Rifampicin : biotransformation study using the fungus Cunninghamella elegans and monitoring through UHPLC-MS

Detalhes bibliográficos
Autor(a) principal: Sponchiado, Rafaela Martins
Data de Publicação: 2020
Outros Autores: Sorrentino, Julia Medeiros, Cordenonsi, Leticia Malgarim, Fuentefria, Alexandre Meneghello, Steppe, Martin, Mendez, Andreas Sebastian Loureiro, Garcia, Cassia Virginia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/231508
Resumo: Drug biotransformation studies appear as an alternative to pharmacological investigations of metabolites, development of new drug candidates with reduced investment and most efficient production. The objective of this study was to evaluate the capacity of biotransformation of Rifampicin (RIF) by the filamentous fungus Cunninghamella elegans as a microbial model of mammalian metabolism. In 120 h, C. elegans transformed the drug into the following two metabolites: rifampicin quinone and novel metabolite. The products of rifampicin formed in vitro were monitored by HPLC-PDA, being identified through UHPLC–QTOF/MS. Metabolites were characterized according to their chromatographic profile, mass fragments and UV spectral data. The major metabolic pathways of rifampicin transformed by the fungus were oxidation, demethylation and mono-oxidation. The microbial transformation of RIF showed the potential of Cunninghamella species to produce RIF metabolites. This process can be used for a cost effective method for both known and unknown metabolite production.
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spelling Sponchiado, Rafaela MartinsSorrentino, Julia MedeirosCordenonsi, Leticia MalgarimFuentefria, Alexandre MeneghelloSteppe, MartinMendez, Andreas Sebastian LoureiroGarcia, Cassia Virginia2021-11-04T04:23:51Z20202527-2616http://hdl.handle.net/10183/231508001124248Drug biotransformation studies appear as an alternative to pharmacological investigations of metabolites, development of new drug candidates with reduced investment and most efficient production. The objective of this study was to evaluate the capacity of biotransformation of Rifampicin (RIF) by the filamentous fungus Cunninghamella elegans as a microbial model of mammalian metabolism. In 120 h, C. elegans transformed the drug into the following two metabolites: rifampicin quinone and novel metabolite. The products of rifampicin formed in vitro were monitored by HPLC-PDA, being identified through UHPLC–QTOF/MS. Metabolites were characterized according to their chromatographic profile, mass fragments and UV spectral data. The major metabolic pathways of rifampicin transformed by the fungus were oxidation, demethylation and mono-oxidation. The microbial transformation of RIF showed the potential of Cunninghamella species to produce RIF metabolites. This process can be used for a cost effective method for both known and unknown metabolite production.application/pdfengDrug analytical research. Porto Alegre (RS). Vol. 4, n. 1 (2020), p. 44-48FarmáciaRifampicinaBiotransformacaoCunninghamellaBiotransformationNew metaboliteCunninghamella elegansUHPLC-MSRifampicin : biotransformation study using the fungus Cunninghamella elegans and monitoring through UHPLC-MSinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001124248.pdf.txt001124248.pdf.txtExtracted Texttext/plain20597http://www.lume.ufrgs.br/bitstream/10183/231508/2/001124248.pdf.txt98662af938d357fc7721f3090db4c7c4MD52ORIGINAL001124248.pdfTexto completo (inglês)application/pdf418681http://www.lume.ufrgs.br/bitstream/10183/231508/1/001124248.pdf496c27c068b0dc8a43d9cbc66e71f4c5MD5110183/2315082021-11-20 05:46:49.7898oai:www.lume.ufrgs.br:10183/231508Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-11-20T07:46:49Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Rifampicin : biotransformation study using the fungus Cunninghamella elegans and monitoring through UHPLC-MS
title Rifampicin : biotransformation study using the fungus Cunninghamella elegans and monitoring through UHPLC-MS
spellingShingle Rifampicin : biotransformation study using the fungus Cunninghamella elegans and monitoring through UHPLC-MS
Sponchiado, Rafaela Martins
Farmácia
Rifampicina
Biotransformacao
Cunninghamella
Biotransformation
New metabolite
Cunninghamella elegans
UHPLC-MS
title_short Rifampicin : biotransformation study using the fungus Cunninghamella elegans and monitoring through UHPLC-MS
title_full Rifampicin : biotransformation study using the fungus Cunninghamella elegans and monitoring through UHPLC-MS
title_fullStr Rifampicin : biotransformation study using the fungus Cunninghamella elegans and monitoring through UHPLC-MS
title_full_unstemmed Rifampicin : biotransformation study using the fungus Cunninghamella elegans and monitoring through UHPLC-MS
title_sort Rifampicin : biotransformation study using the fungus Cunninghamella elegans and monitoring through UHPLC-MS
author Sponchiado, Rafaela Martins
author_facet Sponchiado, Rafaela Martins
Sorrentino, Julia Medeiros
Cordenonsi, Leticia Malgarim
Fuentefria, Alexandre Meneghello
Steppe, Martin
Mendez, Andreas Sebastian Loureiro
Garcia, Cassia Virginia
author_role author
author2 Sorrentino, Julia Medeiros
Cordenonsi, Leticia Malgarim
Fuentefria, Alexandre Meneghello
Steppe, Martin
Mendez, Andreas Sebastian Loureiro
Garcia, Cassia Virginia
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sponchiado, Rafaela Martins
Sorrentino, Julia Medeiros
Cordenonsi, Leticia Malgarim
Fuentefria, Alexandre Meneghello
Steppe, Martin
Mendez, Andreas Sebastian Loureiro
Garcia, Cassia Virginia
dc.subject.por.fl_str_mv Farmácia
Rifampicina
Biotransformacao
Cunninghamella
topic Farmácia
Rifampicina
Biotransformacao
Cunninghamella
Biotransformation
New metabolite
Cunninghamella elegans
UHPLC-MS
dc.subject.eng.fl_str_mv Biotransformation
New metabolite
Cunninghamella elegans
UHPLC-MS
description Drug biotransformation studies appear as an alternative to pharmacological investigations of metabolites, development of new drug candidates with reduced investment and most efficient production. The objective of this study was to evaluate the capacity of biotransformation of Rifampicin (RIF) by the filamentous fungus Cunninghamella elegans as a microbial model of mammalian metabolism. In 120 h, C. elegans transformed the drug into the following two metabolites: rifampicin quinone and novel metabolite. The products of rifampicin formed in vitro were monitored by HPLC-PDA, being identified through UHPLC–QTOF/MS. Metabolites were characterized according to their chromatographic profile, mass fragments and UV spectral data. The major metabolic pathways of rifampicin transformed by the fungus were oxidation, demethylation and mono-oxidation. The microbial transformation of RIF showed the potential of Cunninghamella species to produce RIF metabolites. This process can be used for a cost effective method for both known and unknown metabolite production.
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2021-11-04T04:23:51Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/231508
dc.identifier.issn.pt_BR.fl_str_mv 2527-2616
dc.identifier.nrb.pt_BR.fl_str_mv 001124248
identifier_str_mv 2527-2616
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url http://hdl.handle.net/10183/231508
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Drug analytical research. Porto Alegre (RS). Vol. 4, n. 1 (2020), p. 44-48
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRGS
instname:Universidade Federal do Rio Grande do Sul (UFRGS)
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instname_str Universidade Federal do Rio Grande do Sul (UFRGS)
instacron_str UFRGS
institution UFRGS
reponame_str Repositório Institucional da UFRGS
collection Repositório Institucional da UFRGS
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