Low inflammatory stimulus increases D2 activity and modulates thyroid hormone metabolism during myogenesis in vitro
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/259637 |
Resumo: | Thyroid hormone (TH) signaling controls muscle progenitor cells differentiation. However, inflammation can alter muscle TH signaling by modulating the expression of TH transporters (Slc16a2), receptors (Thra1), and deiodinase enzymes (Dio2 and Dio3). Thus, a proinflammatory environment could affect myogenesis. The role of a low-grade inflammatory milieu in TH signaling during myogenesis needs further investigation. Herein, we aimed to study the impact of the bacterial lipopolysaccharide (LPS)-induced inflammatory stimulus on the TH signaling during myogenesis. C2C12 myoblasts differentiation was induced without (CTR) or with 10 ng/mL LPS presence. The myoblasts under LPS stimulus release the proinflammatory cytokines (IL-6 and IL-1β) and chemokines (CCL2 and CXCL-1). LPS decreases Myod1 expression by 28% during the initial myogenesis, thus reducing the myogenic stimulus. At the same time, LPS reduced the expression of Dio2 by 41% but doubled the D2 enzymatic activity. The late differentiation was not affected by inflammatory milieu, which only increased the Slc16a2 gene expression by 38%. LPS altered the intracellular metabolism of TH and reduced the initial myogenic stimulus. However, it did not affect late differentiation. Increased intracellular TH activation may be the compensatory pathway involved in the recovery of myogenic differentiation under a low-grade inflammatory milieu. |
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Oliveira, Thamires Siqueira deShimabukuro, Marilia KimieMonteiro, Victoria Regina SiqueiraAndrade, Cherley Borba Vieira deBoelen, AnitaWajner, Simone MagagninMaia, Ana Luiza SilvaOrtiga-Carvalho, TaniaBloise, Flavia Fonseca2023-06-29T03:30:52Z20222218-1989http://hdl.handle.net/10183/259637001168592Thyroid hormone (TH) signaling controls muscle progenitor cells differentiation. However, inflammation can alter muscle TH signaling by modulating the expression of TH transporters (Slc16a2), receptors (Thra1), and deiodinase enzymes (Dio2 and Dio3). Thus, a proinflammatory environment could affect myogenesis. The role of a low-grade inflammatory milieu in TH signaling during myogenesis needs further investigation. Herein, we aimed to study the impact of the bacterial lipopolysaccharide (LPS)-induced inflammatory stimulus on the TH signaling during myogenesis. C2C12 myoblasts differentiation was induced without (CTR) or with 10 ng/mL LPS presence. The myoblasts under LPS stimulus release the proinflammatory cytokines (IL-6 and IL-1β) and chemokines (CCL2 and CXCL-1). LPS decreases Myod1 expression by 28% during the initial myogenesis, thus reducing the myogenic stimulus. At the same time, LPS reduced the expression of Dio2 by 41% but doubled the D2 enzymatic activity. The late differentiation was not affected by inflammatory milieu, which only increased the Slc16a2 gene expression by 38%. LPS altered the intracellular metabolism of TH and reduced the initial myogenic stimulus. However, it did not affect late differentiation. Increased intracellular TH activation may be the compensatory pathway involved in the recovery of myogenic differentiation under a low-grade inflammatory milieu.application/pdfengMetabolites. Basel. Vol. 12, no. 5 (2022), 416, 10 p.LipopolissacarídeosIodeto peroxidaseLinhagem celularMioblastos esqueléticosCélulas satélites de músculo esqueléticoTri-IodotironinaC2C12Bacterial lipopolysaccharideDeiodinaseInflammationMyoblastMyogenic differentiationTriiodothyronineLow inflammatory stimulus increases D2 activity and modulates thyroid hormone metabolism during myogenesis in vitroEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001168592.pdf.txt001168592.pdf.txtExtracted Texttext/plain46911http://www.lume.ufrgs.br/bitstream/10183/259637/2/001168592.pdf.txt150832d00ab0b9777009a7c52c88451aMD52ORIGINAL001168592.pdfTexto completo (inglês)application/pdf858382http://www.lume.ufrgs.br/bitstream/10183/259637/1/001168592.pdfc542eb3995cd9d3d1e8e70eb0baa9e9cMD5110183/2596372023-06-30 03:33:43.105195oai:www.lume.ufrgs.br:10183/259637Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-06-30T06:33:43Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Low inflammatory stimulus increases D2 activity and modulates thyroid hormone metabolism during myogenesis in vitro |
title |
Low inflammatory stimulus increases D2 activity and modulates thyroid hormone metabolism during myogenesis in vitro |
spellingShingle |
Low inflammatory stimulus increases D2 activity and modulates thyroid hormone metabolism during myogenesis in vitro Oliveira, Thamires Siqueira de Lipopolissacarídeos Iodeto peroxidase Linhagem celular Mioblastos esqueléticos Células satélites de músculo esquelético Tri-Iodotironina C2C12 Bacterial lipopolysaccharide Deiodinase Inflammation Myoblast Myogenic differentiation Triiodothyronine |
title_short |
Low inflammatory stimulus increases D2 activity and modulates thyroid hormone metabolism during myogenesis in vitro |
title_full |
Low inflammatory stimulus increases D2 activity and modulates thyroid hormone metabolism during myogenesis in vitro |
title_fullStr |
Low inflammatory stimulus increases D2 activity and modulates thyroid hormone metabolism during myogenesis in vitro |
title_full_unstemmed |
Low inflammatory stimulus increases D2 activity and modulates thyroid hormone metabolism during myogenesis in vitro |
title_sort |
Low inflammatory stimulus increases D2 activity and modulates thyroid hormone metabolism during myogenesis in vitro |
author |
Oliveira, Thamires Siqueira de |
author_facet |
Oliveira, Thamires Siqueira de Shimabukuro, Marilia Kimie Monteiro, Victoria Regina Siqueira Andrade, Cherley Borba Vieira de Boelen, Anita Wajner, Simone Magagnin Maia, Ana Luiza Silva Ortiga-Carvalho, Tania Bloise, Flavia Fonseca |
author_role |
author |
author2 |
Shimabukuro, Marilia Kimie Monteiro, Victoria Regina Siqueira Andrade, Cherley Borba Vieira de Boelen, Anita Wajner, Simone Magagnin Maia, Ana Luiza Silva Ortiga-Carvalho, Tania Bloise, Flavia Fonseca |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Thamires Siqueira de Shimabukuro, Marilia Kimie Monteiro, Victoria Regina Siqueira Andrade, Cherley Borba Vieira de Boelen, Anita Wajner, Simone Magagnin Maia, Ana Luiza Silva Ortiga-Carvalho, Tania Bloise, Flavia Fonseca |
dc.subject.por.fl_str_mv |
Lipopolissacarídeos Iodeto peroxidase Linhagem celular Mioblastos esqueléticos Células satélites de músculo esquelético Tri-Iodotironina |
topic |
Lipopolissacarídeos Iodeto peroxidase Linhagem celular Mioblastos esqueléticos Células satélites de músculo esquelético Tri-Iodotironina C2C12 Bacterial lipopolysaccharide Deiodinase Inflammation Myoblast Myogenic differentiation Triiodothyronine |
dc.subject.eng.fl_str_mv |
C2C12 Bacterial lipopolysaccharide Deiodinase Inflammation Myoblast Myogenic differentiation Triiodothyronine |
description |
Thyroid hormone (TH) signaling controls muscle progenitor cells differentiation. However, inflammation can alter muscle TH signaling by modulating the expression of TH transporters (Slc16a2), receptors (Thra1), and deiodinase enzymes (Dio2 and Dio3). Thus, a proinflammatory environment could affect myogenesis. The role of a low-grade inflammatory milieu in TH signaling during myogenesis needs further investigation. Herein, we aimed to study the impact of the bacterial lipopolysaccharide (LPS)-induced inflammatory stimulus on the TH signaling during myogenesis. C2C12 myoblasts differentiation was induced without (CTR) or with 10 ng/mL LPS presence. The myoblasts under LPS stimulus release the proinflammatory cytokines (IL-6 and IL-1β) and chemokines (CCL2 and CXCL-1). LPS decreases Myod1 expression by 28% during the initial myogenesis, thus reducing the myogenic stimulus. At the same time, LPS reduced the expression of Dio2 by 41% but doubled the D2 enzymatic activity. The late differentiation was not affected by inflammatory milieu, which only increased the Slc16a2 gene expression by 38%. LPS altered the intracellular metabolism of TH and reduced the initial myogenic stimulus. However, it did not affect late differentiation. Increased intracellular TH activation may be the compensatory pathway involved in the recovery of myogenic differentiation under a low-grade inflammatory milieu. |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022 |
dc.date.accessioned.fl_str_mv |
2023-06-29T03:30:52Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/259637 |
dc.identifier.issn.pt_BR.fl_str_mv |
2218-1989 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001168592 |
identifier_str_mv |
2218-1989 001168592 |
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http://hdl.handle.net/10183/259637 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Metabolites. Basel. Vol. 12, no. 5 (2022), 416, 10 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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