First report of free-living amoebae in watercourses in southern Brazil : molecular diagnosis and phylogenetic analysis of Vermamoeba vermiformis, Naegleria gruberi, and Acanthamoeba spp.
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/265136 |
Resumo: | Free-living amoebae (FLA) are protozoa dispersed in different environments and are responsible for different infections caused to humans and other animals. Microorganisms such as Acanthamoeba spp., Vermamoeba sp., and Naegleria sp. are associated with diseases that affect the central nervous system, in addition to skin infections and keratitis, as occurs in the genus Acanthamoeba and with Vermamoeba vermiformis. Due to the concerns of these FLA in anthropogenic aquatic environments, this work aimed to identify these microorganisms present in waters of Porto Alegre, Brazil. One litre sample was collected in two watercourses during the summer of 2022 and inoculated onto non-nutrient agar plates containing heat-inactivated Escherichia coli. Polymerase chain reaction results indicated the presence of FLA of the genera Acanthamoeba, Vermamoeba, and Naegleria in the study areas. Genetic sequencing indicated the presence of V. vermiformis and Naegleria gruberi. These aquatic and anthropogenic environments can serve as a means of spread and contamination by FLA, which gives valuable information on public health in the city. |
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Marinho, Brenda Teixeira ScardiniSantos, Denise Leal dosSantos, Daniel Leal dosRott, Marilise Brittes2023-09-23T03:37:32Z20231996-7829http://hdl.handle.net/10183/265136001174880Free-living amoebae (FLA) are protozoa dispersed in different environments and are responsible for different infections caused to humans and other animals. Microorganisms such as Acanthamoeba spp., Vermamoeba sp., and Naegleria sp. are associated with diseases that affect the central nervous system, in addition to skin infections and keratitis, as occurs in the genus Acanthamoeba and with Vermamoeba vermiformis. Due to the concerns of these FLA in anthropogenic aquatic environments, this work aimed to identify these microorganisms present in waters of Porto Alegre, Brazil. One litre sample was collected in two watercourses during the summer of 2022 and inoculated onto non-nutrient agar plates containing heat-inactivated Escherichia coli. Polymerase chain reaction results indicated the presence of FLA of the genera Acanthamoeba, Vermamoeba, and Naegleria in the study areas. Genetic sequencing indicated the presence of V. vermiformis and Naegleria gruberi. These aquatic and anthropogenic environments can serve as a means of spread and contamination by FLA, which gives valuable information on public health in the city.application/pdfengJournal of water and health. London. Vol. 21, no. 7 (July 2023), p. 972-980Infecções protozoárias do sistema nervoso centralAmoebaMeio ambiente e saúde públicaGuaíba, Lago (RS)Acanthamoeba spp.Dilúvio streamFree-living amoebaeGuaíba lakeNaegleria gruberiVermamoeba vermiformisFirst report of free-living amoebae in watercourses in southern Brazil : molecular diagnosis and phylogenetic analysis of Vermamoeba vermiformis, Naegleria gruberi, and Acanthamoeba spp.Estrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001174880.pdf.txt001174880.pdf.txtExtracted Texttext/plain26546http://www.lume.ufrgs.br/bitstream/10183/265136/2/001174880.pdf.txt397b70c97f66984c886c18dd55fd00d2MD52ORIGINAL001174880.pdfTexto completo (inglês)application/pdf696824http://www.lume.ufrgs.br/bitstream/10183/265136/1/001174880.pdf80ee7547f4aba0a33aeb6dde9f9527c4MD5110183/2651362023-09-24 03:39:33.184699oai:www.lume.ufrgs.br:10183/265136Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-09-24T06:39:33Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
First report of free-living amoebae in watercourses in southern Brazil : molecular diagnosis and phylogenetic analysis of Vermamoeba vermiformis, Naegleria gruberi, and Acanthamoeba spp. |
title |
First report of free-living amoebae in watercourses in southern Brazil : molecular diagnosis and phylogenetic analysis of Vermamoeba vermiformis, Naegleria gruberi, and Acanthamoeba spp. |
spellingShingle |
First report of free-living amoebae in watercourses in southern Brazil : molecular diagnosis and phylogenetic analysis of Vermamoeba vermiformis, Naegleria gruberi, and Acanthamoeba spp. Marinho, Brenda Teixeira Scardini Infecções protozoárias do sistema nervoso central Amoeba Meio ambiente e saúde pública Guaíba, Lago (RS) Acanthamoeba spp. Dilúvio stream Free-living amoebae Guaíba lake Naegleria gruberi Vermamoeba vermiformis |
title_short |
First report of free-living amoebae in watercourses in southern Brazil : molecular diagnosis and phylogenetic analysis of Vermamoeba vermiformis, Naegleria gruberi, and Acanthamoeba spp. |
title_full |
First report of free-living amoebae in watercourses in southern Brazil : molecular diagnosis and phylogenetic analysis of Vermamoeba vermiformis, Naegleria gruberi, and Acanthamoeba spp. |
title_fullStr |
First report of free-living amoebae in watercourses in southern Brazil : molecular diagnosis and phylogenetic analysis of Vermamoeba vermiformis, Naegleria gruberi, and Acanthamoeba spp. |
title_full_unstemmed |
First report of free-living amoebae in watercourses in southern Brazil : molecular diagnosis and phylogenetic analysis of Vermamoeba vermiformis, Naegleria gruberi, and Acanthamoeba spp. |
title_sort |
First report of free-living amoebae in watercourses in southern Brazil : molecular diagnosis and phylogenetic analysis of Vermamoeba vermiformis, Naegleria gruberi, and Acanthamoeba spp. |
author |
Marinho, Brenda Teixeira Scardini |
author_facet |
Marinho, Brenda Teixeira Scardini Santos, Denise Leal dos Santos, Daniel Leal dos Rott, Marilise Brittes |
author_role |
author |
author2 |
Santos, Denise Leal dos Santos, Daniel Leal dos Rott, Marilise Brittes |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Marinho, Brenda Teixeira Scardini Santos, Denise Leal dos Santos, Daniel Leal dos Rott, Marilise Brittes |
dc.subject.por.fl_str_mv |
Infecções protozoárias do sistema nervoso central Amoeba Meio ambiente e saúde pública Guaíba, Lago (RS) |
topic |
Infecções protozoárias do sistema nervoso central Amoeba Meio ambiente e saúde pública Guaíba, Lago (RS) Acanthamoeba spp. Dilúvio stream Free-living amoebae Guaíba lake Naegleria gruberi Vermamoeba vermiformis |
dc.subject.eng.fl_str_mv |
Acanthamoeba spp. Dilúvio stream Free-living amoebae Guaíba lake Naegleria gruberi Vermamoeba vermiformis |
description |
Free-living amoebae (FLA) are protozoa dispersed in different environments and are responsible for different infections caused to humans and other animals. Microorganisms such as Acanthamoeba spp., Vermamoeba sp., and Naegleria sp. are associated with diseases that affect the central nervous system, in addition to skin infections and keratitis, as occurs in the genus Acanthamoeba and with Vermamoeba vermiformis. Due to the concerns of these FLA in anthropogenic aquatic environments, this work aimed to identify these microorganisms present in waters of Porto Alegre, Brazil. One litre sample was collected in two watercourses during the summer of 2022 and inoculated onto non-nutrient agar plates containing heat-inactivated Escherichia coli. Polymerase chain reaction results indicated the presence of FLA of the genera Acanthamoeba, Vermamoeba, and Naegleria in the study areas. Genetic sequencing indicated the presence of V. vermiformis and Naegleria gruberi. These aquatic and anthropogenic environments can serve as a means of spread and contamination by FLA, which gives valuable information on public health in the city. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-09-23T03:37:32Z |
dc.date.issued.fl_str_mv |
2023 |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/265136 |
dc.identifier.issn.pt_BR.fl_str_mv |
1996-7829 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001174880 |
identifier_str_mv |
1996-7829 001174880 |
url |
http://hdl.handle.net/10183/265136 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Journal of water and health. London. Vol. 21, no. 7 (July 2023), p. 972-980 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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