Genome-wide analysis of the Glycerol-3-Phosphate Acyltransferase (GPAT) gene family reveals the evolution and diversification of plant GPATs
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/183954 |
Resumo: | sn-Glycerol-3-phosphate 1-O-acyltransferase (GPAT) is an important enzyme that catalyzes the transfer of an acyl group from acyl-CoA or acyl-ACP to the sn-1 or sn-2 position of sn-glycerol-3-phosphate (G3P) to generate lysophosphatidic acids (LPAs). The functional studies of GPAT in plants demonstrated its importance in controlling storage and membrane lipid. Identifying genes encoding GPAT in a variety of plant species is crucial to understand their involvement in different metabolic pathways and physiological functions. Here, we performed genome-wide and evolutionary analyses of GPATs in plants. GPAT genes were identified in all algae and plants studied. The phylogenetic analysis showed that these genes group into three main clades. While clades I (GPAT9) and II (soluble GPAT) include GPATs from algae and plants, clade III (GPAT1-8) includes GPATs specific from plants that are involved in the biosynthesis of cutin or suberin. Gene organization and the expression pattern of GPATs in plants corroborate with clade formation in the phylogeny, suggesting that the evolutionary patterns is reflected in their functionality. Overall, our results provide important insights into the evolution of the plant GPATs and allowed us to explore the evolutionary mechanism underlying the functional diversification among these genes. |
id |
UFRGS-2_8d628e7ba11815ca298f7fbb8fe41905 |
---|---|
oai_identifier_str |
oai:www.lume.ufrgs.br:10183/183954 |
network_acronym_str |
UFRGS-2 |
network_name_str |
Repositório Institucional da UFRGS |
repository_id_str |
|
spelling |
Waschburger, Edgar LuisKulcheski, Franceli RodriguesVeto, Nicole MoreiraMargis, RogerioMargis-Pinheiro, MárciaZolet, Andreia Carina Turchetto2018-10-24T02:44:03Z20181415-4757http://hdl.handle.net/10183/183954001073642sn-Glycerol-3-phosphate 1-O-acyltransferase (GPAT) is an important enzyme that catalyzes the transfer of an acyl group from acyl-CoA or acyl-ACP to the sn-1 or sn-2 position of sn-glycerol-3-phosphate (G3P) to generate lysophosphatidic acids (LPAs). The functional studies of GPAT in plants demonstrated its importance in controlling storage and membrane lipid. Identifying genes encoding GPAT in a variety of plant species is crucial to understand their involvement in different metabolic pathways and physiological functions. Here, we performed genome-wide and evolutionary analyses of GPATs in plants. GPAT genes were identified in all algae and plants studied. The phylogenetic analysis showed that these genes group into three main clades. While clades I (GPAT9) and II (soluble GPAT) include GPATs from algae and plants, clade III (GPAT1-8) includes GPATs specific from plants that are involved in the biosynthesis of cutin or suberin. Gene organization and the expression pattern of GPATs in plants corroborate with clade formation in the phylogeny, suggesting that the evolutionary patterns is reflected in their functionality. Overall, our results provide important insights into the evolution of the plant GPATs and allowed us to explore the evolutionary mechanism underlying the functional diversification among these genes.application/pdfengGenetics and molecular biology. Ribeirão Preto, SP. Vol. 41, no. 1 suppl (mar. 2018), p. 353-370.LipídiosFilogeniaGlicerolGenética vegetalPlant lipidsGPAT enzymesPhylogenyEvolutionGene expressionGenome-wide analysis of the Glycerol-3-Phosphate Acyltransferase (GPAT) gene family reveals the evolution and diversification of plant GPATsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001073642.pdfTexto completo (inglês)application/pdf6457994http://www.lume.ufrgs.br/bitstream/10183/183954/1/001073642.pdfbbfe49a66602b1d7fef048f1bb77db8aMD51TEXT001073642.pdf.txt001073642.pdf.txtExtracted Texttext/plain58837http://www.lume.ufrgs.br/bitstream/10183/183954/2/001073642.pdf.txt6e6f4c67c7188ea0f2051ac4e99a4d30MD52THUMBNAIL001073642.pdf.jpg001073642.pdf.jpgGenerated Thumbnailimage/jpeg1912http://www.lume.ufrgs.br/bitstream/10183/183954/3/001073642.pdf.jpg47e4dce7ff22d2d0616441765f3b9dfdMD5310183/1839542019-08-21 02:31:01.532047oai:www.lume.ufrgs.br:10183/183954Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-08-21T05:31:01Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Genome-wide analysis of the Glycerol-3-Phosphate Acyltransferase (GPAT) gene family reveals the evolution and diversification of plant GPATs |
title |
Genome-wide analysis of the Glycerol-3-Phosphate Acyltransferase (GPAT) gene family reveals the evolution and diversification of plant GPATs |
spellingShingle |
Genome-wide analysis of the Glycerol-3-Phosphate Acyltransferase (GPAT) gene family reveals the evolution and diversification of plant GPATs Waschburger, Edgar Luis Lipídios Filogenia Glicerol Genética vegetal Plant lipids GPAT enzymes Phylogeny Evolution Gene expression |
title_short |
Genome-wide analysis of the Glycerol-3-Phosphate Acyltransferase (GPAT) gene family reveals the evolution and diversification of plant GPATs |
title_full |
Genome-wide analysis of the Glycerol-3-Phosphate Acyltransferase (GPAT) gene family reveals the evolution and diversification of plant GPATs |
title_fullStr |
Genome-wide analysis of the Glycerol-3-Phosphate Acyltransferase (GPAT) gene family reveals the evolution and diversification of plant GPATs |
title_full_unstemmed |
Genome-wide analysis of the Glycerol-3-Phosphate Acyltransferase (GPAT) gene family reveals the evolution and diversification of plant GPATs |
title_sort |
Genome-wide analysis of the Glycerol-3-Phosphate Acyltransferase (GPAT) gene family reveals the evolution and diversification of plant GPATs |
author |
Waschburger, Edgar Luis |
author_facet |
Waschburger, Edgar Luis Kulcheski, Franceli Rodrigues Veto, Nicole Moreira Margis, Rogerio Margis-Pinheiro, Márcia Zolet, Andreia Carina Turchetto |
author_role |
author |
author2 |
Kulcheski, Franceli Rodrigues Veto, Nicole Moreira Margis, Rogerio Margis-Pinheiro, Márcia Zolet, Andreia Carina Turchetto |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Waschburger, Edgar Luis Kulcheski, Franceli Rodrigues Veto, Nicole Moreira Margis, Rogerio Margis-Pinheiro, Márcia Zolet, Andreia Carina Turchetto |
dc.subject.por.fl_str_mv |
Lipídios Filogenia Glicerol Genética vegetal |
topic |
Lipídios Filogenia Glicerol Genética vegetal Plant lipids GPAT enzymes Phylogeny Evolution Gene expression |
dc.subject.eng.fl_str_mv |
Plant lipids GPAT enzymes Phylogeny Evolution Gene expression |
description |
sn-Glycerol-3-phosphate 1-O-acyltransferase (GPAT) is an important enzyme that catalyzes the transfer of an acyl group from acyl-CoA or acyl-ACP to the sn-1 or sn-2 position of sn-glycerol-3-phosphate (G3P) to generate lysophosphatidic acids (LPAs). The functional studies of GPAT in plants demonstrated its importance in controlling storage and membrane lipid. Identifying genes encoding GPAT in a variety of plant species is crucial to understand their involvement in different metabolic pathways and physiological functions. Here, we performed genome-wide and evolutionary analyses of GPATs in plants. GPAT genes were identified in all algae and plants studied. The phylogenetic analysis showed that these genes group into three main clades. While clades I (GPAT9) and II (soluble GPAT) include GPATs from algae and plants, clade III (GPAT1-8) includes GPATs specific from plants that are involved in the biosynthesis of cutin or suberin. Gene organization and the expression pattern of GPATs in plants corroborate with clade formation in the phylogeny, suggesting that the evolutionary patterns is reflected in their functionality. Overall, our results provide important insights into the evolution of the plant GPATs and allowed us to explore the evolutionary mechanism underlying the functional diversification among these genes. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-10-24T02:44:03Z |
dc.date.issued.fl_str_mv |
2018 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/183954 |
dc.identifier.issn.pt_BR.fl_str_mv |
1415-4757 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001073642 |
identifier_str_mv |
1415-4757 001073642 |
url |
http://hdl.handle.net/10183/183954 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Genetics and molecular biology. Ribeirão Preto, SP. Vol. 41, no. 1 suppl (mar. 2018), p. 353-370. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Repositório Institucional da UFRGS |
collection |
Repositório Institucional da UFRGS |
bitstream.url.fl_str_mv |
http://www.lume.ufrgs.br/bitstream/10183/183954/1/001073642.pdf http://www.lume.ufrgs.br/bitstream/10183/183954/2/001073642.pdf.txt http://www.lume.ufrgs.br/bitstream/10183/183954/3/001073642.pdf.jpg |
bitstream.checksum.fl_str_mv |
bbfe49a66602b1d7fef048f1bb77db8a 6e6f4c67c7188ea0f2051ac4e99a4d30 47e4dce7ff22d2d0616441765f3b9dfd |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
|
_version_ |
1801224953838698496 |