In vitro effects of Blepharocalyx salicifolius (H.B.K.) O. Berg on the viability of Echinococcus ortleppi protoscoleces

• Autor(a) principal: Noal, Charlise Bolson
• Data de Publicação: 2017
• Outros Autores: Monteiro, Danieli Urach, Brum, Thiele Faccim de, Emmanouilidis, Jéssica, Zanette, Regis Adriel, Morel, Ademir Farias, Stefanon, Eliza Beti de Cassia, Frosi, Marina, La Rue, Mario Luiz de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/225987
• topic: Echinococcus ortleppi; Ácido gálico; Blepharocalyx salicifolius; Protoscolicidal; Scolicidal agent; Gallic acid

Scolicidal agents are important in the treatment of cystic echinococcosis. This study evaluated the scolicidal activity of the plant Blepharocalyx salicifolius (H.B.K.) Berg against Echinococcus ortleppi protoscoleces. The parasite species was identified by amplifying a fragment of the gene cytochrome c oxidase subunit 1 (COX 1). B. salicifolius crude extract at concentrations of 100, 200, 300 and 400 mg/mL was analyzed at different times (5, 10, 15, 30, 45 and 60 min). N-butanol and ethyl acetate fractions (100 and 200 mg/ mL) were also analyzed at 5, 10, 15 and 30 min. Both fractions showed 100% scolicidal activity at the concentration of 200 mg/mL at 5 min. Gallic acid, identified as the major compound of the ethyl acetate fraction- was responsible for the observed scolicidal activity. The results showed that crude extract and fractions of B. salicifolius have scolicidal effect against E. ortleppi protoscoleces.