Redundancy and recombination in the Echinococcus AgB multigene family : is there any similarity with protozoan contigency genes?
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/27460 |
Resumo: | Numerous genetic variants of the Echinococcus antigen B (AgB) are encountered within a single metacestode. This could be a reflection of gene redundancy or the result of a somatic hypermutation process. We evaluate the complexity of the AgB multigene family by characterizing the upstream promoter regions of the 4 already known genes (EgAgB1-EgAgB4) and evaluating their redundancy in the genome of 3 Echinococcus species (E. granulosus, E. ortleppi and E. multilocularis) using PCR-based approaches. We have ascertained that the number of AgB gene copies is quite variable, both within and between species. The most repetitive gene seems to be AgB3, of which there are more than 110 copies in E. ortleppi. For E. granulosus, we have cloned and characterized 10 distinct upstream promoter regions of AgB3 from a single metacestode. Our sequences suggest that AgB1 and AgB3 are involved in gene conversion. These results are discussed in light of the role of gene redundancy and recombination in parasite evasion mechanisms of host immunity, which at present are known for protozoan organisms, but virtually unknown for multicellular parasites. |
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Haag, Karen LuisaGottstein, BrunoMuller, NorbertSchnorr, AndréAyala, Francisco J.2011-01-21T05:59:02Z20060031-1820http://hdl.handle.net/10183/27460000588215Numerous genetic variants of the Echinococcus antigen B (AgB) are encountered within a single metacestode. This could be a reflection of gene redundancy or the result of a somatic hypermutation process. We evaluate the complexity of the AgB multigene family by characterizing the upstream promoter regions of the 4 already known genes (EgAgB1-EgAgB4) and evaluating their redundancy in the genome of 3 Echinococcus species (E. granulosus, E. ortleppi and E. multilocularis) using PCR-based approaches. We have ascertained that the number of AgB gene copies is quite variable, both within and between species. The most repetitive gene seems to be AgB3, of which there are more than 110 copies in E. ortleppi. For E. granulosus, we have cloned and characterized 10 distinct upstream promoter regions of AgB3 from a single metacestode. Our sequences suggest that AgB1 and AgB3 are involved in gene conversion. These results are discussed in light of the role of gene redundancy and recombination in parasite evasion mechanisms of host immunity, which at present are known for protozoan organisms, but virtually unknown for multicellular parasites.application/pdfengParasitology. Cambridge, Inglaterra. Vol. 133, n. 4 (Oct. 2006), p. 411-419EchinococcusHidatidose : Antigenos : ImunodiagnosticoAntígeno BHydatid diseaseAntigen BPromoterRecombinationAntigen diversityReal-time PCRRedundancy and recombination in the Echinococcus AgB multigene family : is there any similarity with protozoan contigency genes?Estrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000588215.pdf000588215.pdfTexto completo (inglês)application/pdf130427http://www.lume.ufrgs.br/bitstream/10183/27460/1/000588215.pdf8bd170933ca19296162cdd3bc371d809MD51TEXT000588215.pdf.txt000588215.pdf.txtExtracted Texttext/plain40235http://www.lume.ufrgs.br/bitstream/10183/27460/2/000588215.pdf.txtc9f7f28bc04d70f4dbc9454767e863b0MD52THUMBNAIL000588215.pdf.jpg000588215.pdf.jpgGenerated Thumbnailimage/jpeg1899http://www.lume.ufrgs.br/bitstream/10183/27460/3/000588215.pdf.jpg59491420ae354fc774fd0f97f0710387MD5310183/274602021-06-13 04:36:31.373613oai:www.lume.ufrgs.br:10183/27460Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-06-13T07:36:31Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Redundancy and recombination in the Echinococcus AgB multigene family : is there any similarity with protozoan contigency genes? |
title |
Redundancy and recombination in the Echinococcus AgB multigene family : is there any similarity with protozoan contigency genes? |
spellingShingle |
Redundancy and recombination in the Echinococcus AgB multigene family : is there any similarity with protozoan contigency genes? Haag, Karen Luisa Echinococcus Hidatidose : Antigenos : Imunodiagnostico Antígeno B Hydatid disease Antigen B Promoter Recombination Antigen diversity Real-time PCR |
title_short |
Redundancy and recombination in the Echinococcus AgB multigene family : is there any similarity with protozoan contigency genes? |
title_full |
Redundancy and recombination in the Echinococcus AgB multigene family : is there any similarity with protozoan contigency genes? |
title_fullStr |
Redundancy and recombination in the Echinococcus AgB multigene family : is there any similarity with protozoan contigency genes? |
title_full_unstemmed |
Redundancy and recombination in the Echinococcus AgB multigene family : is there any similarity with protozoan contigency genes? |
title_sort |
Redundancy and recombination in the Echinococcus AgB multigene family : is there any similarity with protozoan contigency genes? |
author |
Haag, Karen Luisa |
author_facet |
Haag, Karen Luisa Gottstein, Bruno Muller, Norbert Schnorr, André Ayala, Francisco J. |
author_role |
author |
author2 |
Gottstein, Bruno Muller, Norbert Schnorr, André Ayala, Francisco J. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Haag, Karen Luisa Gottstein, Bruno Muller, Norbert Schnorr, André Ayala, Francisco J. |
dc.subject.por.fl_str_mv |
Echinococcus Hidatidose : Antigenos : Imunodiagnostico Antígeno B |
topic |
Echinococcus Hidatidose : Antigenos : Imunodiagnostico Antígeno B Hydatid disease Antigen B Promoter Recombination Antigen diversity Real-time PCR |
dc.subject.eng.fl_str_mv |
Hydatid disease Antigen B Promoter Recombination Antigen diversity Real-time PCR |
description |
Numerous genetic variants of the Echinococcus antigen B (AgB) are encountered within a single metacestode. This could be a reflection of gene redundancy or the result of a somatic hypermutation process. We evaluate the complexity of the AgB multigene family by characterizing the upstream promoter regions of the 4 already known genes (EgAgB1-EgAgB4) and evaluating their redundancy in the genome of 3 Echinococcus species (E. granulosus, E. ortleppi and E. multilocularis) using PCR-based approaches. We have ascertained that the number of AgB gene copies is quite variable, both within and between species. The most repetitive gene seems to be AgB3, of which there are more than 110 copies in E. ortleppi. For E. granulosus, we have cloned and characterized 10 distinct upstream promoter regions of AgB3 from a single metacestode. Our sequences suggest that AgB1 and AgB3 are involved in gene conversion. These results are discussed in light of the role of gene redundancy and recombination in parasite evasion mechanisms of host immunity, which at present are known for protozoan organisms, but virtually unknown for multicellular parasites. |
publishDate |
2006 |
dc.date.issued.fl_str_mv |
2006 |
dc.date.accessioned.fl_str_mv |
2011-01-21T05:59:02Z |
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Estrangeiro info:eu-repo/semantics/article |
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000588215 |
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eng |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Parasitology. Cambridge, Inglaterra. Vol. 133, n. 4 (Oct. 2006), p. 411-419 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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