Modulation of the intestinal microbiota of broilers supplemented with monensin or functional oils in response to challenge by Eimeria spp.
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/256021 |
Resumo: | The objective of this study was to evaluate the effect of supplementation with 100ppm sodium monensin or 0.15% of a blend of functional oils (cashew nut oil + castor oil) on the intestinal microbiota of broilers challenged with three different Eimeria spp. The challenge was accomplished by inoculating broiler chicks with sporulated oocysts of Eimeria tenella, Eimeria acervulina, and Eimeria maxima via oral gavage. A total of 864, day-old male broiler chicks (Cobb) were randomly assigned to six treatments (eight pens/treatment; 18 broilers/ pen) in a 3 × 2 factorial arrangement, composed of three additives (control, monensin or blend), with or without Eimeria challenge. Intestinal contents was collected at 28 days of age for microbiota analysis by sequencing 16s rRNA in V3 and V4 regions using the Illumina MiSeq platform. Taxonomy was assigned through the SILVA database version 132, using the QIIME 2 software version 2019.1. No treatment effects (p > 0.05) were observed in the microbial richness at the family level estimated by Chao1 and the biodiversity assessed by Simpson’s index, except for Shannon’s index (p < 0.05). The intestinal microbiota was domi- nated by members of the order Clostridiales and Lactobacillales, followed by the families Ruminococcaceae, Bacteroidaceae, and Lactobacillaceae, regardless of treatment. When the controls were compared, in the challenged control group there was an increase in Erysi- pelotrichaceae, Lactobacillaceae, Bacteroidaceae, Streptococcaceae, and Peptostrepto- coccaceae, and a decrease in Ruminococcaceae. Similar results were found for a challenged group that received monensin, while the blend partially mitigated this variation. Therefore, the blend alleviated the impact of coccidiosis challenge on the microbiome of broilers compared to monensin. |
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Vieira, Alexandre MacielSoratto, Tatiany Aparecida TeixeiraCardinal, Kátia MariaWagner, GlauberHauptli, LucéliaLima, André Luis FerreiraDahlke, FabianoPeres Netto, DiegoMoraes, Priscila de OliveiraRibeiro, Andrea Machado Leal2023-03-18T03:32:47Z20201932-6203http://hdl.handle.net/10183/256021001164100The objective of this study was to evaluate the effect of supplementation with 100ppm sodium monensin or 0.15% of a blend of functional oils (cashew nut oil + castor oil) on the intestinal microbiota of broilers challenged with three different Eimeria spp. The challenge was accomplished by inoculating broiler chicks with sporulated oocysts of Eimeria tenella, Eimeria acervulina, and Eimeria maxima via oral gavage. A total of 864, day-old male broiler chicks (Cobb) were randomly assigned to six treatments (eight pens/treatment; 18 broilers/ pen) in a 3 × 2 factorial arrangement, composed of three additives (control, monensin or blend), with or without Eimeria challenge. Intestinal contents was collected at 28 days of age for microbiota analysis by sequencing 16s rRNA in V3 and V4 regions using the Illumina MiSeq platform. Taxonomy was assigned through the SILVA database version 132, using the QIIME 2 software version 2019.1. No treatment effects (p > 0.05) were observed in the microbial richness at the family level estimated by Chao1 and the biodiversity assessed by Simpson’s index, except for Shannon’s index (p < 0.05). The intestinal microbiota was domi- nated by members of the order Clostridiales and Lactobacillales, followed by the families Ruminococcaceae, Bacteroidaceae, and Lactobacillaceae, regardless of treatment. When the controls were compared, in the challenged control group there was an increase in Erysi- pelotrichaceae, Lactobacillaceae, Bacteroidaceae, Streptococcaceae, and Peptostrepto- coccaceae, and a decrease in Ruminococcaceae. Similar results were found for a challenged group that received monensin, while the blend partially mitigated this variation. Therefore, the blend alleviated the impact of coccidiosis challenge on the microbiome of broilers compared to monensin.application/pdfengPLoS ONE. San Francisco. Vol. 15 no. 8 (Aug. 2020), e0237118, 15 p.Nutricao animalSuplemento alimentarMonensinaFrango de corteFlora microbianaModulation of the intestinal microbiota of broilers supplemented with monensin or functional oils in response to challenge by Eimeria spp.Estrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001164100.pdf.txt001164100.pdf.txtExtracted Texttext/plain48813http://www.lume.ufrgs.br/bitstream/10183/256021/2/001164100.pdf.txt68d9c8df5b60348cc1128da355f8de74MD52ORIGINAL001164100.pdfTexto completo (inglês)application/pdf2517592http://www.lume.ufrgs.br/bitstream/10183/256021/1/001164100.pdfd148d7908b619f992379e386181fb207MD5110183/2560212023-03-19 03:35:20.474246oai:www.lume.ufrgs.br:10183/256021Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-03-19T06:35:20Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Modulation of the intestinal microbiota of broilers supplemented with monensin or functional oils in response to challenge by Eimeria spp. |
title |
Modulation of the intestinal microbiota of broilers supplemented with monensin or functional oils in response to challenge by Eimeria spp. |
spellingShingle |
Modulation of the intestinal microbiota of broilers supplemented with monensin or functional oils in response to challenge by Eimeria spp. Vieira, Alexandre Maciel Nutricao animal Suplemento alimentar Monensina Frango de corte Flora microbiana |
title_short |
Modulation of the intestinal microbiota of broilers supplemented with monensin or functional oils in response to challenge by Eimeria spp. |
title_full |
Modulation of the intestinal microbiota of broilers supplemented with monensin or functional oils in response to challenge by Eimeria spp. |
title_fullStr |
Modulation of the intestinal microbiota of broilers supplemented with monensin or functional oils in response to challenge by Eimeria spp. |
title_full_unstemmed |
Modulation of the intestinal microbiota of broilers supplemented with monensin or functional oils in response to challenge by Eimeria spp. |
title_sort |
Modulation of the intestinal microbiota of broilers supplemented with monensin or functional oils in response to challenge by Eimeria spp. |
author |
Vieira, Alexandre Maciel |
author_facet |
Vieira, Alexandre Maciel Soratto, Tatiany Aparecida Teixeira Cardinal, Kátia Maria Wagner, Glauber Hauptli, Lucélia Lima, André Luis Ferreira Dahlke, Fabiano Peres Netto, Diego Moraes, Priscila de Oliveira Ribeiro, Andrea Machado Leal |
author_role |
author |
author2 |
Soratto, Tatiany Aparecida Teixeira Cardinal, Kátia Maria Wagner, Glauber Hauptli, Lucélia Lima, André Luis Ferreira Dahlke, Fabiano Peres Netto, Diego Moraes, Priscila de Oliveira Ribeiro, Andrea Machado Leal |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Vieira, Alexandre Maciel Soratto, Tatiany Aparecida Teixeira Cardinal, Kátia Maria Wagner, Glauber Hauptli, Lucélia Lima, André Luis Ferreira Dahlke, Fabiano Peres Netto, Diego Moraes, Priscila de Oliveira Ribeiro, Andrea Machado Leal |
dc.subject.por.fl_str_mv |
Nutricao animal Suplemento alimentar Monensina Frango de corte Flora microbiana |
topic |
Nutricao animal Suplemento alimentar Monensina Frango de corte Flora microbiana |
description |
The objective of this study was to evaluate the effect of supplementation with 100ppm sodium monensin or 0.15% of a blend of functional oils (cashew nut oil + castor oil) on the intestinal microbiota of broilers challenged with three different Eimeria spp. The challenge was accomplished by inoculating broiler chicks with sporulated oocysts of Eimeria tenella, Eimeria acervulina, and Eimeria maxima via oral gavage. A total of 864, day-old male broiler chicks (Cobb) were randomly assigned to six treatments (eight pens/treatment; 18 broilers/ pen) in a 3 × 2 factorial arrangement, composed of three additives (control, monensin or blend), with or without Eimeria challenge. Intestinal contents was collected at 28 days of age for microbiota analysis by sequencing 16s rRNA in V3 and V4 regions using the Illumina MiSeq platform. Taxonomy was assigned through the SILVA database version 132, using the QIIME 2 software version 2019.1. No treatment effects (p > 0.05) were observed in the microbial richness at the family level estimated by Chao1 and the biodiversity assessed by Simpson’s index, except for Shannon’s index (p < 0.05). The intestinal microbiota was domi- nated by members of the order Clostridiales and Lactobacillales, followed by the families Ruminococcaceae, Bacteroidaceae, and Lactobacillaceae, regardless of treatment. When the controls were compared, in the challenged control group there was an increase in Erysi- pelotrichaceae, Lactobacillaceae, Bacteroidaceae, Streptococcaceae, and Peptostrepto- coccaceae, and a decrease in Ruminococcaceae. Similar results were found for a challenged group that received monensin, while the blend partially mitigated this variation. Therefore, the blend alleviated the impact of coccidiosis challenge on the microbiome of broilers compared to monensin. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020 |
dc.date.accessioned.fl_str_mv |
2023-03-18T03:32:47Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/256021 |
dc.identifier.issn.pt_BR.fl_str_mv |
1932-6203 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001164100 |
identifier_str_mv |
1932-6203 001164100 |
url |
http://hdl.handle.net/10183/256021 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
PLoS ONE. San Francisco. Vol. 15 no. 8 (Aug. 2020), e0237118, 15 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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