First report of free-living amoebae in watercourses in southern Brazil : molecular diagnosis and phylogenetic analysis of Vermamoeba vermiformis, Naegleria gruberi, and Acanthamoeba spp.

Detalhes bibliográficos
Autor(a) principal: Marinho, Brenda Teixeira Scardini
Data de Publicação: 2023
Outros Autores: Santos, Denise Leal dos, Santos, Daniel Leal dos, Rott, Marilise Brittes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/265136
Resumo: Free-living amoebae (FLA) are protozoa dispersed in different environments and are responsible for different infections caused to humans and other animals. Microorganisms such as Acanthamoeba spp., Vermamoeba sp., and Naegleria sp. are associated with diseases that affect the central nervous system, in addition to skin infections and keratitis, as occurs in the genus Acanthamoeba and with Vermamoeba vermiformis. Due to the concerns of these FLA in anthropogenic aquatic environments, this work aimed to identify these microorganisms present in waters of Porto Alegre, Brazil. One litre sample was collected in two watercourses during the summer of 2022 and inoculated onto non-nutrient agar plates containing heat-inactivated Escherichia coli. Polymerase chain reaction results indicated the presence of FLA of the genera Acanthamoeba, Vermamoeba, and Naegleria in the study areas. Genetic sequencing indicated the presence of V. vermiformis and Naegleria gruberi. These aquatic and anthropogenic environments can serve as a means of spread and contamination by FLA, which gives valuable information on public health in the city.
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spelling Marinho, Brenda Teixeira ScardiniSantos, Denise Leal dosSantos, Daniel Leal dosRott, Marilise Brittes2023-09-23T03:37:32Z20231996-7829http://hdl.handle.net/10183/265136001174880Free-living amoebae (FLA) are protozoa dispersed in different environments and are responsible for different infections caused to humans and other animals. Microorganisms such as Acanthamoeba spp., Vermamoeba sp., and Naegleria sp. are associated with diseases that affect the central nervous system, in addition to skin infections and keratitis, as occurs in the genus Acanthamoeba and with Vermamoeba vermiformis. Due to the concerns of these FLA in anthropogenic aquatic environments, this work aimed to identify these microorganisms present in waters of Porto Alegre, Brazil. One litre sample was collected in two watercourses during the summer of 2022 and inoculated onto non-nutrient agar plates containing heat-inactivated Escherichia coli. Polymerase chain reaction results indicated the presence of FLA of the genera Acanthamoeba, Vermamoeba, and Naegleria in the study areas. Genetic sequencing indicated the presence of V. vermiformis and Naegleria gruberi. These aquatic and anthropogenic environments can serve as a means of spread and contamination by FLA, which gives valuable information on public health in the city.application/pdfengJournal of water and health. London. Vol. 21, no. 7 (July 2023), p. 972-980Infecções protozoárias do sistema nervoso centralAmoebaMeio ambiente e saúde públicaGuaíba, Lago (RS)Acanthamoeba spp.Dilúvio streamFree-living amoebaeGuaíba lakeNaegleria gruberiVermamoeba vermiformisFirst report of free-living amoebae in watercourses in southern Brazil : molecular diagnosis and phylogenetic analysis of Vermamoeba vermiformis, Naegleria gruberi, and Acanthamoeba spp.Estrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001174880.pdf.txt001174880.pdf.txtExtracted Texttext/plain26546http://www.lume.ufrgs.br/bitstream/10183/265136/2/001174880.pdf.txt397b70c97f66984c886c18dd55fd00d2MD52ORIGINAL001174880.pdfTexto completo (inglês)application/pdf696824http://www.lume.ufrgs.br/bitstream/10183/265136/1/001174880.pdf80ee7547f4aba0a33aeb6dde9f9527c4MD5110183/2651362023-09-24 03:39:33.184699oai:www.lume.ufrgs.br:10183/265136Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-09-24T06:39:33Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv First report of free-living amoebae in watercourses in southern Brazil : molecular diagnosis and phylogenetic analysis of Vermamoeba vermiformis, Naegleria gruberi, and Acanthamoeba spp.
title First report of free-living amoebae in watercourses in southern Brazil : molecular diagnosis and phylogenetic analysis of Vermamoeba vermiformis, Naegleria gruberi, and Acanthamoeba spp.
spellingShingle First report of free-living amoebae in watercourses in southern Brazil : molecular diagnosis and phylogenetic analysis of Vermamoeba vermiformis, Naegleria gruberi, and Acanthamoeba spp.
Marinho, Brenda Teixeira Scardini
Infecções protozoárias do sistema nervoso central
Amoeba
Meio ambiente e saúde pública
Guaíba, Lago (RS)
Acanthamoeba spp.
Dilúvio stream
Free-living amoebae
Guaíba lake
Naegleria gruberi
Vermamoeba vermiformis
title_short First report of free-living amoebae in watercourses in southern Brazil : molecular diagnosis and phylogenetic analysis of Vermamoeba vermiformis, Naegleria gruberi, and Acanthamoeba spp.
title_full First report of free-living amoebae in watercourses in southern Brazil : molecular diagnosis and phylogenetic analysis of Vermamoeba vermiformis, Naegleria gruberi, and Acanthamoeba spp.
title_fullStr First report of free-living amoebae in watercourses in southern Brazil : molecular diagnosis and phylogenetic analysis of Vermamoeba vermiformis, Naegleria gruberi, and Acanthamoeba spp.
title_full_unstemmed First report of free-living amoebae in watercourses in southern Brazil : molecular diagnosis and phylogenetic analysis of Vermamoeba vermiformis, Naegleria gruberi, and Acanthamoeba spp.
title_sort First report of free-living amoebae in watercourses in southern Brazil : molecular diagnosis and phylogenetic analysis of Vermamoeba vermiformis, Naegleria gruberi, and Acanthamoeba spp.
author Marinho, Brenda Teixeira Scardini
author_facet Marinho, Brenda Teixeira Scardini
Santos, Denise Leal dos
Santos, Daniel Leal dos
Rott, Marilise Brittes
author_role author
author2 Santos, Denise Leal dos
Santos, Daniel Leal dos
Rott, Marilise Brittes
author2_role author
author
author
dc.contributor.author.fl_str_mv Marinho, Brenda Teixeira Scardini
Santos, Denise Leal dos
Santos, Daniel Leal dos
Rott, Marilise Brittes
dc.subject.por.fl_str_mv Infecções protozoárias do sistema nervoso central
Amoeba
Meio ambiente e saúde pública
Guaíba, Lago (RS)
topic Infecções protozoárias do sistema nervoso central
Amoeba
Meio ambiente e saúde pública
Guaíba, Lago (RS)
Acanthamoeba spp.
Dilúvio stream
Free-living amoebae
Guaíba lake
Naegleria gruberi
Vermamoeba vermiformis
dc.subject.eng.fl_str_mv Acanthamoeba spp.
Dilúvio stream
Free-living amoebae
Guaíba lake
Naegleria gruberi
Vermamoeba vermiformis
description Free-living amoebae (FLA) are protozoa dispersed in different environments and are responsible for different infections caused to humans and other animals. Microorganisms such as Acanthamoeba spp., Vermamoeba sp., and Naegleria sp. are associated with diseases that affect the central nervous system, in addition to skin infections and keratitis, as occurs in the genus Acanthamoeba and with Vermamoeba vermiformis. Due to the concerns of these FLA in anthropogenic aquatic environments, this work aimed to identify these microorganisms present in waters of Porto Alegre, Brazil. One litre sample was collected in two watercourses during the summer of 2022 and inoculated onto non-nutrient agar plates containing heat-inactivated Escherichia coli. Polymerase chain reaction results indicated the presence of FLA of the genera Acanthamoeba, Vermamoeba, and Naegleria in the study areas. Genetic sequencing indicated the presence of V. vermiformis and Naegleria gruberi. These aquatic and anthropogenic environments can serve as a means of spread and contamination by FLA, which gives valuable information on public health in the city.
publishDate 2023
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dc.identifier.issn.pt_BR.fl_str_mv 1996-7829
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dc.relation.ispartof.pt_BR.fl_str_mv Journal of water and health. London. Vol. 21, no. 7 (July 2023), p. 972-980
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