were studied - 10, aging 20 weeks: 5 to preservation with UW (C-UW) and 5, FBP (C-FBP); and other 10, aging 50 weeks: 5 to UW (E-UW) and 5 to FBP (E-FBP). Rats were anesthesized, submitted to hepatectomy, and graft was kept under 2-4ºC for 8 hours. Liquid samples were taken at 2, 4, 6, and 8 hours, to determine AST and LDH. At the end, in the liver tissue thiobarbituric acid reactive substances and catalase were determined. Results - Average liver weight was similar between controls and the others (12.5 g ± 1.8 and 13.4 g ± 2.0). AST and LDH levels were higher in C-UW group (P<0.05). In the older group, there was a difference between UW and FBP preserved livers related to LDH, but not to AST. Thiobarbituric acid reactive substances were superior in control group than in the older one (P = 0.001). Catalase activity was similar between these groups (P = 0.11), but it was superior in UW preserved animals (P = 0.02). Conclusion - Livers from older rats were similar to the controls regarding cold ischemia injury in FBP group. Surprisingly, with UW solution there was less cold ischemia injury in the elderly group. When comparing both solutions, FBP provided significantly more protection than UW in the controls. There was a trend to FBP to being better than UW in the elderly group. Further studies with liver from older donors and ischemia and reperfusion are needed.application/pdfporArquivos de gastroenterologia = Archives of gastroenterology. São Paulo. Vol. 47, n. 3 (jul./set. 2010), p. 297-300Modelos animaisPreservação de órgãosMétodosSoluções para preservação de órgãosAdenosinaAlopurinolCriopreservaçãoGlutationaInsulinaAgingLiver transplantationTissue donorsRatsAging and its impact onthe quality of grafts : an experimental study in rats liversO impacto do envelhecimento na qualidade do enxerto : estudo experimental em fígados de ratosinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000837297.pdf000837297.pdfTexto completoapplication/pdf233697http://www.lume.ufrgs.br/bitstream/10183/88209/1/000837297.pdf38a77b7db1baf96c3fbe8de253d54618MD51TEXT000837297.pdf.txt000837297.pdf.txtExtracted Texttext/plain19475http://www.lume.ufrgs.br/bitstream/10183/88209/2/000837297.pdf.txt86d2648ba9a403d29da12dd9646cedc2MD52THUMBNAIL000837297.pdf.jpg000837297.pdf.jpgGenerated Thumbnailimage/jpeg1964http://www.lume.ufrgs.br/bitstream/10183/88209/3/000837297.pdf.jpg3164754ecc556b157d8f3a782f052a8cMD5310183/882092023-11-01 03:25:30.494167oai:www.lume.ufrgs.br:10183/88209Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-11-01T06:25:30Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Aging and its impact onthe quality of grafts : an experimental study in rats livers |
dc.title.alternative.pt.fl_str_mv |
O impacto do envelhecimento na qualidade do enxerto : estudo experimental em fígados de ratos |
title |
Aging and its impact onthe quality of grafts : an experimental study in rats livers |
spellingShingle |
Aging and its impact onthe quality of grafts : an experimental study in rats livers Mota, Stela Maria Modelos animais Preservação de órgãos Métodos Soluções para preservação de órgãos Adenosina Alopurinol Criopreservação Glutationa Insulina Aging Liver transplantation Tissue donors Rats |
title_short |
Aging and its impact onthe quality of grafts : an experimental study in rats livers |
title_full |
Aging and its impact onthe quality of grafts : an experimental study in rats livers |
title_fullStr |
Aging and its impact onthe quality of grafts : an experimental study in rats livers |
title_full_unstemmed |
Aging and its impact onthe quality of grafts : an experimental study in rats livers |
title_sort |
Aging and its impact onthe quality of grafts : an experimental study in rats livers |
author |
Mota, Stela Maria |
author_facet |
Mota, Stela Maria Gasperin, Glauber Cerski, Carlos Thadeu Schmidt Oliveira, Jarbas Rodrigues de Álvares-da-Silva, Mário Reis |
author_role |
author |
author2 |
Gasperin, Glauber Cerski, Carlos Thadeu Schmidt Oliveira, Jarbas Rodrigues de Álvares-da-Silva, Mário Reis |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Mota, Stela Maria Gasperin, Glauber Cerski, Carlos Thadeu Schmidt Oliveira, Jarbas Rodrigues de Álvares-da-Silva, Mário Reis |
dc.subject.por.fl_str_mv |
Modelos animais Preservação de órgãos Métodos Soluções para preservação de órgãos Adenosina Alopurinol Criopreservação Glutationa Insulina |
topic |
Modelos animais Preservação de órgãos Métodos Soluções para preservação de órgãos Adenosina Alopurinol Criopreservação Glutationa Insulina Aging Liver transplantation Tissue donors Rats |
dc.subject.eng.fl_str_mv |
Aging Liver transplantation Tissue donors Rats |
description |
There are consistent clues of worse results with grafts from older donors, especially in hepatitis C. University of Wisconsin (UW) solution is adopted for liver preservation, but other solutions are being studied, as fructose-1,6-bisphosphate (FBP). Objective – To determinate the impact of aging of the donor on the cold ischemia injury in rat livers and compare UW and FBP. Methods – Twenty male Wistar rats were studied - 10, aging 20 weeks: 5 to preservation with UW (C-UW) and 5, FBP (C-FBP); and other 10, aging 50 weeks: 5 to UW (E-UW) and 5 to FBP (E-FBP). Rats were anesthesized, submitted to hepatectomy, and graft was kept under 2-4ºC for 8 hours. Liquid samples were taken at 2, 4, 6, and 8 hours, to determine AST and LDH. At the end, in the liver tissue thiobarbituric acid reactive substances and catalase were determined. Results - Average liver weight was similar between controls and the others (12.5 g ± 1.8 and 13.4 g ± 2.0). AST and LDH levels were higher in C-UW group (P<0.05). In the older group, there was a difference between UW and FBP preserved livers related to LDH, but not to AST. Thiobarbituric acid reactive substances were superior in control group than in the older one (P = 0.001). Catalase activity was similar between these groups (P = 0.11), but it was superior in UW preserved animals (P = 0.02). Conclusion - Livers from older rats were similar to the controls regarding cold ischemia injury in FBP group. Surprisingly, with UW solution there was less cold ischemia injury in the elderly group. When comparing both solutions, FBP provided significantly more protection than UW in the controls. There was a trend to FBP to being better than UW in the elderly group. Further studies with liver from older donors and ischemia and reperfusion are needed. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008 |
dc.date.accessioned.fl_str_mv |
2014-03-07T01:50:04Z |
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http://hdl.handle.net/10183/88209 |
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0004-2803 |
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000837297 |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Arquivos de gastroenterologia = Archives of gastroenterology. São Paulo. Vol. 47, n. 3 (jul./set. 2010), p. 297-300 |
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