Cycle modulation of insulin-like growth factor-binding protein 1 in human endometrium

Detalhes bibliográficos
Autor(a) principal: Corleta, Helena von Eye
Data de Publicação: 2000
Outros Autores: Capp, Edison, Strowitzki, T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/21116
Resumo: Endometrium is one of the fastest growing human tissues. Sex hormones, estrogen and progesterone, in interaction with several growth factors, control its growth and differentiation. Insulin-like growth factor 1 (IGF-1) interacts with cell surface receptors and also with specific soluble binding proteins. IGF-binding proteins (IGF-BP) have been shown to modulate IGF-1 action. Of six known isoforms, IGF-BP-1 has been characterized as a marker produced by endometrial stromal cells in the late secretory phase and in the decidua. In the current study, IGF-1-BP concentration and affinity in the proliferative and secretory phase of the menstrual cycle were measured. Endometrial samples were from patients of reproductive age with regular menstrual cycles and taking no steroid hormones. Cytosolic fractions were prepared and binding of 125I-labeled IGF-1 performed. Crosslinking reaction products were analyzed by SDS-polyacrylamide gel electrophoresis (7.5%) followed by autoradiography. 125I-IGF-1 affinity to cytosolic proteins was not statistically different between the proliferative and secretory endometrium. An approximately 35-kDa binding protein was identified when 125I-IGF-1 was cross-linked to cytosol proteins. Secretory endometrium had significantly more IGF-1- BP when compared to proliferative endometrium. The specificity of the cross-linking process was evaluated by the addition of 100 nM unlabeled IGF-1 or insulin. Unlabeled IGF-1 totally abolished the radioactivity from the band, indicating specific binding. Insulin had no apparent effect on the intensity of the labeled band. These results suggest that IGF-BP could modulate the action of IGF-1 throughout the menstrual cycle. It would be interesting to study this binding protein in other pathologic conditions of the endometrium such as adenocarcinomas and hyperplasia.
id UFRGS-2_986122720df6e66eddc252c027eae001
oai_identifier_str oai:www.lume.ufrgs.br:10183/21116
network_acronym_str UFRGS-2
network_name_str Repositório Institucional da UFRGS
repository_id_str
spelling Corleta, Helena von EyeCapp, EdisonStrowitzki, T.2010-04-24T04:15:23Z20000100-879Xhttp://hdl.handle.net/10183/21116000301852Endometrium is one of the fastest growing human tissues. Sex hormones, estrogen and progesterone, in interaction with several growth factors, control its growth and differentiation. Insulin-like growth factor 1 (IGF-1) interacts with cell surface receptors and also with specific soluble binding proteins. IGF-binding proteins (IGF-BP) have been shown to modulate IGF-1 action. Of six known isoforms, IGF-BP-1 has been characterized as a marker produced by endometrial stromal cells in the late secretory phase and in the decidua. In the current study, IGF-1-BP concentration and affinity in the proliferative and secretory phase of the menstrual cycle were measured. Endometrial samples were from patients of reproductive age with regular menstrual cycles and taking no steroid hormones. Cytosolic fractions were prepared and binding of 125I-labeled IGF-1 performed. Crosslinking reaction products were analyzed by SDS-polyacrylamide gel electrophoresis (7.5%) followed by autoradiography. 125I-IGF-1 affinity to cytosolic proteins was not statistically different between the proliferative and secretory endometrium. An approximately 35-kDa binding protein was identified when 125I-IGF-1 was cross-linked to cytosol proteins. Secretory endometrium had significantly more IGF-1- BP when compared to proliferative endometrium. The specificity of the cross-linking process was evaluated by the addition of 100 nM unlabeled IGF-1 or insulin. Unlabeled IGF-1 totally abolished the radioactivity from the band, indicating specific binding. Insulin had no apparent effect on the intensity of the labeled band. These results suggest that IGF-BP could modulate the action of IGF-1 throughout the menstrual cycle. It would be interesting to study this binding protein in other pathologic conditions of the endometrium such as adenocarcinomas and hyperplasia.application/pdfengBrazilian journal of medical and biological research. Ribeirão Preto, SP. Vol. 33, no. 11 (Nov. 2000), p.1387-1391EndométrioProteína 1 de ligação a fator de crescimento insulin-likeIGF-1Binding proteinEndometriumCycle modulation of insulin-like growth factor-binding protein 1 in human endometriuminfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000301852.pdf000301852.pdfTexto completo (inglês)application/pdf152950http://www.lume.ufrgs.br/bitstream/10183/21116/1/000301852.pdfd46d65199f970e33873b989bcca11682MD51TEXT000301852.pdf.txt000301852.pdf.txtExtracted Texttext/plain17553http://www.lume.ufrgs.br/bitstream/10183/21116/2/000301852.pdf.txtaafdd38c5f1df7154729ca8d24168c28MD52THUMBNAIL000301852.pdf.jpg000301852.pdf.jpgGenerated Thumbnailimage/jpeg1582http://www.lume.ufrgs.br/bitstream/10183/21116/3/000301852.pdf.jpg26f5bd02fd24788f1d5f7d8ceba189d7MD5310183/211162023-06-22 03:31:11.408541oai:www.lume.ufrgs.br:10183/21116Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-06-22T06:31:11Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Cycle modulation of insulin-like growth factor-binding protein 1 in human endometrium
title Cycle modulation of insulin-like growth factor-binding protein 1 in human endometrium
spellingShingle Cycle modulation of insulin-like growth factor-binding protein 1 in human endometrium
Corleta, Helena von Eye
Endométrio
Proteína 1 de ligação a fator de crescimento insulin-like
IGF-1
Binding protein
Endometrium
title_short Cycle modulation of insulin-like growth factor-binding protein 1 in human endometrium
title_full Cycle modulation of insulin-like growth factor-binding protein 1 in human endometrium
title_fullStr Cycle modulation of insulin-like growth factor-binding protein 1 in human endometrium
title_full_unstemmed Cycle modulation of insulin-like growth factor-binding protein 1 in human endometrium
title_sort Cycle modulation of insulin-like growth factor-binding protein 1 in human endometrium
author Corleta, Helena von Eye
author_facet Corleta, Helena von Eye
Capp, Edison
Strowitzki, T.
author_role author
author2 Capp, Edison
Strowitzki, T.
author2_role author
author
dc.contributor.author.fl_str_mv Corleta, Helena von Eye
Capp, Edison
Strowitzki, T.
dc.subject.por.fl_str_mv Endométrio
Proteína 1 de ligação a fator de crescimento insulin-like
topic Endométrio
Proteína 1 de ligação a fator de crescimento insulin-like
IGF-1
Binding protein
Endometrium
dc.subject.eng.fl_str_mv IGF-1
Binding protein
Endometrium
description Endometrium is one of the fastest growing human tissues. Sex hormones, estrogen and progesterone, in interaction with several growth factors, control its growth and differentiation. Insulin-like growth factor 1 (IGF-1) interacts with cell surface receptors and also with specific soluble binding proteins. IGF-binding proteins (IGF-BP) have been shown to modulate IGF-1 action. Of six known isoforms, IGF-BP-1 has been characterized as a marker produced by endometrial stromal cells in the late secretory phase and in the decidua. In the current study, IGF-1-BP concentration and affinity in the proliferative and secretory phase of the menstrual cycle were measured. Endometrial samples were from patients of reproductive age with regular menstrual cycles and taking no steroid hormones. Cytosolic fractions were prepared and binding of 125I-labeled IGF-1 performed. Crosslinking reaction products were analyzed by SDS-polyacrylamide gel electrophoresis (7.5%) followed by autoradiography. 125I-IGF-1 affinity to cytosolic proteins was not statistically different between the proliferative and secretory endometrium. An approximately 35-kDa binding protein was identified when 125I-IGF-1 was cross-linked to cytosol proteins. Secretory endometrium had significantly more IGF-1- BP when compared to proliferative endometrium. The specificity of the cross-linking process was evaluated by the addition of 100 nM unlabeled IGF-1 or insulin. Unlabeled IGF-1 totally abolished the radioactivity from the band, indicating specific binding. Insulin had no apparent effect on the intensity of the labeled band. These results suggest that IGF-BP could modulate the action of IGF-1 throughout the menstrual cycle. It would be interesting to study this binding protein in other pathologic conditions of the endometrium such as adenocarcinomas and hyperplasia.
publishDate 2000
dc.date.issued.fl_str_mv 2000
dc.date.accessioned.fl_str_mv 2010-04-24T04:15:23Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/other
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/21116
dc.identifier.issn.pt_BR.fl_str_mv 0100-879X
dc.identifier.nrb.pt_BR.fl_str_mv 000301852
identifier_str_mv 0100-879X
000301852
url http://hdl.handle.net/10183/21116
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Brazilian journal of medical and biological research. Ribeirão Preto, SP. Vol. 33, no. 11 (Nov. 2000), p.1387-1391
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRGS
instname:Universidade Federal do Rio Grande do Sul (UFRGS)
instacron:UFRGS
instname_str Universidade Federal do Rio Grande do Sul (UFRGS)
instacron_str UFRGS
institution UFRGS
reponame_str Repositório Institucional da UFRGS
collection Repositório Institucional da UFRGS
bitstream.url.fl_str_mv http://www.lume.ufrgs.br/bitstream/10183/21116/1/000301852.pdf
http://www.lume.ufrgs.br/bitstream/10183/21116/2/000301852.pdf.txt
http://www.lume.ufrgs.br/bitstream/10183/21116/3/000301852.pdf.jpg
bitstream.checksum.fl_str_mv d46d65199f970e33873b989bcca11682
aafdd38c5f1df7154729ca8d24168c28
26f5bd02fd24788f1d5f7d8ceba189d7
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)
repository.mail.fl_str_mv
_version_ 1815447406556217344