Biocontainment Techniques and Applications for Yeast Biotechnology
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/274301 |
Resumo: | Biocontainment techniques for genetically modified yeasts (GMYs) are pivotal due to the importance of these organisms for biotechnological processes and also due to the design of new yeast strains by using synthetic biology tools and technologies. Due to the large genetic modifications that many yeast strains display, it is highly desirable to avoid the leakage of GMY cells into natural environments and, consequently, the spread of synthetic genes and circuits by horizontal or vertical gene transfer mechanisms within the microorganisms. Moreover, it is also desirable to avoid patented yeast gene technologies spreading outside the production facility. In this review, the different biocontainment technologies currently available for GMYs were evaluated. Interestingly, uniplex-type biocontainment approaches (UTBAs), which rely on nutrient auxotrophies induced by gene mutation or deletion or the expression of the simple kill switches apparatus, are still the major biocontainment approaches in use with GMY. While bacteria such as Escherichia coli account for advanced biocontainment technologies based on synthetic biology and multiplex-type biocontainment approaches (MTBAs), GMYs are distant from this scenario due to many reasons. Thus, a comparison of different UTBAs and MTBAs applied for GMY and genetically engineered microorganisms (GEMs) was made, indicating the major advances of biocontainment techniques for GMYs. |
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Silva, Guilherme Eduardo Pavão daSilva, Isabela Sfalcin daBonatto, Diego2024-03-28T06:23:05Z20232311-5637http://hdl.handle.net/10183/274301001172016Biocontainment techniques for genetically modified yeasts (GMYs) are pivotal due to the importance of these organisms for biotechnological processes and also due to the design of new yeast strains by using synthetic biology tools and technologies. Due to the large genetic modifications that many yeast strains display, it is highly desirable to avoid the leakage of GMY cells into natural environments and, consequently, the spread of synthetic genes and circuits by horizontal or vertical gene transfer mechanisms within the microorganisms. Moreover, it is also desirable to avoid patented yeast gene technologies spreading outside the production facility. In this review, the different biocontainment technologies currently available for GMYs were evaluated. Interestingly, uniplex-type biocontainment approaches (UTBAs), which rely on nutrient auxotrophies induced by gene mutation or deletion or the expression of the simple kill switches apparatus, are still the major biocontainment approaches in use with GMY. While bacteria such as Escherichia coli account for advanced biocontainment technologies based on synthetic biology and multiplex-type biocontainment approaches (MTBAs), GMYs are distant from this scenario due to many reasons. Thus, a comparison of different UTBAs and MTBAs applied for GMY and genetically engineered microorganisms (GEMs) was made, indicating the major advances of biocontainment techniques for GMYs.application/pdfengFermentation. Switzerland. Vol. 9, no. 4 (Apr. 2023), e341, 16 p.Leveduras : GenéticaBiologia sintéticaAuxotrofiaBiocontainmentGenetically engineered microorganismsGenetically modified yeastsGene circuitsBiocontainment Techniques and Applications for Yeast BiotechnologyEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001172016.pdf.txt001172016.pdf.txtExtracted Texttext/plain76036http://www.lume.ufrgs.br/bitstream/10183/274301/2/001172016.pdf.txt56e19c4c253338fcb1f54d8e58bbc867MD52ORIGINAL001172016.pdfTexto completo (inglês)application/pdf757950http://www.lume.ufrgs.br/bitstream/10183/274301/1/001172016.pdfe3ae07cdf754ca1ddbedd89d3261cf00MD5110183/2743012024-03-29 06:19:03.708794oai:www.lume.ufrgs.br:10183/274301Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-03-29T09:19:03Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Biocontainment Techniques and Applications for Yeast Biotechnology |
title |
Biocontainment Techniques and Applications for Yeast Biotechnology |
spellingShingle |
Biocontainment Techniques and Applications for Yeast Biotechnology Silva, Guilherme Eduardo Pavão da Leveduras : Genética Biologia sintética Auxotrofia Biocontainment Genetically engineered microorganisms Genetically modified yeasts Gene circuits |
title_short |
Biocontainment Techniques and Applications for Yeast Biotechnology |
title_full |
Biocontainment Techniques and Applications for Yeast Biotechnology |
title_fullStr |
Biocontainment Techniques and Applications for Yeast Biotechnology |
title_full_unstemmed |
Biocontainment Techniques and Applications for Yeast Biotechnology |
title_sort |
Biocontainment Techniques and Applications for Yeast Biotechnology |
author |
Silva, Guilherme Eduardo Pavão da |
author_facet |
Silva, Guilherme Eduardo Pavão da Silva, Isabela Sfalcin da Bonatto, Diego |
author_role |
author |
author2 |
Silva, Isabela Sfalcin da Bonatto, Diego |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Silva, Guilherme Eduardo Pavão da Silva, Isabela Sfalcin da Bonatto, Diego |
dc.subject.por.fl_str_mv |
Leveduras : Genética Biologia sintética Auxotrofia |
topic |
Leveduras : Genética Biologia sintética Auxotrofia Biocontainment Genetically engineered microorganisms Genetically modified yeasts Gene circuits |
dc.subject.eng.fl_str_mv |
Biocontainment Genetically engineered microorganisms Genetically modified yeasts Gene circuits |
description |
Biocontainment techniques for genetically modified yeasts (GMYs) are pivotal due to the importance of these organisms for biotechnological processes and also due to the design of new yeast strains by using synthetic biology tools and technologies. Due to the large genetic modifications that many yeast strains display, it is highly desirable to avoid the leakage of GMY cells into natural environments and, consequently, the spread of synthetic genes and circuits by horizontal or vertical gene transfer mechanisms within the microorganisms. Moreover, it is also desirable to avoid patented yeast gene technologies spreading outside the production facility. In this review, the different biocontainment technologies currently available for GMYs were evaluated. Interestingly, uniplex-type biocontainment approaches (UTBAs), which rely on nutrient auxotrophies induced by gene mutation or deletion or the expression of the simple kill switches apparatus, are still the major biocontainment approaches in use with GMY. While bacteria such as Escherichia coli account for advanced biocontainment technologies based on synthetic biology and multiplex-type biocontainment approaches (MTBAs), GMYs are distant from this scenario due to many reasons. Thus, a comparison of different UTBAs and MTBAs applied for GMY and genetically engineered microorganisms (GEMs) was made, indicating the major advances of biocontainment techniques for GMYs. |
publishDate |
2023 |
dc.date.issued.fl_str_mv |
2023 |
dc.date.accessioned.fl_str_mv |
2024-03-28T06:23:05Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/274301 |
dc.identifier.issn.pt_BR.fl_str_mv |
2311-5637 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001172016 |
identifier_str_mv |
2311-5637 001172016 |
url |
http://hdl.handle.net/10183/274301 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Fermentation. Switzerland. Vol. 9, no. 4 (Apr. 2023), e341, 16 p. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
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Repositório Institucional da UFRGS |
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Repositório Institucional da UFRGS |
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