Effect of hydrogel containing Achyrocline satureioides (Asteraceae) extract–loaded nanoemulsions on wound healing activity
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/253598 |
Resumo: | Achyrocline satureioides (Lam.) DC extract–loaded nanoemulsions have demonstrated potential for wound healing, with promising effects on keratinocyte proliferation. We carried out the first in vivo investigation of the wound healing activity of a hydrogel containing A. satureioides extract– loaded nanoemulsions. We prepared hydrogels by adding the gelling agent (Carbopol® Ultrez) to extract-loaded nanoemulsions (~250 nm in diameter) obtained by spontaneous emulsification. The final flavonoid content in formulation was close to 1 mg/mL, as estimated by ultra-fast liquid chromatography. Permeation/retention studies using porcine ear skin showed that flavonoids reached deeper layers of pig ear skin when it was damaged (up to 3.2 g/cm2 in the dermis), but did not reach the Franz-type diffusion cell receptor fluid. For healing activity, we performed a dorsal wound model using Wistar rats, evaluating the lesion size, anti-inflammatory markers, oxidative damage, and histology. We found that extract-loaded formulations promoted wound healing by increasing angiogenesis by ~20%, reducing inflammation (tumor necrosis factor ) by ~35%, decreasing lipid damage, and improving the re-epithelialization process in lesions. In addition, there was an increase in the number of blood vessels and hair follicles for wounds treated with the formulation compared with the controls. Our findings indicate that the proposed formulation could be promising in the search for better quality healing and tissue reconstruction. |
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Balestrin, Lucélia AlbarelloBack, Patrícia InêsMarques, Magno da SilvaAraújo, Gabriela de Moraes SoaresFalkembach, Mariana CorrêaBatista, Matheus MonteiroSilveira, Tony Leandro Rezende daRodrigues, Jamile LimaFachel, Flávia Nathiely SilveiraKoester, Leticia SchererBassani, Valquiria LinckHorn, Ana PaulaDora, Cristiana LimaTeixeira, Helder Ferreira2023-01-12T04:56:00Z20221999-4923http://hdl.handle.net/10183/253598001156356Achyrocline satureioides (Lam.) DC extract–loaded nanoemulsions have demonstrated potential for wound healing, with promising effects on keratinocyte proliferation. We carried out the first in vivo investigation of the wound healing activity of a hydrogel containing A. satureioides extract– loaded nanoemulsions. We prepared hydrogels by adding the gelling agent (Carbopol® Ultrez) to extract-loaded nanoemulsions (~250 nm in diameter) obtained by spontaneous emulsification. The final flavonoid content in formulation was close to 1 mg/mL, as estimated by ultra-fast liquid chromatography. Permeation/retention studies using porcine ear skin showed that flavonoids reached deeper layers of pig ear skin when it was damaged (up to 3.2 g/cm2 in the dermis), but did not reach the Franz-type diffusion cell receptor fluid. For healing activity, we performed a dorsal wound model using Wistar rats, evaluating the lesion size, anti-inflammatory markers, oxidative damage, and histology. We found that extract-loaded formulations promoted wound healing by increasing angiogenesis by ~20%, reducing inflammation (tumor necrosis factor ) by ~35%, decreasing lipid damage, and improving the re-epithelialization process in lesions. In addition, there was an increase in the number of blood vessels and hair follicles for wounds treated with the formulation compared with the controls. Our findings indicate that the proposed formulation could be promising in the search for better quality healing and tissue reconstruction.application/pdfengPharmaceutics. Basel. Vol. 14, n. 12 (2022), 2726, 14 p.HidrogéisCicatrizaçãoFlavonoidesNanoemulsõesAchyrocline satureioidesNanoemulsionHydrogelHealing activityEffect of hydrogel containing Achyrocline satureioides (Asteraceae) extract–loaded nanoemulsions on wound healing activityEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001156356.pdf.txt001156356.pdf.txtExtracted Texttext/plain67106http://www.lume.ufrgs.br/bitstream/10183/253598/2/001156356.pdf.txt60ccfb84d858b673648a7544d5fd9721MD52ORIGINAL001156356.pdfTexto completo (inglês)application/pdf3023742http://www.lume.ufrgs.br/bitstream/10183/253598/1/001156356.pdf7e68a89df2e80bef0aabce3f11902157MD5110183/2535982023-01-13 06:03:26.659994oai:www.lume.ufrgs.br:10183/253598Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-01-13T08:03:26Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Effect of hydrogel containing Achyrocline satureioides (Asteraceae) extract–loaded nanoemulsions on wound healing activity |
title |
Effect of hydrogel containing Achyrocline satureioides (Asteraceae) extract–loaded nanoemulsions on wound healing activity |
spellingShingle |
Effect of hydrogel containing Achyrocline satureioides (Asteraceae) extract–loaded nanoemulsions on wound healing activity Balestrin, Lucélia Albarello Hidrogéis Cicatrização Flavonoides Nanoemulsões Achyrocline satureioides Nanoemulsion Hydrogel Healing activity |
title_short |
Effect of hydrogel containing Achyrocline satureioides (Asteraceae) extract–loaded nanoemulsions on wound healing activity |
title_full |
Effect of hydrogel containing Achyrocline satureioides (Asteraceae) extract–loaded nanoemulsions on wound healing activity |
title_fullStr |
Effect of hydrogel containing Achyrocline satureioides (Asteraceae) extract–loaded nanoemulsions on wound healing activity |
title_full_unstemmed |
Effect of hydrogel containing Achyrocline satureioides (Asteraceae) extract–loaded nanoemulsions on wound healing activity |
title_sort |
Effect of hydrogel containing Achyrocline satureioides (Asteraceae) extract–loaded nanoemulsions on wound healing activity |
author |
Balestrin, Lucélia Albarello |
author_facet |
Balestrin, Lucélia Albarello Back, Patrícia Inês Marques, Magno da Silva Araújo, Gabriela de Moraes Soares Falkembach, Mariana Corrêa Batista, Matheus Monteiro Silveira, Tony Leandro Rezende da Rodrigues, Jamile Lima Fachel, Flávia Nathiely Silveira Koester, Leticia Scherer Bassani, Valquiria Linck Horn, Ana Paula Dora, Cristiana Lima Teixeira, Helder Ferreira |
author_role |
author |
author2 |
Back, Patrícia Inês Marques, Magno da Silva Araújo, Gabriela de Moraes Soares Falkembach, Mariana Corrêa Batista, Matheus Monteiro Silveira, Tony Leandro Rezende da Rodrigues, Jamile Lima Fachel, Flávia Nathiely Silveira Koester, Leticia Scherer Bassani, Valquiria Linck Horn, Ana Paula Dora, Cristiana Lima Teixeira, Helder Ferreira |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Balestrin, Lucélia Albarello Back, Patrícia Inês Marques, Magno da Silva Araújo, Gabriela de Moraes Soares Falkembach, Mariana Corrêa Batista, Matheus Monteiro Silveira, Tony Leandro Rezende da Rodrigues, Jamile Lima Fachel, Flávia Nathiely Silveira Koester, Leticia Scherer Bassani, Valquiria Linck Horn, Ana Paula Dora, Cristiana Lima Teixeira, Helder Ferreira |
dc.subject.por.fl_str_mv |
Hidrogéis Cicatrização Flavonoides Nanoemulsões |
topic |
Hidrogéis Cicatrização Flavonoides Nanoemulsões Achyrocline satureioides Nanoemulsion Hydrogel Healing activity |
dc.subject.eng.fl_str_mv |
Achyrocline satureioides Nanoemulsion Hydrogel Healing activity |
description |
Achyrocline satureioides (Lam.) DC extract–loaded nanoemulsions have demonstrated potential for wound healing, with promising effects on keratinocyte proliferation. We carried out the first in vivo investigation of the wound healing activity of a hydrogel containing A. satureioides extract– loaded nanoemulsions. We prepared hydrogels by adding the gelling agent (Carbopol® Ultrez) to extract-loaded nanoemulsions (~250 nm in diameter) obtained by spontaneous emulsification. The final flavonoid content in formulation was close to 1 mg/mL, as estimated by ultra-fast liquid chromatography. Permeation/retention studies using porcine ear skin showed that flavonoids reached deeper layers of pig ear skin when it was damaged (up to 3.2 g/cm2 in the dermis), but did not reach the Franz-type diffusion cell receptor fluid. For healing activity, we performed a dorsal wound model using Wistar rats, evaluating the lesion size, anti-inflammatory markers, oxidative damage, and histology. We found that extract-loaded formulations promoted wound healing by increasing angiogenesis by ~20%, reducing inflammation (tumor necrosis factor ) by ~35%, decreasing lipid damage, and improving the re-epithelialization process in lesions. In addition, there was an increase in the number of blood vessels and hair follicles for wounds treated with the formulation compared with the controls. Our findings indicate that the proposed formulation could be promising in the search for better quality healing and tissue reconstruction. |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022 |
dc.date.accessioned.fl_str_mv |
2023-01-12T04:56:00Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/253598 |
dc.identifier.issn.pt_BR.fl_str_mv |
1999-4923 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001156356 |
identifier_str_mv |
1999-4923 001156356 |
url |
http://hdl.handle.net/10183/253598 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Pharmaceutics. Basel. Vol. 14, n. 12 (2022), 2726, 14 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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