In vitro method to evaluate virus competition between BVDV-1 and BVDV-2 strains using the PrimeFlow RNA assay
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/200982 |
Resumo: | Bovine viral diarrhea viruses (BVDV), segregated in BVDV-1 and BVDV-2 species, lead to substantial economic losses to the cattle industry worldwide. It has been hypothesized that there could be differences in level of replication, pathogenesis and tissue tropism between BVDV-1 and BVDV-2 strains. Thus, this study developed an in vitro method to evaluate virus competition between BVDV-1 and BVDV-2 strains. To this end the competitive dynamics of BVDV-1a, BVDV-1b, and BVDV-2a strains in cell cultures was evaluated by a PrimeFlow RNA assay. Similar results were observed in this study, as was observed in an earlier in vivo transmission study. Competitive exclusion was observed as the BVDV-2a strains dominated and excluded the BVDV-1a and BVDV-1b strains. The in vitro model developed can be used to identify viral variations that result in differences in frequency of subgenotypes detected in the field, vaccine failure, pathogenesis, and strain dependent variation in immune responses. |
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Silveira, SimoneFalkenberg, Shollie M.Dassanayake, Rohana P.Walz, Paul H.Ridpath, J.F.Canal, Cláudio WageckNeill, John D.2019-10-25T03:46:29Z20190042-6822http://hdl.handle.net/10183/200982001103488Bovine viral diarrhea viruses (BVDV), segregated in BVDV-1 and BVDV-2 species, lead to substantial economic losses to the cattle industry worldwide. It has been hypothesized that there could be differences in level of replication, pathogenesis and tissue tropism between BVDV-1 and BVDV-2 strains. Thus, this study developed an in vitro method to evaluate virus competition between BVDV-1 and BVDV-2 strains. To this end the competitive dynamics of BVDV-1a, BVDV-1b, and BVDV-2a strains in cell cultures was evaluated by a PrimeFlow RNA assay. Similar results were observed in this study, as was observed in an earlier in vivo transmission study. Competitive exclusion was observed as the BVDV-2a strains dominated and excluded the BVDV-1a and BVDV-1b strains. The in vitro model developed can be used to identify viral variations that result in differences in frequency of subgenotypes detected in the field, vaccine failure, pathogenesis, and strain dependent variation in immune responses.application/pdfengVirology. Vol. 536 (Oct. 2019), p. 101-109Técnicas In VitroCompetição biológicaVírus da diarréia viral bovina tipo 1Vírus da diarréia viral bovina tipo 2Citometria de fluxoIn vitro method to evaluate virus competition between BVDV-1 and BVDV-2 strains using the PrimeFlow RNA assayEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001103488.pdf.txt001103488.pdf.txtExtracted Texttext/plain54866http://www.lume.ufrgs.br/bitstream/10183/200982/2/001103488.pdf.txtc14b209f1560cb7657f0381fdd445b5dMD52ORIGINAL001103488.pdfTexto completo (inglês)application/pdf524627http://www.lume.ufrgs.br/bitstream/10183/200982/1/001103488.pdfdb8676c3ac5d871141eb02ed71ee2f6fMD5110183/2009822019-10-26 03:49:51.77626oai:www.lume.ufrgs.br:10183/200982Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-10-26T06:49:51Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
In vitro method to evaluate virus competition between BVDV-1 and BVDV-2 strains using the PrimeFlow RNA assay |
title |
In vitro method to evaluate virus competition between BVDV-1 and BVDV-2 strains using the PrimeFlow RNA assay |
spellingShingle |
In vitro method to evaluate virus competition between BVDV-1 and BVDV-2 strains using the PrimeFlow RNA assay Silveira, Simone Técnicas In Vitro Competição biológica Vírus da diarréia viral bovina tipo 1 Vírus da diarréia viral bovina tipo 2 Citometria de fluxo |
title_short |
In vitro method to evaluate virus competition between BVDV-1 and BVDV-2 strains using the PrimeFlow RNA assay |
title_full |
In vitro method to evaluate virus competition between BVDV-1 and BVDV-2 strains using the PrimeFlow RNA assay |
title_fullStr |
In vitro method to evaluate virus competition between BVDV-1 and BVDV-2 strains using the PrimeFlow RNA assay |
title_full_unstemmed |
In vitro method to evaluate virus competition between BVDV-1 and BVDV-2 strains using the PrimeFlow RNA assay |
title_sort |
In vitro method to evaluate virus competition between BVDV-1 and BVDV-2 strains using the PrimeFlow RNA assay |
author |
Silveira, Simone |
author_facet |
Silveira, Simone Falkenberg, Shollie M. Dassanayake, Rohana P. Walz, Paul H. Ridpath, J.F. Canal, Cláudio Wageck Neill, John D. |
author_role |
author |
author2 |
Falkenberg, Shollie M. Dassanayake, Rohana P. Walz, Paul H. Ridpath, J.F. Canal, Cláudio Wageck Neill, John D. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Silveira, Simone Falkenberg, Shollie M. Dassanayake, Rohana P. Walz, Paul H. Ridpath, J.F. Canal, Cláudio Wageck Neill, John D. |
dc.subject.por.fl_str_mv |
Técnicas In Vitro Competição biológica Vírus da diarréia viral bovina tipo 1 Vírus da diarréia viral bovina tipo 2 Citometria de fluxo |
topic |
Técnicas In Vitro Competição biológica Vírus da diarréia viral bovina tipo 1 Vírus da diarréia viral bovina tipo 2 Citometria de fluxo |
description |
Bovine viral diarrhea viruses (BVDV), segregated in BVDV-1 and BVDV-2 species, lead to substantial economic losses to the cattle industry worldwide. It has been hypothesized that there could be differences in level of replication, pathogenesis and tissue tropism between BVDV-1 and BVDV-2 strains. Thus, this study developed an in vitro method to evaluate virus competition between BVDV-1 and BVDV-2 strains. To this end the competitive dynamics of BVDV-1a, BVDV-1b, and BVDV-2a strains in cell cultures was evaluated by a PrimeFlow RNA assay. Similar results were observed in this study, as was observed in an earlier in vivo transmission study. Competitive exclusion was observed as the BVDV-2a strains dominated and excluded the BVDV-1a and BVDV-1b strains. The in vitro model developed can be used to identify viral variations that result in differences in frequency of subgenotypes detected in the field, vaccine failure, pathogenesis, and strain dependent variation in immune responses. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-10-25T03:46:29Z |
dc.date.issued.fl_str_mv |
2019 |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/200982 |
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0042-6822 |
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001103488 |
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http://hdl.handle.net/10183/200982 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Virology. Vol. 536 (Oct. 2019), p. 101-109 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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